Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride
The action of stem cells is mediated by their paracrine secretions which comprise the secretory profile. Various approaches can be used to modify the secretory profile of stem cells. Creating a hypoxic environment is one method. The present study aims to demonstrate the influence of CoCl<sub>2...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Journal of Personalized Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4426/11/4/247 |
| _version_ | 1797539579722465280 |
|---|---|
| author | Shilpa Bhandi Ahmed Al Kahtani Mohammed Mashyakhy Loai Alsofi Prabhadevi C. Maganur Satish Vishwanathaiah Luca Testarelli Andrea Del Giudice Deepak Mehta Nishant Vyas Vikrant R. Patil A. Thirumal Raj Shankargouda Patil |
| author_facet | Shilpa Bhandi Ahmed Al Kahtani Mohammed Mashyakhy Loai Alsofi Prabhadevi C. Maganur Satish Vishwanathaiah Luca Testarelli Andrea Del Giudice Deepak Mehta Nishant Vyas Vikrant R. Patil A. Thirumal Raj Shankargouda Patil |
| author_sort | Shilpa Bhandi |
| collection | DOAJ |
| description | The action of stem cells is mediated by their paracrine secretions which comprise the secretory profile. Various approaches can be used to modify the secretory profile of stem cells. Creating a hypoxic environment is one method. The present study aims to demonstrate the influence of CoCl<sub>2</sub> in generating hypoxic conditions in a dental pulp stem cell (DPSCs) culture, and the effect of this environment on their secretory profile. DPSCs that were isolated from human permanent teeth were characterized and treated with different concentrations of CoCl<sub>2</sub> to assess their viability by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and proliferation by a cell counting kit (CCK)-8 assay. The gene expression level of hypoxia-inducible factor 1-alpha (HIF-1α) was analyzed by quantitative real time polymerase chain reaction (qRT-PCR) to demonstrate a hypoxic environment. Comparative evaluation of the growth factors and cytokines were done by cytometric bead array. Gene expression levels of transcription factors OCT4 and SOX2 were analyzed by qRT-PCR to understand the effect of CoCl<sub>2</sub> on stemness in DPSCs. DPSCs were positive for MSC-specific markers. Doses of CoCl<sub>2,</sub> up to 20 µM, did not negatively affect cell viability; in low doses (5 µM), it promoted cell survival. Treatment with 10 µM of CoCl<sub>2</sub> significantly augmented the genetic expression of HIF-1α. Cells treated with 10 µM of CoCl<sub>2</sub> showed changes in the levels of growth factors and cytokines produced. It was very evident that CoCl<sub>2</sub> also increased the expression of OCT4 and SOX2, which is the modulation of stemness of DPSCs. A CoCl<sub>2</sub> treatment-induced hypoxic environment modulates the secretory profile of DPSCs. |
| first_indexed | 2024-03-10T12:47:50Z |
| format | Article |
| id | doaj.art-d5b346a554c3485eb749f352d6a1c90b |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-10T12:47:50Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-d5b346a554c3485eb749f352d6a1c90b2023-11-21T13:20:51ZengMDPI AGJournal of Personalized Medicine2075-44262021-03-0111424710.3390/jpm11040247Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt ChlorideShilpa Bhandi0Ahmed Al Kahtani1Mohammed Mashyakhy2Loai Alsofi3Prabhadevi C. Maganur4Satish Vishwanathaiah5Luca Testarelli6Andrea Del Giudice7Deepak Mehta8Nishant Vyas9Vikrant R. Patil10A. Thirumal Raj11Shankargouda Patil12Department of Restorative Dental Sciences, College of Dentistry, Jazan University, Jazan 45142, Saudi ArabiaDepartment of Restorative Dental Sciences, College of Dentistry, King Saud Universirty, Riyadh 11451, Saudi ArabiaDepartment of Restorative Dental Sciences, College of Dentistry, Jazan University, Jazan 45142, Saudi ArabiaDepartment of Endodontics, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Preventive Dental Sciences, Division of Pedodontics, College of Dentistry, Jazan University, Jazan 45142, Saudi ArabiaDepartment of Preventive Dental Sciences, Division of Pedodontics, College of Dentistry, Jazan University, Jazan 45142, Saudi ArabiaDepartment of Oral and Maxillofacial Sciences, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Oral and Maxillofacial Sciences, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Preventive and Restorative