The Effect of Endosulfan Pesticide in Some Biochemical Parameters of white Mice
The present study aimed to examine the effect of endosulfan insecticide on some molecular and biochemical parameters in white mice. Thirty mice were separated randomly into three groups for treatment with endosulfan. One group (G1) served as the control, while the other two groups received intraper...
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| Format: | Article |
| Language: | English |
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University of Baghdad
2023-01-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| Subjects: | |
| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/2981 |
| _version_ | 1828047714025209856 |
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| author | Diyana M. Abd-Alkazem Adel M. Rabee |
| author_facet | Diyana M. Abd-Alkazem Adel M. Rabee |
| author_sort | Diyana M. Abd-Alkazem |
| collection | DOAJ |
| description |
The present study aimed to examine the effect of endosulfan insecticide on some molecular and biochemical parameters in white mice. Thirty mice were separated randomly into three groups for treatment with endosulfan. One group (G1) served as the control, while the other two groups received intraperitoneal injections of endosulfan G2 (3 mg/kg) and G3 (17 mg/kg) twice a week for 21 and 45 days, respectively. A biochemical study by measuring liver function parameters, including (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and kidney function parameters, including (Blood Urea and Creatinine) and malondialdehyde (MDA), catalase activity (CAT). This study also tested DNA damage by comet assay (normal%, low%, medium%, high%). The results of renal function parameters (Blood Urea and Creatinine) were significantly increased in all treated groups after 21 and 45 days exposed to endosulfan compared with control groups. The highest value of blood urea recorded was (49.33 ±0.88 mg/dl) at 17 mg/kg for 45 days compared with the control group, and the highest value of Creatinine recorded was (1.81 ±0.13 mg/dl) at 17 mg/kg for 45 days compared with the control group. Liver function parameters (ALT and AST) significantly increased in all treated groups compared with control groups. The results of MDA, CAT enzyme, were significantly increased in all treated groups after 21 and 45 days compared with control groups. The highest value of MDA recorded was (3.93 ±0.07 μM) at 17 mg/kg for 45 days compared with the control group. Tail DNA (%) showed a significant increase at high concentrations, and the results showed a considerable increase in the severe damage of DNA in the treated group 17 mg\kg b.wt. (25.00 ±1.00)% for 45 days, compared with the control group (3.00 ±1.00) %.
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| first_indexed | 2024-04-10T18:46:32Z |
| format | Article |
| id | doaj.art-d5a964f882bb4b828a2cbe60395fbdb7 |
| institution | Directory Open Access Journal |
| issn | 1609-4042 2521-3407 |
| language | English |
| last_indexed | 2024-04-10T18:46:32Z |
| publishDate | 2023-01-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj.art-d5a964f882bb4b828a2cbe60395fbdb72023-02-01T10:25:41ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072023-01-0136110.30526/36.1.29814352The Effect of Endosulfan Pesticide in Some Biochemical Parameters of white MiceDiyana M. Abd-Alkazem0Adel M. Rabee1Department of Biology, College of Sciences for Women, University of Baghdad, Baghdad, IraqDepartment of Biology, College of Sciences for Women, University of Baghdad, Baghdad, Iraq The present study aimed to examine the effect of endosulfan insecticide on some molecular and biochemical parameters in white mice. Thirty mice were separated randomly into three groups for treatment with endosulfan. One group (G1) served as the control, while the other two groups received intraperitoneal injections of endosulfan G2 (3 mg/kg) and G3 (17 mg/kg) twice a week for 21 and 45 days, respectively. A biochemical study by measuring liver function parameters, including (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and kidney function parameters, including (Blood Urea and Creatinine) and malondialdehyde (MDA), catalase activity (CAT). This study also tested DNA damage by comet assay (normal%, low%, medium%, high%). The results of renal function parameters (Blood Urea and Creatinine) were significantly increased in all treated groups after 21 and 45 days exposed to endosulfan compared with control groups. The highest value of blood urea recorded was (49.33 ±0.88 mg/dl) at 17 mg/kg for 45 days compared with the control group, and the highest value of Creatinine recorded was (1.81 ±0.13 mg/dl) at 17 mg/kg for 45 days compared with the control group. Liver function parameters (ALT and AST) significantly increased in all treated groups compared with control groups. The results of MDA, CAT enzyme, were significantly increased in all treated groups after 21 and 45 days compared with control groups. The highest value of MDA recorded was (3.93 ±0.07 μM) at 17 mg/kg for 45 days compared with the control group. Tail DNA (%) showed a significant increase at high concentrations, and the results showed a considerable increase in the severe damage of DNA in the treated group 17 mg\kg b.wt. (25.00 ±1.00)% for 45 days, compared with the control group (3.00 ±1.00) %. https://jih.uobaghdad.edu.iq/index.php/j/article/view/2981Liver functionComet assayCreatinine,sub-lethal doses |
| spellingShingle | Diyana M. Abd-Alkazem Adel M. Rabee The Effect of Endosulfan Pesticide in Some Biochemical Parameters of white Mice Ibn Al-Haitham Journal for Pure and Applied Sciences Liver function Comet assay Creatinine, sub-lethal doses |
| title | The Effect of Endosulfan Pesticide in Some Biochemical Parameters of white Mice |
| title_full | The Effect of Endosulfan Pesticide in Some Biochemical Parameters of white Mice |
| title_fullStr | The Effect of Endosulfan Pesticide in Some Biochemical Parameters of white Mice |
| title_full_unstemmed | The Effect of Endosulfan Pesticide in Some Biochemical Parameters of white Mice |
| title_short | The Effect of Endosulfan Pesticide in Some Biochemical Parameters of white Mice |
| title_sort | effect of endosulfan pesticide in some biochemical parameters of white mice |
| topic | Liver function Comet assay Creatinine, sub-lethal doses |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/2981 |
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