Syngeneic N1-S1 Orthotopic Hepatocellular Carcinoma in Sprague Dawley Rat for the Development of Interventional Oncology-Based Immunotherapy: Survival Assay and Tumor Immune Microenvironment
Rodent HCC rat models provide advantages for interventional oncology (IO) based immunotherapy research compared to other established larger animal models or mice models. Rapid and predictable tumor growth and affordable costs permit the formation of a compelling preclinical model investigating novel...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/3/913 |
| _version_ | 1797624990447697920 |
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| author | Bongseo Choi Jason Pe Bo Yu Dong-Hyun Kim |
| author_facet | Bongseo Choi Jason Pe Bo Yu Dong-Hyun Kim |
| author_sort | Bongseo Choi |
| collection | DOAJ |
| description | Rodent HCC rat models provide advantages for interventional oncology (IO) based immunotherapy research compared to other established larger animal models or mice models. Rapid and predictable tumor growth and affordable costs permit the formation of a compelling preclinical model investigating novel IO catheter-directed therapies and local ablation therapies. Among orthotopic HCC models, the N1-S1 orthotopic HCC model has been involved in many research cases. Suboptimal tumor induction rates and potential spontaneous regression during tumor implantation procedures discouraged the use of the N1-S1 HCC model in IO-based immunotherapies. Here, N1-S1 HCC models were generated with a subcapsular implantation of two different number of N1-S1 cells using a mini-laporatomy. Tumor growth assay and immunological profiles which can preclinically evaluate the therapeutic efficacy of IO-based immunotherapy, were characterized. Finally, an N1-S1 HCC rat model generated with the proposed procedure demonstrated a representative immune suppressive HCC tumor environment without self-tumor regression. The optimized syngeneic N1-S1 HCC rat models represent an essential tool for pre-clinical evaluation of new IO immunotherapies for the treatment of HCC. |
| first_indexed | 2024-03-11T09:50:30Z |
| format | Article |
| id | doaj.art-d5a52e77cf904ea8ab51580d85ff07c7 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T09:50:30Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-d5a52e77cf904ea8ab51580d85ff07c72023-11-16T16:19:02ZengMDPI AGCancers2072-66942023-01-0115391310.3390/cancers15030913Syngeneic N1-S1 Orthotopic Hepatocellular Carcinoma in Sprague Dawley Rat for the Development of Interventional Oncology-Based Immunotherapy: Survival Assay and Tumor Immune MicroenvironmentBongseo Choi0Jason Pe1Bo Yu2Dong-Hyun Kim3Department of Radiology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USADepartment of Radiology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USADepartment of Radiology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USADepartment of Radiology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USARodent HCC rat models provide advantages for interventional oncology (IO) based immunotherapy research compared to other established larger animal models or mice models. Rapid and predictable tumor growth and affordable costs permit the formation of a compelling preclinical model investigating novel IO catheter-directed therapies and local ablation therapies. Among orthotopic HCC models, the N1-S1 orthotopic HCC model has been involved in many research cases. Suboptimal tumor induction rates and potential spontaneous regression during tumor implantation procedures discouraged the use of the N1-S1 HCC model in IO-based immunotherapies. Here, N1-S1 HCC models were generated with a subcapsular implantation of two different number of N1-S1 cells using a mini-laporatomy. Tumor growth assay and immunological profiles which can preclinically evaluate the therapeutic efficacy of IO-based immunotherapy, were characterized. Finally, an N1-S1 HCC rat model generated with the proposed procedure demonstrated a representative immune suppressive HCC tumor environment without self-tumor regression. The optimized syngeneic N1-S1 HCC rat models represent an essential tool for pre-clinical evaluation of new IO immunotherapies for the treatment of HCC.https://www.mdpi.com/2072-6694/15/3/913hepatocellular carcinoma (HCC)rodent HCC animal modelcancer immunotherapyinterventional oncology |
| spellingShingle | Bongseo Choi Jason Pe Bo Yu Dong-Hyun Kim Syngeneic N1-S1 Orthotopic Hepatocellular Carcinoma in Sprague Dawley Rat for the Development of Interventional Oncology-Based Immunotherapy: Survival Assay and Tumor Immune Microenvironment Cancers hepatocellular carcinoma (HCC) rodent HCC animal model cancer immunotherapy interventional oncology |
| title | Syngeneic N1-S1 Orthotopic Hepatocellular Carcinoma in Sprague Dawley Rat for the Development of Interventional Oncology-Based Immunotherapy: Survival Assay and Tumor Immune Microenvironment |
| title_full | Syngeneic N1-S1 Orthotopic Hepatocellular Carcinoma in Sprague Dawley Rat for the Development of Interventional Oncology-Based Immunotherapy: Survival Assay and Tumor Immune Microenvironment |
| title_fullStr | Syngeneic N1-S1 Orthotopic Hepatocellular Carcinoma in Sprague Dawley Rat for the Development of Interventional Oncology-Based Immunotherapy: Survival Assay and Tumor Immune Microenvironment |
| title_full_unstemmed | Syngeneic N1-S1 Orthotopic Hepatocellular Carcinoma in Sprague Dawley Rat for the Development of Interventional Oncology-Based Immunotherapy: Survival Assay and Tumor Immune Microenvironment |
| title_short | Syngeneic N1-S1 Orthotopic Hepatocellular Carcinoma in Sprague Dawley Rat for the Development of Interventional Oncology-Based Immunotherapy: Survival Assay and Tumor Immune Microenvironment |
| title_sort | syngeneic n1 s1 orthotopic hepatocellular carcinoma in sprague dawley rat for the development of interventional oncology based immunotherapy survival assay and tumor immune microenvironment |
| topic | hepatocellular carcinoma (HCC) rodent HCC animal model cancer immunotherapy interventional oncology |
| url | https://www.mdpi.com/2072-6694/15/3/913 |
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