Short‐term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in Thoroughbreds
Abstract The aim of this study was to investigate effects of short‐term hypoxic training on lactate metabolism in the gluteus medius muscle of Thoroughbreds. Using crossover design (3 months washout), eight Thoroughbred horses were trained for 2 weeks in normoxia (FIO2 = 21%) and hypoxia (FIO2 = 18%...
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| Format: | Article |
| Language: | English |
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Wiley
2020-06-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.14473 |
| _version_ | 1818500574905106432 |
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| author | Wenxin Wang Kazutaka Mukai Kenya Takahashi Hajime Ohmura Toshiyuki Takahashi Hideo Hatta Yu Kitaoka |
| author_facet | Wenxin Wang Kazutaka Mukai Kenya Takahashi Hajime Ohmura Toshiyuki Takahashi Hideo Hatta Yu Kitaoka |
| author_sort | Wenxin Wang |
| collection | DOAJ |
| description | Abstract The aim of this study was to investigate effects of short‐term hypoxic training on lactate metabolism in the gluteus medius muscle of Thoroughbreds. Using crossover design (3 months washout), eight Thoroughbred horses were trained for 2 weeks in normoxia (FIO2 = 21%) and hypoxia (FIO2 = 18%) each. They ran at 95% maximal oxygen consumption (V̇O2max) on a treadmill inclined at 6% for 2 min (3 days/week) measured under normoxia. Before and after each training period, all horses were subjected to an incremental exercise test (IET) under normoxia. Following the 2‐week trainings, V̇O2max in IET increased significantly under both oxygen conditions. The exercise duration in IET increased significantly only after hypoxic training. The monocarboxylate transporter (MCT) 1 protein levels remained unchanged after training under both oxygen conditions, whereas MCT4 protein levels increased significantly after training in hypoxia but not after training in normoxia. Phosphofructokinase activity increased significantly only after hypoxic training, whereas cytochrome c oxidase activity increased significantly only after normoxic training. Our results suggest that hypoxic training efficiently enhances glycolytic capacity and levels of the lactate transporter protein MCT4, which facilitates lactate efflux from the skeletal muscle. |
| first_indexed | 2024-12-10T20:44:36Z |
| format | Article |
| id | doaj.art-d5bb620603084b9583fc1c2c9c8d9816 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-10T20:44:36Z |
| publishDate | 2020-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-d5bb620603084b9583fc1c2c9c8d98162022-12-22T01:34:17ZengWileyPhysiological Reports2051-817X2020-06-01811n/an/a10.14814/phy2.14473Short‐term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in ThoroughbredsWenxin Wang0Kazutaka Mukai1Kenya Takahashi2Hajime Ohmura3Toshiyuki Takahashi4Hideo Hatta5Yu Kitaoka6Department of Human Sciences Kanagawa University Kanagawa JapanEquine Research InstituteJapan Racing Association Tochigi JapanDepartment of Sports Sciences The University of Tokyo Tokyo JapanEquine Research InstituteJapan Racing Association Tochigi JapanEquine Research InstituteJapan Racing Association Tochigi JapanDepartment of Sports Sciences The University of Tokyo Tokyo JapanDepartment of Human Sciences Kanagawa University Kanagawa JapanAbstract The aim of this study was to investigate effects of short‐term hypoxic training on lactate metabolism in the gluteus medius muscle of Thoroughbreds. Using crossover design (3 months washout), eight Thoroughbred horses were trained for 2 weeks in normoxia (FIO2 = 21%) and hypoxia (FIO2 = 18%) each. They ran at 95% maximal oxygen consumption (V̇O2max) on a treadmill inclined at 6% for 2 min (3 days/week) measured under normoxia. Before and after each training period, all horses were subjected to an incremental exercise test (IET) under normoxia. Following the 2‐week trainings, V̇O2max in IET increased significantly under both oxygen conditions. The exercise duration in IET increased significantly only after hypoxic training. The monocarboxylate transporter (MCT) 1 protein levels remained unchanged after training under both oxygen conditions, whereas MCT4 protein levels increased significantly after training in hypoxia but not after training in normoxia. Phosphofructokinase activity increased significantly only after hypoxic training, whereas cytochrome c oxidase activity increased significantly only after normoxic training. Our results suggest that hypoxic training efficiently enhances glycolytic capacity and levels of the lactate transporter protein MCT4, which facilitates lactate efflux from the skeletal muscle.https://doi.org/10.14814/phy2.14473hypoxialactatemonocarboxylate transporterskeletal muscle |
| spellingShingle | Wenxin Wang Kazutaka Mukai Kenya Takahashi Hajime Ohmura Toshiyuki Takahashi Hideo Hatta Yu Kitaoka Short‐term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in Thoroughbreds Physiological Reports hypoxia lactate monocarboxylate transporter skeletal muscle |
| title | Short‐term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in Thoroughbreds |
| title_full | Short‐term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in Thoroughbreds |
| title_fullStr | Short‐term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in Thoroughbreds |
| title_full_unstemmed | Short‐term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in Thoroughbreds |
| title_short | Short‐term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in Thoroughbreds |
| title_sort | short term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in thoroughbreds |
| topic | hypoxia lactate monocarboxylate transporter skeletal muscle |
| url | https://doi.org/10.14814/phy2.14473 |
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