Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus–Oocyte Complex Activity through TLR4-Derived ERK1/2 Signaling in Mice
Peroxiredoxins (PRDXs) are expressed in the ovary and during ovulation. PRDX1 activity related to the immuno-like response during ovulation is unknown. We investigated the roles of <i>Prdx1</i> on TLR4 and ERK1/2 signaling from the ovulated cumulus–oocyte complex (COC) using <i>Prd...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/17/9437 |
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| author | Hyo-Jin Park Bokyung Kim Deog-Bon Koo Dong-Seok Lee |
| author_facet | Hyo-Jin Park Bokyung Kim Deog-Bon Koo Dong-Seok Lee |
| author_sort | Hyo-Jin Park |
| collection | DOAJ |
| description | Peroxiredoxins (PRDXs) are expressed in the ovary and during ovulation. PRDX1 activity related to the immuno-like response during ovulation is unknown. We investigated the roles of <i>Prdx1</i> on TLR4 and ERK1/2 signaling from the ovulated cumulus–oocyte complex (COC) using <i>Prdx1</i>-knockout (K/O) and wild-type (WT) mice. Ovulated COCs were collected 12 and 16 h after pregnant mare serum gonadotropin/hCG injection. PRDX1 protein expression and COC secretion factors (<i>Il-6</i>, <i>Tnfaip6</i>, and <i>Ptgs2</i>) increased 16 h after ovulated COCs of the WT mice were obtained. We treated the ovulated COCs in mice with LPS (0.5 μg/mL) or hyaluronidase (Hya) (10 units/mL) to induce TLR4 activity. Intracellular reactive oxygen species (ROS), cumulus cell apoptosis, PRDX1, TLR4/P38/ERK1/2 protein expression, and COC secretion factors’ mRNA levels increased in LPS- and Hya-treated COCs. The ERK inhibitor (U0126) and <i>Prdx1</i> siRNA affected TLR4/ERK1/2 expression. The number and cumulus expansion of ovulated COCs by ROS were impaired in <i>Prdx1</i> K/O mice but not in WT ones. <i>Prdx1</i> gene deletion induced TLR4/P38/ERK1/2 expression and cumulus expansion genes. These results show the controlling roles of PRDX1 for TLR4/P38/ERK1/2 signaling activity in ovulated mice and the interlink of COCs with ovulation. |
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| last_indexed | 2024-03-10T08:11:11Z |
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| spelling | doaj.art-d5ad90f82b884538b32a7e0d3fec0d6c2023-11-22T10:43:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012217943710.3390/ijms22179437Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus–Oocyte Complex Activity through TLR4-Derived ERK1/2 Signaling in MiceHyo-Jin Park0Bokyung Kim1Deog-Bon Koo2Dong-Seok Lee3Department of Biotechnology, College of Engineering, Daegu University, 201 Daegudae-ro, Jillyang, Gyeongsan, Gyeongbuk 38453, KoreaBK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, KoreaDepartment of Biotechnology, College of Engineering, Daegu University, 201 Daegudae-ro, Jillyang, Gyeongsan, Gyeongbuk 38453, KoreaBK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, KoreaPeroxiredoxins (PRDXs) are expressed in the ovary and during ovulation. PRDX1 activity related to the immuno-like response during ovulation is unknown. We investigated the roles of <i>Prdx1</i> on TLR4 and ERK1/2 signaling from the ovulated cumulus–oocyte complex (COC) using <i>Prdx1</i>-knockout (K/O) and wild-type (WT) mice. Ovulated COCs were collected 12 and 16 h after pregnant mare serum gonadotropin/hCG injection. PRDX1 protein expression and COC secretion factors (<i>Il-6</i>, <i>Tnfaip6</i>, and <i>Ptgs2</i>) increased 16 h after ovulated COCs of the WT mice were obtained. We treated the ovulated COCs in mice with LPS (0.5 μg/mL) or hyaluronidase (Hya) (10 units/mL) to induce TLR4 activity. Intracellular reactive oxygen species (ROS), cumulus cell apoptosis, PRDX1, TLR4/P38/ERK1/2 protein expression, and COC secretion factors’ mRNA levels increased in LPS- and Hya-treated COCs. The ERK inhibitor (U0126) and <i>Prdx1</i> siRNA affected TLR4/ERK1/2 expression. The number and cumulus expansion of ovulated COCs by ROS were impaired in <i>Prdx1</i> K/O mice but not in WT ones. <i>Prdx1</i> gene deletion induced TLR4/P38/ERK1/2 expression and cumulus expansion genes. These results show the controlling roles of PRDX1 for TLR4/P38/ERK1/2 signaling activity in ovulated mice and the interlink of COCs with ovulation.https://www.mdpi.com/1422-0067/22/17/9437cumulus–oocyte complexes (COCs)extracellular signal-regulated kinase (ERK)miceovulationperoxiredoxin 1 (PRDX1)toll-like receptor4 (TLR4) |
| spellingShingle | Hyo-Jin Park Bokyung Kim Deog-Bon Koo Dong-Seok Lee Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus–Oocyte Complex Activity through TLR4-Derived ERK1/2 Signaling in Mice International Journal of Molecular Sciences cumulus–oocyte complexes (COCs) extracellular signal-regulated kinase (ERK) mice ovulation peroxiredoxin 1 (PRDX1) toll-like receptor4 (TLR4) |
| title | Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus–Oocyte Complex Activity through TLR4-Derived ERK1/2 Signaling in Mice |
| title_full | Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus–Oocyte Complex Activity through TLR4-Derived ERK1/2 Signaling in Mice |
| title_fullStr | Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus–Oocyte Complex Activity through TLR4-Derived ERK1/2 Signaling in Mice |
| title_full_unstemmed | Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus–Oocyte Complex Activity through TLR4-Derived ERK1/2 Signaling in Mice |
| title_short | Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus–Oocyte Complex Activity through TLR4-Derived ERK1/2 Signaling in Mice |
| title_sort | peroxiredoxin 1 controls ovulation and ovulated cumulus oocyte complex activity through tlr4 derived erk1 2 signaling in mice |
| topic | cumulus–oocyte complexes (COCs) extracellular signal-regulated kinase (ERK) mice ovulation peroxiredoxin 1 (PRDX1) toll-like receptor4 (TLR4) |
| url | https://www.mdpi.com/1422-0067/22/17/9437 |
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