Differentiation Induction Effect of Mir-429 Over-Expression in U251 Glioma Cell Line
The purpose of this research was to investigate the effect of mir-429 ectopic over-expression on U251 glioblastoma cell line. It has been shown that mir-429 could target BMK1 and reduce glioma cells invasion. We cultured U251-mir-429 cells and observed changes in their morphology for 14 days. They s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Shahid Beheshti University of Medical Sciences
2016-10-01
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| Series: | International Clinical Neuroscience Journal |
| Online Access: | http://journals.sbmu.ac.ir/Neuroscience/article/download/15756/5 |
| _version_ | 1811220629135818752 |
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| author | Fatemeh Gheidari Ehsan Arefian Fatemeh Jamshidi-Adeghani Masoud Soleimani |
| author_facet | Fatemeh Gheidari Ehsan Arefian Fatemeh Jamshidi-Adeghani Masoud Soleimani |
| author_sort | Fatemeh Gheidari |
| collection | DOAJ |
| description | The purpose of this research was to investigate the effect of mir-429 ectopic over-expression on U251 glioblastoma cell line. It has been shown that mir-429 could target BMK1 and reduce glioma cells invasion. We cultured U251-mir-429 cells and observed changes in their morphology for 14 days. They seemed to become wider with expanded cytoplasm and go on differentiation. Neuronal (NEFM, NSE and neurogenin) and oligodendrocyte (MBP, OLIG2 and NKX2-2) gene markers expression level was examined with real-time PCR. NSE, neurogenin, MBP, OLIG2 and NKX2-2 were significantly over-expressed, giving an evidence of cellular differentiation in glioma cells. |
| first_indexed | 2024-04-12T07:44:56Z |
| format | Article |
| id | doaj.art-d5bfed20606f46cb89c22ee02d2c8f7d |
| institution | Directory Open Access Journal |
| issn | 2383-1871 2383-2096 |
| language | English |
| last_indexed | 2024-04-12T07:44:56Z |
| publishDate | 2016-10-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | International Clinical Neuroscience Journal |
| spelling | doaj.art-d5bfed20606f46cb89c22ee02d2c8f7d2022-12-22T03:41:43ZengShahid Beheshti University of Medical SciencesInternational Clinical Neuroscience Journal2383-18712383-20962016-10-013420120510.22037/icnj.v3i4.15756icnj-3092Differentiation Induction Effect of Mir-429 Over-Expression in U251 Glioma Cell LineFatemeh Gheidari0Ehsan Arefian1Fatemeh Jamshidi-Adeghani2Masoud Soleimani3Department of Biotechnology, University of Tehran, Tehran, IranSchool of Biology, College of Science, University of Tehran, Tehran, IranStem Cell Technology Research Center, Tehran, IranDepartment of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, IranThe purpose of this research was to investigate the effect of mir-429 ectopic over-expression on U251 glioblastoma cell line. It has been shown that mir-429 could target BMK1 and reduce glioma cells invasion. We cultured U251-mir-429 cells and observed changes in their morphology for 14 days. They seemed to become wider with expanded cytoplasm and go on differentiation. Neuronal (NEFM, NSE and neurogenin) and oligodendrocyte (MBP, OLIG2 and NKX2-2) gene markers expression level was examined with real-time PCR. NSE, neurogenin, MBP, OLIG2 and NKX2-2 were significantly over-expressed, giving an evidence of cellular differentiation in glioma cells.http://journals.sbmu.ac.ir/Neuroscience/article/download/15756/5 |
| spellingShingle | Fatemeh Gheidari Ehsan Arefian Fatemeh Jamshidi-Adeghani Masoud Soleimani Differentiation Induction Effect of Mir-429 Over-Expression in U251 Glioma Cell Line International Clinical Neuroscience Journal |
| title | Differentiation Induction Effect of Mir-429 Over-Expression in U251 Glioma Cell Line |
| title_full | Differentiation Induction Effect of Mir-429 Over-Expression in U251 Glioma Cell Line |
| title_fullStr | Differentiation Induction Effect of Mir-429 Over-Expression in U251 Glioma Cell Line |
| title_full_unstemmed | Differentiation Induction Effect of Mir-429 Over-Expression in U251 Glioma Cell Line |
| title_short | Differentiation Induction Effect of Mir-429 Over-Expression in U251 Glioma Cell Line |
| title_sort | differentiation induction effect of mir 429 over expression in u251 glioma cell line |
| url | http://journals.sbmu.ac.ir/Neuroscience/article/download/15756/5 |
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