Studies of cryopreservation of callus of Citrus suavissima Hort. et Tanaka by vitrification(瓯柑愈伤组织的玻璃化法超低温保存研究)
采用玻璃化法对瓯柑愈伤组织进行超低温保存研究.瓯柑愈伤组织在含5%二甲亚砜(DMSO)的MS培养基上预培养5 d，室温下60%PVS2预处理20 min，然后用100%PVS2于0 ℃处理40 min，投入液氮(LN)保存，24 h后在40 ℃水浴中迅速化冻，再用1.2 mol/L蔗糖培养液洗涤3次.用氯化三苯四氮唑(TTC)法检测，其存活率可达85. 62%.冻后的愈伤组织转移到MS + 6-BA 0.5 mg/L继代培养基上，在黑暗中培养生长，存活率可达76. 32%.分析讨论了瓯柑愈伤组织玻璃化法超低温保存中存在的一些问题及意义，并对其前景进行了展望....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2004-03-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2004.31.2.197-201 |
| _version_ | 1797236409222823936 |
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| author | CHENYong(陈勇) CHENXian-ting(陈娴婷) WANGJun-hui(王君晖) |
| author_facet | CHENYong(陈勇) CHENXian-ting(陈娴婷) WANGJun-hui(王君晖) |
| author_sort | CHENYong(陈勇) |
| collection | DOAJ |
| description | 采用玻璃化法对瓯柑愈伤组织进行超低温保存研究.瓯柑愈伤组织在含5%二甲亚砜(DMSO)的MS培养基上预培养5 d，室温下60%PVS2预处理20 min，然后用100%PVS2于0 ℃处理40 min，投入液氮(LN)保存，24 h后在40 ℃水浴中迅速化冻，再用1.2 mol/L蔗糖培养液洗涤3次.用氯化三苯四氮唑(TTC)法检测，其存活率可达85. 62%.冻后的愈伤组织转移到MS + 6-BA 0.5 mg/L继代培养基上，在黑暗中培养生长，存活率可达76. 32%.分析讨论了瓯柑愈伤组织玻璃化法超低温保存中存在的一些问题及意义，并对其前景进行了展望. |
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| format | Article |
| id | doaj.art-d5b4c0fb8f6440f589969f065b2e6ad0 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:03:23Z |
| publishDate | 2004-03-01 |
| publisher | Zhejiang University Press |
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| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-d5b4c0fb8f6440f589969f065b2e6ad02024-03-29T01:58:21ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972004-03-01312197201zjup/1008-9497.2004.31.2.197-201Studies of cryopreservation of callus of Citrus suavissima Hort. et Tanaka by vitrification(瓯柑愈伤组织的玻璃化法超低温保存研究)CHENYong(陈勇)0CHENXian-ting(陈娴婷)1WANGJun-hui(王君晖)2 1.Department of Biology and Environment Science, Wenzhou Normal College, Wenzhou 325027, China( 1.温州师范学院生物与环境科学系，浙江 温州 325027) 1.Department of Biology and Environment Science, Wenzhou Normal College, Wenzhou 325027, China( 1.温州师范学院生物与环境科学系，浙江 温州 325027) 2.College of life Sciences, Zhejiang University, Hangzhou 310028, China( 2.浙江大学生命科学学院，浙江 杭州 310028)采用玻璃化法对瓯柑愈伤组织进行超低温保存研究.瓯柑愈伤组织在含5%二甲亚砜(DMSO)的MS培养基上预培养5 d，室温下60%PVS2预处理20 min，然后用100%PVS2于0 ℃处理40 min，投入液氮(LN)保存，24 h后在40 ℃水浴中迅速化冻，再用1.2 mol/L蔗糖培养液洗涤3次.用氯化三苯四氮唑(TTC)法检测，其存活率可达85. 62%.冻后的愈伤组织转移到MS + 6-BA 0.5 mg/L继代培养基上，在黑暗中培养生长，存活率可达76. 32%.分析讨论了瓯柑愈伤组织玻璃化法超低温保存中存在的一些问题及意义，并对其前景进行了展望.https://doi.org/zjup/1008-9497.2004.31.2.197-201瓯柑愈伤组织玻璃化法超低温保存 |
| spellingShingle | CHENYong(陈勇) CHENXian-ting(陈娴婷) WANGJun-hui(王君晖) Studies of cryopreservation of callus of Citrus suavissima Hort. et Tanaka by vitrification(瓯柑愈伤组织的玻璃化法超低温保存研究) Zhejiang Daxue xuebao. Lixue ban 瓯柑 愈伤组织 玻璃化法 超低温保存 |
| title | Studies of cryopreservation of callus of Citrus suavissima Hort. et Tanaka by vitrification(瓯柑愈伤组织的玻璃化法超低温保存研究) |
| title_full | Studies of cryopreservation of callus of Citrus suavissima Hort. et Tanaka by vitrification(瓯柑愈伤组织的玻璃化法超低温保存研究) |
| title_fullStr | Studies of cryopreservation of callus of Citrus suavissima Hort. et Tanaka by vitrification(瓯柑愈伤组织的玻璃化法超低温保存研究) |
| title_full_unstemmed | Studies of cryopreservation of callus of Citrus suavissima Hort. et Tanaka by vitrification(瓯柑愈伤组织的玻璃化法超低温保存研究) |
| title_short | Studies of cryopreservation of callus of Citrus suavissima Hort. et Tanaka by vitrification(瓯柑愈伤组织的玻璃化法超低温保存研究) |
| title_sort | studies of cryopreservation of callus of citrus suavissima hort et tanaka by vitrification 瓯柑愈伤组织的玻璃化法超低温保存研究 |
| topic | 瓯柑 愈伤组织 玻璃化法 超低温保存 |
| url | https://doi.org/zjup/1008-9497.2004.31.2.197-201 |
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