Detection and molecular characterization of tomato big bud disease in Qazvin province
Some samples were collected from tomato fields in Qazvin from tomato plants with big bud symptoms such as plant droop and purplish vein under the leaf, enlarged and sac-like pistils and malformed buds. DNA was extracted from the veins and vascular tissues of the plant with CTAB-based methods. In sym...
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2019-08-01
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| Series: | Journal of Crop Protection |
| Subjects: | |
| Online Access: | http://jcp.modares.ac.ir/article-3-30554-en.html |
| _version_ | 1818551409463787520 |
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| author | Abbas Davoodi Naser Panjekeh Kobra Moslemkhani Abdolhossein Taheri |
| author_facet | Abbas Davoodi Naser Panjekeh Kobra Moslemkhani Abdolhossein Taheri |
| author_sort | Abbas Davoodi |
| collection | DOAJ |
| description | Some samples were collected from tomato fields in Qazvin from tomato plants with big bud symptoms such as plant droop and purplish vein under the leaf, enlarged and sac-like pistils and malformed buds. DNA was extracted from the veins and vascular tissues of the plant with CTAB-based methods. In symptomatic plants, DNA fragments of 1800 and 1200bp were amplified by PCR using P1/P7, R16F2n/R16R2 primers. Restriction fragment length polymorphism (RFLP) analysis of nested R16F2n/R16R2 primed PCR product (1200bp) showed that the tomato big bud phytoplasma from Qazvin (TOM-Qazvin) is a member of clover proliferation (16SrVI). Phylogenetic analysis of 16SrRNA and putative restriction site analysis of the R16F2n/R16R2 primed sequence classified TOM phytoplasma in clover proliferation (16SrVI) group and belonged to subgroup 16SrVI-A. Virtual RFLP by using 1200bp sequencing of 16SRNA and 17 restriction enzymes confirmed that TOM-Qazvin belonged to the subgroup 16SrVI-A and16SrVI group. To our knowledge, this is the first report of tomato big bud disease in Qazvin province. |
| first_indexed | 2024-12-12T08:59:31Z |
| format | Article |
| id | doaj.art-d5b4a9b449b140ba8a8761a3ebb9d899 |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-12-12T08:59:31Z |
| publishDate | 2019-08-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-d5b4a9b449b140ba8a8761a3ebb9d8992022-12-22T00:29:52ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2019-08-0184379388Detection and molecular characterization of tomato big bud disease in Qazvin provinceAbbas Davoodi0Naser Panjekeh1Kobra Moslemkhani2Abdolhossein Taheri3 Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran. Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran. Seed and Plant Certification and Registration Institute, AREEO, Karaj, Iran. Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Some samples were collected from tomato fields in Qazvin from tomato plants with big bud symptoms such as plant droop and purplish vein under the leaf, enlarged and sac-like pistils and malformed buds. DNA was extracted from the veins and vascular tissues of the plant with CTAB-based methods. In symptomatic plants, DNA fragments of 1800 and 1200bp were amplified by PCR using P1/P7, R16F2n/R16R2 primers. Restriction fragment length polymorphism (RFLP) analysis of nested R16F2n/R16R2 primed PCR product (1200bp) showed that the tomato big bud phytoplasma from Qazvin (TOM-Qazvin) is a member of clover proliferation (16SrVI). Phylogenetic analysis of 16SrRNA and putative restriction site analysis of the R16F2n/R16R2 primed sequence classified TOM phytoplasma in clover proliferation (16SrVI) group and belonged to subgroup 16SrVI-A. Virtual RFLP by using 1200bp sequencing of 16SRNA and 17 restriction enzymes confirmed that TOM-Qazvin belonged to the subgroup 16SrVI-A and16SrVI group. To our knowledge, this is the first report of tomato big bud disease in Qazvin province.http://jcp.modares.ac.ir/article-3-30554-en.htmlbig budiphyclassifierrflptomatophytoplasma |
| spellingShingle | Abbas Davoodi Naser Panjekeh Kobra Moslemkhani Abdolhossein Taheri Detection and molecular characterization of tomato big bud disease in Qazvin province Journal of Crop Protection big bud iphyclassifier rflp tomato phytoplasma |
| title | Detection and molecular characterization of tomato big bud disease in Qazvin province |
| title_full | Detection and molecular characterization of tomato big bud disease in Qazvin province |
| title_fullStr | Detection and molecular characterization of tomato big bud disease in Qazvin province |
| title_full_unstemmed | Detection and molecular characterization of tomato big bud disease in Qazvin province |
| title_short | Detection and molecular characterization of tomato big bud disease in Qazvin province |
| title_sort | detection and molecular characterization of tomato big bud disease in qazvin province |
| topic | big bud iphyclassifier rflp tomato phytoplasma |
| url | http://jcp.modares.ac.ir/article-3-30554-en.html |
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