Passive Immunity and Antibody Response Induced by <i>Toxoplasma gondii</i> VLP Immunization
Passive immunity can provide immediate protection against infectious pathogens. To date, only a few studies have investigated the effect of passive immunization against <i>Toxoplasma gondii</i>, and the use of immune sera acquired from VLP-vaccinated mice for passive immunity assessment...
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MDPI AG
2021-04-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/5/425 |
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| author | Hae-Ji Kang Min-Ju Kim Ki-Back Chu Su-Hwa Lee Eun-Kyung Moon Fu-Shi Quan |
| author_facet | Hae-Ji Kang Min-Ju Kim Ki-Back Chu Su-Hwa Lee Eun-Kyung Moon Fu-Shi Quan |
| author_sort | Hae-Ji Kang |
| collection | DOAJ |
| description | Passive immunity can provide immediate protection against infectious pathogens. To date, only a few studies have investigated the effect of passive immunization against <i>Toxoplasma gondii</i>, and the use of immune sera acquired from VLP-vaccinated mice for passive immunity assessment remains unreported. In this study, immune sera were produced by a single immunization with virus-like particles (VLPs) expressing the inner membrane complex (IMC), rhoptry protein 18 (ROP18), and microneme protein 8 (MIC8) of <i>Toxoplasma gondii</i>, with or without a CpG-ODN adjuvant. The passive immunization of immune sera conferred protection in mice, as indicated by their potent parasite-specific antibody response, lessened brain cyst counts, lower bodyweight loss, and enhanced survival. In order to confirm that the immune sera of the VLP-immunized mice were truly protective, the antibody responses and other immunological parameters were measured in the VLP-immunized mice. We found that VLP immunization induced higher levels of parasite-specific IgG, IgG subclass, and IgM antibody responses in the sera and intestines than in the controls. Enhanced Th1 and Th2-associated cytokines in the spleen, diminished brain cyst counts, and lessened body weight loss were found following <i>T. gondii</i> ME49 challenge infection. These results suggest that passive immunization with the immune sera acquired from VLP-vaccinated mice can confer adequate protection against <i>T. gondii</i> infection. |
| first_indexed | 2024-03-10T12:02:17Z |
| format | Article |
| id | doaj.art-d5bc74c72d5045228622a7170ffd7409 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T12:02:17Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-d5bc74c72d5045228622a7170ffd74092023-11-21T16:55:26ZengMDPI AGVaccines2076-393X2021-04-019542510.3390/vaccines9050425Passive Immunity and Antibody Response Induced by <i>Toxoplasma gondii</i> VLP ImmunizationHae-Ji Kang0Min-Ju Kim1Ki-Back Chu2Su-Hwa Lee3Eun-Kyung Moon4Fu-Shi Quan5Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, KoreaDepartment of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, KoreaDepartment of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, KoreaPassive immunity can provide immediate protection against infectious pathogens. To date, only a few studies have investigated the effect of passive immunization against <i>Toxoplasma gondii</i>, and the use of immune sera acquired from VLP-vaccinated mice for passive immunity assessment remains unreported. In this study, immune sera were produced by a single immunization with virus-like particles (VLPs) expressing the inner membrane complex (IMC), rhoptry protein 18 (ROP18), and microneme protein 8 (MIC8) of <i>Toxoplasma gondii</i>, with or without a CpG-ODN adjuvant. The passive immunization of immune sera conferred protection in mice, as indicated by their potent parasite-specific antibody response, lessened brain cyst counts, lower bodyweight loss, and enhanced survival. In order to confirm that the immune sera of the VLP-immunized mice were truly protective, the antibody responses and other immunological parameters were measured in the VLP-immunized mice. We found that VLP immunization induced higher levels of parasite-specific IgG, IgG subclass, and IgM antibody responses in the sera and intestines than in the controls. Enhanced Th1 and Th2-associated cytokines in the spleen, diminished brain cyst counts, and lessened body weight loss were found following <i>T. gondii</i> ME49 challenge infection. These results suggest that passive immunization with the immune sera acquired from VLP-vaccinated mice can confer adequate protection against <i>T. gondii</i> infection.https://www.mdpi.com/2076-393X/9/5/425<i>Toxoplasma gondii</i>virus-like particleCpG-ODNantibody responsecytokine |
| spellingShingle | Hae-Ji Kang Min-Ju Kim Ki-Back Chu Su-Hwa Lee Eun-Kyung Moon Fu-Shi Quan Passive Immunity and Antibody Response Induced by <i>Toxoplasma gondii</i> VLP Immunization Vaccines <i>Toxoplasma gondii</i> virus-like particle CpG-ODN antibody response cytokine |
| title | Passive Immunity and Antibody Response Induced by <i>Toxoplasma gondii</i> VLP Immunization |
| title_full | Passive Immunity and Antibody Response Induced by <i>Toxoplasma gondii</i> VLP Immunization |
| title_fullStr | Passive Immunity and Antibody Response Induced by <i>Toxoplasma gondii</i> VLP Immunization |
| title_full_unstemmed | Passive Immunity and Antibody Response Induced by <i>Toxoplasma gondii</i> VLP Immunization |
| title_short | Passive Immunity and Antibody Response Induced by <i>Toxoplasma gondii</i> VLP Immunization |
| title_sort | passive immunity and antibody response induced by i toxoplasma gondii i vlp immunization |
| topic | <i>Toxoplasma gondii</i> virus-like particle CpG-ODN antibody response cytokine |
| url | https://www.mdpi.com/2076-393X/9/5/425 |
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