Influence of Pre-Analytic Conditions on Quantity of Lymphocytes
Lymphocytes are key players in the pathogenesis of multiple sclerosis and a distinct target of several immunomodulatory treatment strategies. In this study, we aim to evaluate the effect of various pre-analytic conditions on immune cell counts to conclude the relevance for clinical implications. Twe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/17/13479 |
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| author | Undine Proschmann Puya Shalchi Amirkhiz Pauline Andres Rocco Haase Hernan Inojosa Tjalf Ziemssen Katja Akgün |
| author_facet | Undine Proschmann Puya Shalchi Amirkhiz Pauline Andres Rocco Haase Hernan Inojosa Tjalf Ziemssen Katja Akgün |
| author_sort | Undine Proschmann |
| collection | DOAJ |
| description | Lymphocytes are key players in the pathogenesis of multiple sclerosis and a distinct target of several immunomodulatory treatment strategies. In this study, we aim to evaluate the effect of various pre-analytic conditions on immune cell counts to conclude the relevance for clinical implications. Twenty healthy donors were assessed for the effects of distinct storage temperatures and times after blood draws, different durations of tourniquet application, body positions and varying aspiration forces during blood draws. Immune cell frequencies were analyzed using multicolor flowcytometry. While storage for 24 h at 37 °C after blood draws was associated with significantly lower cell counts, different durations of tourniquet application, body positions and varying aspirations speeds did not have significant impacts on the immune cell counts. Our data suggest that immune cell counts are differently affected by pre-analytic conditions being more sensitive to storage temperature. Pre-analytic conditions should be carefully considered when interpreting the laboratory values of immune cell subpopulations. |
| first_indexed | 2024-03-10T23:21:56Z |
| format | Article |
| id | doaj.art-d5bbea7d3c9f4bfab595c51596f7de51 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:21:56Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d5bbea7d3c9f4bfab595c51596f7de512023-11-19T08:17:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124171347910.3390/ijms241713479Influence of Pre-Analytic Conditions on Quantity of LymphocytesUndine Proschmann0Puya Shalchi Amirkhiz1Pauline Andres2Rocco Haase3Hernan Inojosa4Tjalf Ziemssen5Katja Akgün6Center of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, GermanyCenter of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, GermanyCenter of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, GermanyCenter of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, GermanyCenter of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, GermanyCenter of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, GermanyCenter of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, GermanyLymphocytes are key players in the pathogenesis of multiple sclerosis and a distinct target of several immunomodulatory treatment strategies. In this study, we aim to evaluate the effect of various pre-analytic conditions on immune cell counts to conclude the relevance for clinical implications. Twenty healthy donors were assessed for the effects of distinct storage temperatures and times after blood draws, different durations of tourniquet application, body positions and varying aspiration forces during blood draws. Immune cell frequencies were analyzed using multicolor flowcytometry. While storage for 24 h at 37 °C after blood draws was associated with significantly lower cell counts, different durations of tourniquet application, body positions and varying aspirations speeds did not have significant impacts on the immune cell counts. Our data suggest that immune cell counts are differently affected by pre-analytic conditions being more sensitive to storage temperature. Pre-analytic conditions should be carefully considered when interpreting the laboratory values of immune cell subpopulations.https://www.mdpi.com/1422-0067/24/17/13479autoimmune diseasesflowcytometrylymphocytespre-analytic conditionsstorage temperaturestorage time |
| spellingShingle | Undine Proschmann Puya Shalchi Amirkhiz Pauline Andres Rocco Haase Hernan Inojosa Tjalf Ziemssen Katja Akgün Influence of Pre-Analytic Conditions on Quantity of Lymphocytes International Journal of Molecular Sciences autoimmune diseases flowcytometry lymphocytes pre-analytic conditions storage temperature storage time |
| title | Influence of Pre-Analytic Conditions on Quantity of Lymphocytes |
| title_full | Influence of Pre-Analytic Conditions on Quantity of Lymphocytes |
| title_fullStr | Influence of Pre-Analytic Conditions on Quantity of Lymphocytes |
| title_full_unstemmed | Influence of Pre-Analytic Conditions on Quantity of Lymphocytes |
| title_short | Influence of Pre-Analytic Conditions on Quantity of Lymphocytes |
| title_sort | influence of pre analytic conditions on quantity of lymphocytes |
| topic | autoimmune diseases flowcytometry lymphocytes pre-analytic conditions storage temperature storage time |
| url | https://www.mdpi.com/1422-0067/24/17/13479 |
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