Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine
The extracellular guanosine 5′-triphosphate, GTP, has been demonstrated to be an enhancer of myogenic cell differentiation in a murine cell line, not yet in human muscle cells. Our hypothesis was that GTP could influence also human skeletal muscle regeneration, specifically in the first phases. We t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2018.00152/full |
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| author | Tiziana Pietrangelo Ester S. Di Filippo Marcello Locatelli Francesco Piacenza Marco Farina Eleonora Pavoni Andrea Di Donato Denise Innosa Mauro Provinciali Stefania Fulle |
| author_facet | Tiziana Pietrangelo Ester S. Di Filippo Marcello Locatelli Francesco Piacenza Marco Farina Eleonora Pavoni Andrea Di Donato Denise Innosa Mauro Provinciali Stefania Fulle |
| author_sort | Tiziana Pietrangelo |
| collection | DOAJ |
| description | The extracellular guanosine 5′-triphosphate, GTP, has been demonstrated to be an enhancer of myogenic cell differentiation in a murine cell line, not yet in human muscle cells. Our hypothesis was that GTP could influence also human skeletal muscle regeneration, specifically in the first phases. We tested GTP stimulus on human muscle precursor cells established in culture by human satellite cells derived from Vastus Lateralis of three young male. Our data show that extracellular GTP (a) up-regulated miRNA (specifically miR133a and miR133b) and myogenic regulator factor and (b) induces human myogenic precursor cells to release exosomes stuffed with guanosine based molecules (mainly guanosine) in the extracellular milieu. We think that probably these exosomes could be addressed to influence by means of their content (mainly guanosine) in paracrine or autocrine manner the surrounding cells and/or at distance other muscles or tissues. |
| first_indexed | 2024-12-13T08:00:47Z |
| format | Article |
| id | doaj.art-d5b59689ffa84b82928483fe0f15e947 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-13T08:00:47Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-d5b59689ffa84b82928483fe0f15e9472022-12-21T23:54:26ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-03-01910.3389/fphar.2018.00152330526Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With GuanosineTiziana Pietrangelo0Ester S. Di Filippo1Marcello Locatelli2Francesco Piacenza3Marco Farina4Eleonora Pavoni5Andrea Di Donato6Denise Innosa7Mauro Provinciali8Stefania Fulle9Dipartimento Neuroscienze Imaging and Scienze Cliniche, Università degli Studi “G. d’Annunzio” Chieti-Pescara, Chieti, ItalyDipartimento Neuroscienze Imaging and Scienze Cliniche, Università degli Studi “G. d’Annunzio” Chieti-Pescara, Chieti, ItalyDipartimento di Farmacia, Università degli Studi “G. d’Annunzio” Chieti-Pescara, Chieti, ItalyIRCCS-Istituto Nazionale di Riposo e Cura per Anziani, Polo Scientifico e Tecnologico, Centro di Tecnologie Avanzate nell’Invecchiamento, Ancona, ItalyDipartimento di Ingegneria dell’Informazione, Università Politecnica delle Marche, Ancona, ItalyDipartimento di Ingegneria dell’Informazione, Università Politecnica delle Marche, Ancona, ItalyHarvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, United StatesFacoltà di Bioscienze e Tecnologie Agro-Alimentari e Ambientali, Università di Teramo, Teramo, ItalyIRCCS-Istituto Nazionale di Riposo e Cura per Anziani, Polo Scientifico e Tecnologico, Centro di Tecnologie Avanzate nell’Invecchiamento, Ancona, ItalyDipartimento Neuroscienze Imaging and Scienze Cliniche, Università degli Studi “G. d’Annunzio” Chieti-Pescara, Chieti, ItalyThe extracellular guanosine 5′-triphosphate, GTP, has been demonstrated to be an enhancer of myogenic cell differentiation in a murine cell line, not yet in human muscle cells. Our hypothesis was that GTP could influence also human skeletal muscle regeneration, specifically in the first phases. We tested GTP stimulus on human muscle precursor cells established in culture by human satellite cells derived from Vastus Lateralis of three young male. Our data show that extracellular GTP (a) up-regulated miRNA (specifically miR133a and miR133b) and myogenic regulator factor and (b) induces human myogenic precursor cells to release exosomes stuffed with guanosine based molecules (mainly guanosine) in the extracellular milieu. We think that probably these exosomes could be addressed to influence by means of their content (mainly guanosine) in paracrine or autocrine manner the surrounding cells and/or at distance other muscles or tissues.http://journal.frontiersin.org/article/10.3389/fphar.2018.00152/fullsatellite cellsguanosineguanosine 5′-triphosphatemyomiRNAexosomesskeletal muscle regeneration |
| spellingShingle | Tiziana Pietrangelo Ester S. Di Filippo Marcello Locatelli Francesco Piacenza Marco Farina Eleonora Pavoni Andrea Di Donato Denise Innosa Mauro Provinciali Stefania Fulle Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine Frontiers in Pharmacology satellite cells guanosine guanosine 5′-triphosphate myomiRNA exosomes skeletal muscle regeneration |
| title | Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine |
| title_full | Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine |
| title_fullStr | Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine |
| title_full_unstemmed | Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine |
| title_short | Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine |
| title_sort | extracellular guanosine 5 triphosphate induces human muscle satellite cells to release exosomes stuffed with guanosine |
| topic | satellite cells guanosine guanosine 5′-triphosphate myomiRNA exosomes skeletal muscle regeneration |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2018.00152/full |
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