Association between faecal load of <it>lawsonia intracellularis</it> and pathological findings of proliferative enteropathy in pigs with diarrhoea
<p>Abstract</p> <p>Background</p> <p>The study was designed to investigate correlation between histological findings of <it>Lawsonia intracellularis</it> in porcine cases of diarrhoea and the quantitative detection of <it>Lawsonia intracellularis</i...
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BMC
2012-10-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://www.biomedcentral.com/1746-6148/8/198 |
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| author | Pedersen Ken Ståhl Marie Guedes Roberto Maurício Angen Øystein Nielsen Jens Jensen Tim K |
| author_facet | Pedersen Ken Ståhl Marie Guedes Roberto Maurício Angen Øystein Nielsen Jens Jensen Tim K |
| author_sort | Pedersen Ken |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The study was designed to investigate correlation between histological findings of <it>Lawsonia intracellularis</it> in porcine cases of diarrhoea and the quantitative detection of <it>Lawsonia intracellularis</it> in faeces. A total of 156 pigs (10 to 70 days post weaning) with diarrhoea were randomly selected from 20 herds: The pigs were subjected to necropsy, histopathology, immunohistochemistry and faecal quantification of <it>Lawsonia intracellularis</it> by real time PCR.</p> <p>Results</p> <p>The median <it>Lawsonia intracellularis</it> excretion was significantly higher in pigs with gross lesions of proliferative enteropathy (median excretion: 5.92 log<sub>10</sub> bacteria/g faeces) compared to pigs without gross lesions of proliferative enteropathy (median excretion: <3.3 log<sub>10</sub> bacteria/g faeces) (<it>P</it><0.001). Spearman’s correlation coefficient between the measureable PE lesions and <it>L. intracellularis</it> excretion was 0.50 (<it>P</it><0.001). A significantly increasing trend in <it>Lawsonia intracellularis</it> excretion level for increasing proliferative enteropathy histopathology and immunohistochemistry scores was demonstrated (<it>P</it><0.001; <it>P</it><0.001). Spearman’s correlation coefficient between the histopathology scores and <it>L. intracellularis</it> excretion was 0.67 (<it>P</it><0.001). Spearman’s correlation coefficient between the IHC scores and <it>L. intracellularis</it> excretion was 0.77 (<it>P</it><0.001).</p> <p>Conclusions</p> <p>The histological and quantitative PCR detection of <it>Lawsonia intracellularis</it> were correlated in pigs with diarrhoea. Overall the results suggest that clinically important levels for <it>Lawsonia intracellularis</it> excretion in faeces may be established. Such clinical threshold levels may be used in practice to confirm a diagnosis of <it>Lawsonia intracellularis</it> associated diarrhoea.</p> |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
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| spelling | doaj.art-d5ae51898c374f318a9d362596c5a60a2022-12-22T03:05:19ZengBMCBMC Veterinary Research1746-61482012-10-018119810.1186/1746-6148-8-198Association between faecal load of <it>lawsonia intracellularis</it> and pathological findings of proliferative enteropathy in pigs with diarrhoeaPedersen KenStåhl MarieGuedes Roberto MaurícioAngen ØysteinNielsen JensJensen Tim K<p>Abstract</p> <p>Background</p> <p>The study was designed to investigate correlation between histological findings of <it>Lawsonia intracellularis</it> in porcine cases of diarrhoea and the quantitative detection of <it>Lawsonia intracellularis</it> in faeces. A total of 156 pigs (10 to 70 days post weaning) with diarrhoea were randomly selected from 20 herds: The pigs were subjected to necropsy, histopathology, immunohistochemistry and faecal quantification of <it>Lawsonia intracellularis</it> by real time PCR.</p> <p>Results</p> <p>The median <it>Lawsonia intracellularis</it> excretion was significantly higher in pigs with gross lesions of proliferative enteropathy (median excretion: 5.92 log<sub>10</sub> bacteria/g faeces) compared to pigs without gross lesions of proliferative enteropathy (median excretion: <3.3 log<sub>10</sub> bacteria/g faeces) (<it>P</it><0.001). Spearman’s correlation coefficient between the measureable PE lesions and <it>L. intracellularis</it> excretion was 0.50 (<it>P</it><0.001). A significantly increasing trend in <it>Lawsonia intracellularis</it> excretion level for increasing proliferative enteropathy histopathology and immunohistochemistry scores was demonstrated (<it>P</it><0.001; <it>P</it><0.001). Spearman’s correlation coefficient between the histopathology scores and <it>L. intracellularis</it> excretion was 0.67 (<it>P</it><0.001). Spearman’s correlation coefficient between the IHC scores and <it>L. intracellularis</it> excretion was 0.77 (<it>P</it><0.001).</p> <p>Conclusions</p> <p>The histological and quantitative PCR detection of <it>Lawsonia intracellularis</it> were correlated in pigs with diarrhoea. Overall the results suggest that clinically important levels for <it>Lawsonia intracellularis</it> excretion in faeces may be established. Such clinical threshold levels may be used in practice to confirm a diagnosis of <it>Lawsonia intracellularis</it> associated diarrhoea.</p>http://www.biomedcentral.com/1746-6148/8/198DiarrhoeaImmunohistochemistry<it>Lawsonia intracellularis</it>Quantitative real time PCRPig |
| spellingShingle | Pedersen Ken Ståhl Marie Guedes Roberto Maurício Angen Øystein Nielsen Jens Jensen Tim K Association between faecal load of <it>lawsonia intracellularis</it> and pathological findings of proliferative enteropathy in pigs with diarrhoea BMC Veterinary Research Diarrhoea Immunohistochemistry <it>Lawsonia intracellularis</it> Quantitative real time PCR Pig |
| title | Association between faecal load of <it>lawsonia intracellularis</it> and pathological findings of proliferative enteropathy in pigs with diarrhoea |
| title_full | Association between faecal load of <it>lawsonia intracellularis</it> and pathological findings of proliferative enteropathy in pigs with diarrhoea |
| title_fullStr | Association between faecal load of <it>lawsonia intracellularis</it> and pathological findings of proliferative enteropathy in pigs with diarrhoea |
| title_full_unstemmed | Association between faecal load of <it>lawsonia intracellularis</it> and pathological findings of proliferative enteropathy in pigs with diarrhoea |
| title_short | Association between faecal load of <it>lawsonia intracellularis</it> and pathological findings of proliferative enteropathy in pigs with diarrhoea |
| title_sort | association between faecal load of it lawsonia intracellularis it and pathological findings of proliferative enteropathy in pigs with diarrhoea |
| topic | Diarrhoea Immunohistochemistry <it>Lawsonia intracellularis</it> Quantitative real time PCR Pig |
| url | http://www.biomedcentral.com/1746-6148/8/198 |
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