Protective effects of melatonin on testis histology following acute torsion-detorsion in rats
Background: Testicular damage due to spermatic cord torsion may lead toinfertility. It is probably because of changes in oxidative stress factors such asmalondialdehyde. Objective: To investigate the protective effect of melatonin (MLT), as anantioxidant, on testicular damage induced by acute unilat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Sciences
2017-05-01
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| Series: | International Journal of Reproductive BioMedicine |
| Subjects: | |
| Online Access: | http://journals.ssu.ac.ir/ijrmnew/article-1-809-en.html |
| _version_ | 1819211595522244608 |
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| author | Mehri Mirhoseini Fereshteh Talebpour Amiri Abbas Ali Karimpour Malekshah Zahra Rezanejad Gatabi Elmira Ghaffari |
| author_facet | Mehri Mirhoseini Fereshteh Talebpour Amiri Abbas Ali Karimpour Malekshah Zahra Rezanejad Gatabi Elmira Ghaffari |
| author_sort | Mehri Mirhoseini |
| collection | DOAJ |
| description | Background: Testicular damage due to spermatic cord torsion may lead toinfertility. It is probably because of changes in oxidative stress factors such asmalondialdehyde.
Objective: To investigate the protective effect of melatonin (MLT), as anantioxidant, on testicular damage induced by acute unilateral spermatic cord torsionand detorsion (T/D) in rats.
Materials and Methods: In this experimental study, 48 adult male Wistar rats wererandomly divided into three groups (8 rats/group): sham group underwent rightscrotal surgery only., the T/D group underwent right testicular torsion (for 1 hr) anddetorsion, and the melatonin group underwent right testicular torsion, received 25μg/kg melatonin intraperitoneally immediately after surgery of T/D. Then thehistological parameters and malondialdehyde (MDA) changes were evaluated.
Results: Torsion and detorsion decreased the diameter of the tubules significantlycompared to controls (p=0.003). Melatonin could increase the diameter, but it wasnot significant (p=0.26). The heights of the epithelium were constant in sham, T/D,and melatonin groups without any significant difference between groups (p=0.98).Based on Johnsen’s score, spermatogenesis was normal in the sham group. Thetorsion significantly injured all lineage cells (p<0.001). There was no any spermatidor sperm in the seminiferous tubules. Melatonin improved the spermatogenesissignificantly (p=0.02), but could not improve MDA level significantly (p=0.99).
Conclusion: Severe degenerative changes of testis were induced by acute unilateralspermatic cord torsion and detorsion in rats, but it had no effect on MDA level. |
| first_indexed | 2024-12-23T06:29:34Z |
| format | Article |
| id | doaj.art-d5b84b8a176e4904a4837297bdd440e4 |
| institution | Directory Open Access Journal |
| issn | 2476-4108 2476-3772 |
| language | English |
| last_indexed | 2024-12-23T06:29:34Z |
| publishDate | 2017-05-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-d5b84b8a176e4904a4837297bdd440e42022-12-21T17:56:58ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722017-05-01153141146Protective effects of melatonin on testis histology following acute torsion-detorsion in ratsMehri Mirhoseini0Fereshteh Talebpour Amiri1Abbas Ali Karimpour Malekshah2Zahra Rezanejad Gatabi3Elmira Ghaffari4 Amol Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran. Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran epartment of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Background: Testicular damage due to spermatic cord torsion may lead toinfertility. It is probably because of changes in oxidative stress factors such asmalondialdehyde. Objective: To investigate the protective effect of melatonin (MLT), as anantioxidant, on testicular damage induced by acute unilateral spermatic cord torsionand detorsion (T/D) in rats. Materials and Methods: In this experimental study, 48 adult male Wistar rats wererandomly divided into three groups (8 rats/group): sham group underwent rightscrotal surgery only., the T/D group underwent right testicular torsion (for 1 hr) anddetorsion, and the melatonin group underwent right testicular torsion, received 25μg/kg melatonin intraperitoneally immediately after surgery of T/D. Then thehistological parameters and malondialdehyde (MDA) changes were evaluated. Results: Torsion and detorsion decreased the diameter of the tubules significantlycompared to controls (p=0.003). Melatonin could increase the diameter, but it wasnot significant (p=0.26). The heights of the epithelium were constant in sham, T/D,and melatonin groups without any significant difference between groups (p=0.98).Based on Johnsen’s score, spermatogenesis was normal in the sham group. Thetorsion significantly injured all lineage cells (p<0.001). There was no any spermatidor sperm in the seminiferous tubules. Melatonin improved the spermatogenesissignificantly (p=0.02), but could not improve MDA level significantly (p=0.99). Conclusion: Severe degenerative changes of testis were induced by acute unilateralspermatic cord torsion and detorsion in rats, but it had no effect on MDA level.http://journals.ssu.ac.ir/ijrmnew/article-1-809-en.htmlspermatic cord torsiontestismelatoninmdarats. |
| spellingShingle | Mehri Mirhoseini Fereshteh Talebpour Amiri Abbas Ali Karimpour Malekshah Zahra Rezanejad Gatabi Elmira Ghaffari Protective effects of melatonin on testis histology following acute torsion-detorsion in rats International Journal of Reproductive BioMedicine spermatic cord torsion testis melatonin mda rats. |
| title | Protective effects of melatonin on testis histology following acute torsion-detorsion in rats |
| title_full | Protective effects of melatonin on testis histology following acute torsion-detorsion in rats |
| title_fullStr | Protective effects of melatonin on testis histology following acute torsion-detorsion in rats |
| title_full_unstemmed | Protective effects of melatonin on testis histology following acute torsion-detorsion in rats |
| title_short | Protective effects of melatonin on testis histology following acute torsion-detorsion in rats |
| title_sort | protective effects of melatonin on testis histology following acute torsion detorsion in rats |
| topic | spermatic cord torsion testis melatonin mda rats. |
| url | http://journals.ssu.ac.ir/ijrmnew/article-1-809-en.html |
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