Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation
Plant-based virus-like particle (VLP) vaccines have been studied for years, demonstrating their potential as antigen-presenting platforms. In this paper, we describe the development of, and compare between, simple <i>Escherichia coli</i>-based antigen display platforms for the generation...
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MDPI AG
2022-03-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/4/485 |
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| author | Anete Ogrina Dace Skrastina Ina Balke Ieva Kalnciema Juris Jansons Martin F. Bachmann Andris Zeltins |
| author_facet | Anete Ogrina Dace Skrastina Ina Balke Ieva Kalnciema Juris Jansons Martin F. Bachmann Andris Zeltins |
| author_sort | Anete Ogrina |
| collection | DOAJ |
| description | Plant-based virus-like particle (VLP) vaccines have been studied for years, demonstrating their potential as antigen-presenting platforms. In this paper, we describe the development of, and compare between, simple <i>Escherichia coli</i>-based antigen display platforms for the generation of potato virus Y (PVY) VLP-derived vaccines, thus allowing the production of vaccines from a single bacterial cell culture. We constructed four systems with the major cat allergen Fel d 1; namely, direct fusion with plant virus PVY coat protein (CP), mosaic PVY VLPs, and two coexpression variants of conjugates (SpyTag/SpyCatcher) allowing coexpression and conjugation directly in <i>E. coli</i> cells. For control experiments, we included PVY VLPs chemically coupled with Fel d 1. All constructed PVY–Fel d 1 variants were well expressed and soluble, formed PVY-like filamentous particles, and were recognized by monoclonal Fel d 1 antibodies. Our results indicate that all vaccine variants induced high titers of anti-Fel d 1 antibodies in murine models. Mice that were immunized with the chemically coupled Fel d 1 antigen exhibited the highest antibody titers and antibody–antigen interaction specificity, as detected by binding avidity and recognition of native Fel d 1. IgG1 subclass antibodies were found to be the dominant IgG class against PVY–Fel d 1. PVY CP-derived VLPs represent an efficient platform for the comparison of various antigen presentation systems to help evaluate different vaccine designs. |
| first_indexed | 2024-03-09T04:09:05Z |
| format | Article |
| id | doaj.art-d5bd9c9df3f8453b8c394cc5bef41de3 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T04:09:05Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-d5bd9c9df3f8453b8c394cc5bef41de32023-12-03T14:02:31ZengMDPI AGVaccines2076-393X2022-03-0110448510.3390/vaccines10040485Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine GenerationAnete Ogrina0Dace Skrastina1Ina Balke2Ieva Kalnciema3Juris Jansons4Martin F. Bachmann5Andris Zeltins6Plant Virology Laboratory, Latvian Biomedical Research and Study Centre, LV-1067 Riga, LatviaPlant Virology Laboratory, Latvian Biomedical Research and Study Centre, LV-1067 Riga, LatviaPlant Virology Laboratory, Latvian Biomedical Research and Study Centre, LV-1067 Riga, LatviaPlant Virology Laboratory, Latvian Biomedical Research and Study Centre, LV-1067 Riga, LatviaPlant Virology Laboratory, Latvian Biomedical Research and Study Centre, LV-1067 Riga, LatviaDepartment of BioMedical Research, University of Bern, 3008 Bern, SwitzerlandPlant Virology Laboratory, Latvian Biomedical Research and Study Centre, LV-1067 Riga, LatviaPlant-based virus-like particle (VLP) vaccines have been studied for years, demonstrating their potential as antigen-presenting platforms. In this paper, we describe the development of, and compare between, simple <i>Escherichia coli</i>-based antigen display platforms for the generation of potato virus Y (PVY) VLP-derived vaccines, thus allowing the production of vaccines from a single bacterial cell culture. We constructed four systems with the major cat allergen Fel d 1; namely, direct fusion with plant virus PVY coat protein (CP), mosaic PVY VLPs, and two coexpression variants of conjugates (SpyTag/SpyCatcher) allowing coexpression and conjugation directly in <i>E. coli</i> cells. For control experiments, we included PVY VLPs chemically coupled with Fel d 1. All constructed PVY–Fel d 1 variants were well expressed and soluble, formed PVY-like filamentous particles, and were recognized by monoclonal Fel d 1 antibodies. Our results indicate that all vaccine variants induced high titers of anti-Fel d 1 antibodies in murine models. Mice that were immunized with the chemically coupled Fel d 1 antigen exhibited the highest antibody titers and antibody–antigen interaction specificity, as detected by binding avidity and recognition of native Fel d 1. IgG1 subclass antibodies were found to be the dominant IgG class against PVY–Fel d 1. PVY CP-derived VLPs represent an efficient platform for the comparison of various antigen presentation systems to help evaluate different vaccine designs.https://www.mdpi.com/2076-393X/10/4/485virus-like particles<i>E. coli</i>expressionpotato virus YFel d 1 |
| spellingShingle | Anete Ogrina Dace Skrastina Ina Balke Ieva Kalnciema Juris Jansons Martin F. Bachmann Andris Zeltins Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation Vaccines virus-like particles <i>E. coli</i> expression potato virus Y Fel d 1 |
| title | Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation |
| title_full | Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation |
| title_fullStr | Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation |
| title_full_unstemmed | Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation |
| title_short | Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation |
| title_sort | comparison of bacterial expression systems based on potato virus y like particles for vaccine generation |
| topic | virus-like particles <i>E. coli</i> expression potato virus Y Fel d 1 |
| url | https://www.mdpi.com/2076-393X/10/4/485 |
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