3D AFM Nanomechanical Characterization of Biological Materials
Atomic Force Microscopy (AFM) is a powerful tool enabling the mechanical characterization of biological materials at the nanoscale. Since biological materials are highly heterogeneous, their mechanical characterization is still considered to be a challenging procedure. In this paper, a new approach...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/3/395 |
| _version_ | 1797623719843069952 |
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| author | Stylianos Vasileios Kontomaris Andreas Stylianou Anastasios Georgakopoulos Anna Malamou |
| author_facet | Stylianos Vasileios Kontomaris Andreas Stylianou Anastasios Georgakopoulos Anna Malamou |
| author_sort | Stylianos Vasileios Kontomaris |
| collection | DOAJ |
| description | Atomic Force Microscopy (AFM) is a powerful tool enabling the mechanical characterization of biological materials at the nanoscale. Since biological materials are highly heterogeneous, their mechanical characterization is still considered to be a challenging procedure. In this paper, a new approach that leads to a 3-dimensional (3D) nanomechanical characterization is presented based on the average Young’s modulus and the AFM indentation method. The proposed method can contribute to the clarification of the variability of the mechanical properties of biological samples in the 3-dimensional space (variability at the x–y plane and depth-dependent behavior). The method was applied to agarose gels, fibroblasts, and breast cancer cells. Moreover, new mathematical methods towards a quantitative mechanical characterization are also proposed. The presented approach is a step forward to a more accurate and complete characterization of biological materials and could contribute to an accurate user-independent diagnosis of various diseases such as cancer in the future. |
| first_indexed | 2024-03-11T09:32:48Z |
| format | Article |
| id | doaj.art-d5aace5e96f04fb68379253794a6a35f |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T09:32:48Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-d5aace5e96f04fb68379253794a6a35f2023-11-16T17:34:06ZengMDPI AGNanomaterials2079-49912023-01-0113339510.3390/nano130303953D AFM Nanomechanical Characterization of Biological MaterialsStylianos Vasileios Kontomaris0Andreas Stylianou1Anastasios Georgakopoulos2Anna Malamou3BioNanoTec Ltd., 2043 Nicosia, CyprusSchool of Sciences, European University Cyprus, 2404 Nicosia, CyprusSchool of Electrical and Computer Engineering, National Technical University of Athens, 15780 Athens, GreeceSchool of Electrical and Computer Engineering, National Technical University of Athens, 15780 Athens, GreeceAtomic Force Microscopy (AFM) is a powerful tool enabling the mechanical characterization of biological materials at the nanoscale. Since biological materials are highly heterogeneous, their mechanical characterization is still considered to be a challenging procedure. In this paper, a new approach that leads to a 3-dimensional (3D) nanomechanical characterization is presented based on the average Young’s modulus and the AFM indentation method. The proposed method can contribute to the clarification of the variability of the mechanical properties of biological samples in the 3-dimensional space (variability at the x–y plane and depth-dependent behavior). The method was applied to agarose gels, fibroblasts, and breast cancer cells. Moreover, new mathematical methods towards a quantitative mechanical characterization are also proposed. The presented approach is a step forward to a more accurate and complete characterization of biological materials and could contribute to an accurate user-independent diagnosis of various diseases such as cancer in the future.https://www.mdpi.com/2079-4991/13/3/395average young’s modulusnanoindentationscanning probe microscopymechanical propertiesdepth-dependent behaviorcells |
| spellingShingle | Stylianos Vasileios Kontomaris Andreas Stylianou Anastasios Georgakopoulos Anna Malamou 3D AFM Nanomechanical Characterization of Biological Materials Nanomaterials average young’s modulus nanoindentation scanning probe microscopy mechanical properties depth-dependent behavior cells |
| title | 3D AFM Nanomechanical Characterization of Biological Materials |
| title_full | 3D AFM Nanomechanical Characterization of Biological Materials |
| title_fullStr | 3D AFM Nanomechanical Characterization of Biological Materials |
| title_full_unstemmed | 3D AFM Nanomechanical Characterization of Biological Materials |
| title_short | 3D AFM Nanomechanical Characterization of Biological Materials |
| title_sort | 3d afm nanomechanical characterization of biological materials |
| topic | average young’s modulus nanoindentation scanning probe microscopy mechanical properties depth-dependent behavior cells |
| url | https://www.mdpi.com/2079-4991/13/3/395 |
| work_keys_str_mv | AT stylianosvasileioskontomaris 3dafmnanomechanicalcharacterizationofbiologicalmaterials AT andreasstylianou 3dafmnanomechanicalcharacterizationofbiologicalmaterials AT anastasiosgeorgakopoulos 3dafmnanomechanicalcharacterizationofbiologicalmaterials AT annamalamou 3dafmnanomechanicalcharacterizationofbiologicalmaterials |