Orientin Reduces Myocardial Infarction Size via eNOS/NO Signaling and Thus Mitigates Adverse Cardiac Remodeling
Orientin is a flavonoid extracted from Chinese traditional herb, Polygonum orientale L. Previous study has reported that orientin protected myocardial from ischemia reperfusion injury. However, whether orientin could protect against cardiac remodeling after myocardial injury remains unclear. The aim...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-12-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2017.00926/full |
| _version_ | 1818311365165580288 |
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| author | Fangfang Li Fangfang Li Jing Zong Jing Zong Hao Zhang Hao Zhang Peijie Zhang Luhong Xu Luhong Xu Kai Liang Kai Liang Lu Yang Lu Yang Hui Yong Hui Yong Wenhao Qian Wenhao Qian |
| author_facet | Fangfang Li Fangfang Li Jing Zong Jing Zong Hao Zhang Hao Zhang Peijie Zhang Luhong Xu Luhong Xu Kai Liang Kai Liang Lu Yang Lu Yang Hui Yong Hui Yong Wenhao Qian Wenhao Qian |
| author_sort | Fangfang Li |
| collection | DOAJ |
| description | Orientin is a flavonoid extracted from Chinese traditional herb, Polygonum orientale L. Previous study has reported that orientin protected myocardial from ischemia reperfusion injury. However, whether orientin could protect against cardiac remodeling after myocardial injury remains unclear. The aim of our study is to investigate the effects of orientin in the progression of cardiac remodeling after myocardial infarction (MI). Mice cardiac remodeling model was established by left coronary artery ligation surgery. Experimental groups were as follows: vehicle-sham, orientin-sham, vehicle-MI, and orientin-MI. Animals were treated with vehicle or orientin (40 mg/kg) for 25 days starting 3 days after surgery. After 4 weeks of MI, mice with orientin treatment had decreased mortality and improved cardiac function. Significantly, at 4 weeks post-MI, orientin treatment decreased fibrosis, inflammatory response, and cardiomyocyte apoptosis. Furthermore, orientin treatment attenuated the hypoxia-induced neonatal rat cardiomyocyte apoptosis and increased cell viability. Additionally, orientin supplementation mitigated oxidative stress in remodeling heart tissue and cardiomyocytes exposed to hypoxia as measured by 2′,7′-dichlorodihydrofluorescein diacetate fluorescent probe. Mechanistically, orientin promotes cardioprotection by activating the eNOS/NO signaling cascades, which was confirmed by eNOS inhibitor (L-NAME) in vitro and in vivo. Inhibition of oxidative stress by orientin via eNOS/NO signaling cascades in the heart may represent a potential therapy for cardiac remodeling. |
| first_indexed | 2024-12-13T08:00:48Z |
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| id | doaj.art-d5ba33069fa14bb192748b9aa8e1aa15 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-13T08:00:48Z |
| publishDate | 2017-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-d5ba33069fa14bb192748b9aa8e1aa152022-12-21T23:54:26ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122017-12-01810.3389/fphar.2017.00926314138Orientin Reduces Myocardial Infarction Size via eNOS/NO Signaling and Thus Mitigates Adverse Cardiac RemodelingFangfang Li0Fangfang Li1Jing Zong2Jing Zong3Hao Zhang4Hao Zhang5Peijie Zhang6Luhong Xu7Luhong Xu8Kai Liang9Kai Liang10Lu Yang11Lu Yang12Hui Yong13Hui Yong14Wenhao Qian15Wenhao Qian16Department of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou, ChinaDepartment of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou, ChinaDepartment of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou, ChinaEmergency Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaDepartment of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou, ChinaDepartment of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou, ChinaDepartment of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou, ChinaDepartment of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou, ChinaDepartment of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Cardiovascular Disease Research, Xuzhou Medical University, Xuzhou, ChinaOrientin is a flavonoid extracted from Chinese traditional herb, Polygonum orientale L. Previous study has reported that orientin protected myocardial from ischemia reperfusion injury. However, whether orientin could protect against cardiac remodeling after myocardial injury remains unclear. The aim of our study is to investigate the effects of orientin in the progression of cardiac remodeling after myocardial infarction (MI). Mice cardiac remodeling model was established by left coronary artery ligation surgery. Experimental groups were as follows: vehicle-sham, orientin-sham, vehicle-MI, and orientin-MI. Animals were treated with vehicle or orientin (40 mg/kg) for 25 days starting 3 days after surgery. After 4 weeks of MI, mice with orientin treatment had decreased mortality and improved cardiac function. Significantly, at 4 weeks post-MI, orientin treatment decreased fibrosis, inflammatory response, and cardiomyocyte apoptosis. Furthermore, orientin treatment attenuated the hypoxia-induced neonatal rat cardiomyocyte apoptosis and increased cell viability. Additionally, orientin supplementation mitigated oxidative stress in remodeling heart tissue and cardiomyocytes exposed to hypoxia as measured by 2′,7′-dichlorodihydrofluorescein diacetate fluorescent probe. Mechanistically, orientin promotes cardioprotection by activating the eNOS/NO signaling cascades, which was confirmed by eNOS inhibitor (L-NAME) in vitro and in vivo. Inhibition of oxidative stress by orientin via eNOS/NO signaling cascades in the heart may represent a potential therapy for cardiac remodeling.http://journal.frontiersin.org/article/10.3389/fphar.2017.00926/fullorientinmyocardial infarctioncardiac remodelingeNOSnitric oxidereactive oxygen species |
| spellingShingle | Fangfang Li Fangfang Li Jing Zong Jing Zong Hao Zhang Hao Zhang Peijie Zhang Luhong Xu Luhong Xu Kai Liang Kai Liang Lu Yang Lu Yang Hui Yong Hui Yong Wenhao Qian Wenhao Qian Orientin Reduces Myocardial Infarction Size via eNOS/NO Signaling and Thus Mitigates Adverse Cardiac Remodeling Frontiers in Pharmacology orientin myocardial infarction cardiac remodeling eNOS nitric oxide reactive oxygen species |
| title | Orientin Reduces Myocardial Infarction Size via eNOS/NO Signaling and Thus Mitigates Adverse Cardiac Remodeling |
| title_full | Orientin Reduces Myocardial Infarction Size via eNOS/NO Signaling and Thus Mitigates Adverse Cardiac Remodeling |
| title_fullStr | Orientin Reduces Myocardial Infarction Size via eNOS/NO Signaling and Thus Mitigates Adverse Cardiac Remodeling |
| title_full_unstemmed | Orientin Reduces Myocardial Infarction Size via eNOS/NO Signaling and Thus Mitigates Adverse Cardiac Remodeling |
| title_short | Orientin Reduces Myocardial Infarction Size via eNOS/NO Signaling and Thus Mitigates Adverse Cardiac Remodeling |
| title_sort | orientin reduces myocardial infarction size via enos no signaling and thus mitigates adverse cardiac remodeling |
| topic | orientin myocardial infarction cardiac remodeling eNOS nitric oxide reactive oxygen species |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2017.00926/full |
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