Astragalus protects PC12 cells from 6-hydroxydopamine-induced neuronal damage: A serum pharmacological study
Accumulating evidence has already indicated that traditional Chinese medicine (TCM) possesses tremendous potential for treating neurodegenerative diseases. Astragalus, also named Huangqi, is a famous traditional medical herb that can be applied to treat cerebral ischemia and prevent neuronal degener...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Chinese Journal of Physiology |
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| Online Access: | http://www.cjphysiology.org/article.asp?issn=0304-4920;year=2021;volume=64;issue=1;spage=24;epage=31;aulast=Guo |
| _version_ | 1831553067645927424 |
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| author | Li-Ying Guo Feng-Lei Shi Meng Li Jin-Hao Sun Chuan-Gang Li Zeng-Xun Liu |
| author_facet | Li-Ying Guo Feng-Lei Shi Meng Li Jin-Hao Sun Chuan-Gang Li Zeng-Xun Liu |
| author_sort | Li-Ying Guo |
| collection | DOAJ |
| description | Accumulating evidence has already indicated that traditional Chinese medicine (TCM) possesses tremendous potential for treating neurodegenerative diseases. Astragalus, also named Huangqi, is a famous traditional medical herb that can be applied to treat cerebral ischemia and prevent neuronal degeneration. Nevertheless, the underlying mechanisms remain largely unexplored. In the present study, Astragalus-containing serum (ASMES) was prepared and added into the culture medium of PC12 cells to explore its neuroprotective effect on 6-hydroxydopamine (6-OHDA)-caused neuronal toxicity. Our data showed that ASMES significantly ameliorated the cellular viability of cultured PC12 cells against the neurotoxicity induced by 6-OHDA (P < 0.05). Moreover, ASMES significantly decreased the cell apoptosis triggered by 6-OHDA (P < 0.01). Furthermore, 2′,7′-dichlorofluorescin diacetate assay was performed to detect the changes in oxidative stress, and we showed that 6-OHDA elevated the production of reactive oxygen species (ROS), whereas ASMES significantly reversed these changes (P < 0.01). Besides, mitochondrial membrane potential (MMP) assay showed that ASMES could restore 6-OHDA-damaged MMP in cultured PC12 cells (P < 0.05). In conclusion, Astragalus could protect PC12 cells from 6-OHDA-caused neuronal toxicity, and possibly, the ROS-mediated apoptotic pathway participated in this process. Collectively, our findings provided valuable insights into the potential in treatment of neurodegenerative diseases. |
| first_indexed | 2024-12-17T03:38:22Z |
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| id | doaj.art-d5c28f7cd1014493997f9eef12f94ad0 |
| institution | Directory Open Access Journal |
| issn | 0304-4920 2666-0059 |
| language | English |
| last_indexed | 2024-12-17T03:38:22Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Chinese Journal of Physiology |
| spelling | doaj.art-d5c28f7cd1014493997f9eef12f94ad02022-12-21T22:05:04ZengWolters Kluwer Medknow PublicationsChinese Journal of Physiology0304-49202666-00592021-01-01641243110.4103/CJP.CJP_50_20Astragalus protects PC12 cells from 6-hydroxydopamine-induced neuronal damage: A serum pharmacological studyLi-Ying GuoFeng-Lei ShiMeng LiJin-Hao SunChuan-Gang LiZeng-Xun LiuAccumulating evidence has already indicated that traditional Chinese medicine (TCM) possesses tremendous potential for treating neurodegenerative diseases. Astragalus, also named Huangqi, is a famous traditional medical herb that can be applied to treat cerebral ischemia and prevent neuronal degeneration. Nevertheless, the underlying mechanisms remain largely unexplored. In the present study, Astragalus-containing serum (ASMES) was prepared and added into the culture medium of PC12 cells to explore its neuroprotective effect on 6-hydroxydopamine (6-OHDA)-caused neuronal toxicity. Our data showed that ASMES significantly ameliorated the cellular viability of cultured PC12 cells against the neurotoxicity induced by 6-OHDA (P < 0.05). Moreover, ASMES significantly decreased the cell apoptosis triggered by 6-OHDA (P < 0.01). Furthermore, 2′,7′-dichlorofluorescin diacetate assay was performed to detect the changes in oxidative stress, and we showed that 6-OHDA elevated the production of reactive oxygen species (ROS), whereas ASMES significantly reversed these changes (P < 0.01). Besides, mitochondrial membrane potential (MMP) assay showed that ASMES could restore 6-OHDA-damaged MMP in cultured PC12 cells (P < 0.05). In conclusion, Astragalus could protect PC12 cells from 6-OHDA-caused neuronal toxicity, and possibly, the ROS-mediated apoptotic pathway participated in this process. Collectively, our findings provided valuable insights into the potential in treatment of neurodegenerative diseases.http://www.cjphysiology.org/article.asp?issn=0304-4920;year=2021;volume=64;issue=1;spage=24;epage=31;aulast=Guo6-hydroxydopamineapoptosisastragalusneuroprotectionserum pharmacology |
| spellingShingle | Li-Ying Guo Feng-Lei Shi Meng Li Jin-Hao Sun Chuan-Gang Li Zeng-Xun Liu Astragalus protects PC12 cells from 6-hydroxydopamine-induced neuronal damage: A serum pharmacological study Chinese Journal of Physiology 6-hydroxydopamine apoptosis astragalus neuroprotection serum pharmacology |
| title | Astragalus protects PC12 cells from 6-hydroxydopamine-induced neuronal damage: A serum pharmacological study |
| title_full | Astragalus protects PC12 cells from 6-hydroxydopamine-induced neuronal damage: A serum pharmacological study |
| title_fullStr | Astragalus protects PC12 cells from 6-hydroxydopamine-induced neuronal damage: A serum pharmacological study |
| title_full_unstemmed | Astragalus protects PC12 cells from 6-hydroxydopamine-induced neuronal damage: A serum pharmacological study |
| title_short | Astragalus protects PC12 cells from 6-hydroxydopamine-induced neuronal damage: A serum pharmacological study |
| title_sort | astragalus protects pc12 cells from 6 hydroxydopamine induced neuronal damage a serum pharmacological study |
| topic | 6-hydroxydopamine apoptosis astragalus neuroprotection serum pharmacology |
| url | http://www.cjphysiology.org/article.asp?issn=0304-4920;year=2021;volume=64;issue=1;spage=24;epage=31;aulast=Guo |
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