Dentistry, College of Dental Medicine, University of Sharjah, Sharjah 27272, United Arab EmiratesLogical Life Science Private Limited, Pune 411041, IndiaLogical Life Science Private Limited, Pune 411041, IndiaDepartment of Oral Pathology and Microbiology, Sri Venkateswara Dental College and Hospital, Chennai 600130, IndiaDepartment of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan 45142, Saudi ArabiaThe action of stem cells is mediated by their paracrine secretions which comprise the secretory profile. Various approaches can be used to modify the secretory profile of stem cells. Creating a hypoxic environment is one method. The present study aims to demonstrate the influence of CoCl<sub>2</sub> in generating hypoxic conditions in a dental pulp stem cell (DPSCs) culture, and the effect of this environment on their secretory profile. DPSCs that were isolated from human permanent teeth were characterized and treated with different concentrations of CoCl<sub>2</sub> to assess their viability by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and proliferation by a cell counting kit (CCK)-8 assay. The gene expression level of hypoxia-inducible factor 1-alpha (HIF-1α) was analyzed by quantitative real time polymerase chain reaction (qRT-PCR) to demonstrate a hypoxic environment. Comparative evaluation of the growth factors and cytokines were done by cytometric bead array. Gene expression levels of transcription factors OCT4 and SOX2 were analyzed by qRT-PCR to understand the effect of CoCl<sub>2</sub> on stemness in DPSCs. DPSCs were positive for MSC-specific markers. Doses of CoCl<sub>2,</sub> up to 20 µM, did not negatively affect cell viability; in low doses (5 µM), it promoted cell survival. Treatment with 10 µM of CoCl<sub>2</sub> significantly augmented the genetic expression of HIF-1α. Cells treated with 10 µM of CoCl<sub>2</sub> showed changes in the levels of growth factors and cytokines produced. It was very evident that CoCl<sub>2</sub> also increased the expression of OCT4 and SOX2, which is the modulation of stemness of DPSCs. A CoCl<sub>2</sub> treatment-induced hypoxic environment modulates the secretory profile of DPSCs.https://www.mdpi.com/2075-4426/11/4/247cobalt chloridedental pulp stem cellshypoxia inducible factorsecretory profilestemness |
| spellingShingle | Shilpa Bhandi Ahmed Al Kahtani Mohammed Mashyakhy Loai Alsofi Prabhadevi C. Maganur Satish Vishwanathaiah Luca Testarelli Andrea Del Giudice Deepak Mehta Nishant Vyas Vikrant R. Patil A. Thirumal Raj Shankargouda Patil Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride Journal of Personalized Medicine cobalt chloride dental pulp stem cells hypoxia inducible factor secretory profile stemness |
| title | Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride |
| title_full | Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride |
| title_fullStr | Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride |
| title_full_unstemmed | Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride |
| title_short | Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride |
| title_sort | modulation of the dental pulp stem cell secretory profile by hypoxia induction using cobalt chloride |
| topic | cobalt chloride dental pulp stem cells hypoxia inducible factor secretory profile stemness |
| url | https://www.mdpi.com/2075-4426/11/4/247 |
| work_keys_str_mv | AT shilpabhandi modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT ahmedalkahtani modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT mohammedmashyakhy modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT loaialsofi modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT prabhadevicmaganur modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT satishvishwanathaiah modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT lucatestarelli modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT andreadelgiudice modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT deepakmehta modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT nishantvyas modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT vikrantrpatil modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT athirumalraj modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride AT shankargoudapatil modulationofthedentalpulpstemcellsecretoryprofilebyhypoxiainductionusingcobaltchloride |