Molecular screening and characterization of shiga toxin-producing escherichia coli by multiplex pcr assays for stx1, stx2, eaea, h7 in raw milk
The presence of Shiga-toxin producing Escherichia coli (STEC) was evaluated in bulk milk from dairy cattle farms in Bulgaria. From 20 regional farms only 4 were selected as a source for milk sampling on the basis of their annual STEC shedding in feces. All the samples were PCR analyzed after selecti...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2018-12-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2459 |
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| author | Koycho KOEV Georgi ZHELEV Plamen MARUTSOV Krasimira GOSPODINOVA Vladimir PETROV Todor STOYANCHEV |
| author_facet | Koycho KOEV Georgi ZHELEV Plamen MARUTSOV Krasimira GOSPODINOVA Vladimir PETROV Todor STOYANCHEV |
| author_sort | Koycho KOEV |
| collection | DOAJ |
| description | The presence of Shiga-toxin producing Escherichia coli (STEC) was evaluated in bulk milk from dairy cattle farms in Bulgaria. From 20 regional
farms only 4 were selected as a source for milk sampling on the basis of their annual STEC shedding in feces. All the samples were PCR
analyzed after selective enrichment. Positive samples were detected in subsequent molecular testing as: two of samples (9.08%) were
positive for stx1, one sample (4.54%) for stx2, 10 samples (45.54%) for eae and 12 (54.54%) for H7 coding genes. |
| first_indexed | 2024-03-13T04:38:44Z |
| format | Article |
| id | doaj.art-d5bbaf18e94d4357bc5f578461961066 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:44Z |
| publishDate | 2018-12-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-d5bbaf18e94d4357bc5f5784619610662023-06-19T06:34:42ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512018-12-0125227127510.9775/kvfd.2018.203732459Molecular screening and characterization of shiga toxin-producing escherichia coli by multiplex pcr assays for stx1, stx2, eaea, h7 in raw milkKoycho KOEV0Georgi ZHELEV1Plamen MARUTSOV2Krasimira GOSPODINOVA3Vladimir PETROV4Todor STOYANCHEV5Trakia University, Department of veterinary microbiology, infectious and parsitic diseases, Stara Zagora, BULGARIATrakia University, Department of veterinary microbiology, infectious and parsitic diseases, Stara Zagora, BULGARIATrakia University, Department of veterinary microbiology, infectious and parsitic diseases, Stara Zagora, BULGARIATrakia University, Department of veterinary microbiology, infectious and parsitic diseases, Stara Zagora, BULGARIATrakia University, Department of veterinary microbiology, infectious and parsitic diseases, Stara Zagora, BULGARIATrakia University, Department of Food Hygiene, Technology and Control, Veterinary Legislation and Management, Stara Zagora, BULGARIAThe presence of Shiga-toxin producing Escherichia coli (STEC) was evaluated in bulk milk from dairy cattle farms in Bulgaria. From 20 regional farms only 4 were selected as a source for milk sampling on the basis of their annual STEC shedding in feces. All the samples were PCR analyzed after selective enrichment. Positive samples were detected in subsequent molecular testing as: two of samples (9.08%) were positive for stx1, one sample (4.54%) for stx2, 10 samples (45.54%) for eae and 12 (54.54%) for H7 coding genes.https://vetdergikafkas.org/pdf.php?id=2459escherichia coli o157: н7dairy farmsbulk milkenrichmentpcr |
| spellingShingle | Koycho KOEV Georgi ZHELEV Plamen MARUTSOV Krasimira GOSPODINOVA Vladimir PETROV Todor STOYANCHEV Molecular screening and characterization of shiga toxin-producing escherichia coli by multiplex pcr assays for stx1, stx2, eaea, h7 in raw milk Kafkas Universitesi Veteriner Fakültesi Dergisi escherichia coli o157: н7 dairy farms bulk milk enrichment pcr |
| title | Molecular screening and characterization of shiga toxin-producing escherichia coli by multiplex pcr assays for stx1, stx2, eaea, h7 in raw milk |
| title_full | Molecular screening and characterization of shiga toxin-producing escherichia coli by multiplex pcr assays for stx1, stx2, eaea, h7 in raw milk |
| title_fullStr | Molecular screening and characterization of shiga toxin-producing escherichia coli by multiplex pcr assays for stx1, stx2, eaea, h7 in raw milk |
| title_full_unstemmed | Molecular screening and characterization of shiga toxin-producing escherichia coli by multiplex pcr assays for stx1, stx2, eaea, h7 in raw milk |
| title_short | Molecular screening and characterization of shiga toxin-producing escherichia coli by multiplex pcr assays for stx1, stx2, eaea, h7 in raw milk |
| title_sort | molecular screening and characterization of shiga toxin producing escherichia coli by multiplex pcr assays for stx1 stx2 eaea h7 in raw milk |
| topic | escherichia coli o157: н7 dairy farms bulk milk enrichment pcr |
| url | https://vetdergikafkas.org/pdf.php?id=2459 |
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