Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida
Mottle disease of carnation (Dianthus caryophyllus) was observed in cultivation areas in Cianjur and Bandung, West Java. This study aimed to identify the virus causing leaf mottling on carnation using reverse transcription-polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis....
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Indonesian |
| Published: |
Perhimpunan Fitopatologi Indonesia
2014-09-01
|
| Series: | Jurnal Fitopatologi Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jfiti/article/view/8580 |
| _version_ | 1828072734275403776 |
|---|---|
| author | Rizki Haerunisa Erniawati Diningsih Gede Suastika |
| author_facet | Rizki Haerunisa Erniawati Diningsih Gede Suastika |
| author_sort | Rizki Haerunisa |
| collection | DOAJ |
| description |
Mottle disease of carnation (Dianthus caryophyllus) was observed in cultivation areas in Cianjur and Bandung, West Java. This study aimed to identify the virus causing leaf mottling on carnation using reverse transcription-polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. Fragment DNA of 1000 bp was successfully amplified using specific primers for coat protein of Carmovirus (BC57/BC58). Direct sequencing was proceeded using PCR products. Nucleotide sequence analysis confirmed the infection of Carnation mottle virus (CarMV) from leaves showing mottle symptom. Furthermore, phylogenetic analysis showed that CarMV isolates from Indonesia have close relationship with those from Brazil, Israel, Iran, India, Netherland, and Spain.
|
| first_indexed | 2024-04-11T01:16:43Z |
| format | Article |
| id | doaj.art-d5c0325d32af45d4bcbe4075a22f048f |
| institution | Directory Open Access Journal |
| issn | 0215-7950 2339-2479 |
| language | Indonesian |
| last_indexed | 2024-04-11T01:16:43Z |
| publishDate | 2014-09-01 |
| publisher | Perhimpunan Fitopatologi Indonesia |
| record_format | Article |
| series | Jurnal Fitopatologi Indonesia |
| spelling | doaj.art-d5c0325d32af45d4bcbe4075a22f048f2023-01-04T02:44:52ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792014-09-0110310.14692/jfi.10.3.877244Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen NukleotidaRizki Haerunisa0Erniawati Diningsih1Gede Suastika2Institut Pertanian BogorInstitut Pertanian BogorInstitut Pertanian Bogor Mottle disease of carnation (Dianthus caryophyllus) was observed in cultivation areas in Cianjur and Bandung, West Java. This study aimed to identify the virus causing leaf mottling on carnation using reverse transcription-polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. Fragment DNA of 1000 bp was successfully amplified using specific primers for coat protein of Carmovirus (BC57/BC58). Direct sequencing was proceeded using PCR products. Nucleotide sequence analysis confirmed the infection of Carnation mottle virus (CarMV) from leaves showing mottle symptom. Furthermore, phylogenetic analysis showed that CarMV isolates from Indonesia have close relationship with those from Brazil, Israel, Iran, India, Netherland, and Spain. https://journal.ipb.ac.id/index.php/jfiti/article/view/8580 |
| spellingShingle | Rizki Haerunisa Erniawati Diningsih Gede Suastika Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida Jurnal Fitopatologi Indonesia |
| title | Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
| title_full | Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
| title_fullStr | Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
| title_full_unstemmed | Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
| title_short | Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
| title_sort | identifikasi carmovirus pada tanaman anyelir melalui teknik reverse transcription polymerase chain reaction dan analisis sikuen nukleotida |
| url | https://journal.ipb.ac.id/index.php/jfiti/article/view/8580 |
| work_keys_str_mv | AT rizkihaerunisa identifikasicarmoviruspadatanamananyelirmelaluiteknikreversetranscriptionpolymerasechainreactiondananalisissikuennukleotida AT erniawatidiningsih identifikasicarmoviruspadatanamananyelirmelaluiteknikreversetranscriptionpolymerasechainreactiondananalisissikuennukleotida AT gedesuastika identifikasicarmoviruspadatanamananyelirmelaluiteknikreversetranscriptionpolymerasechainreactiondananalisissikuennukleotida |