PROPERTIES OF ISOLATED NEURAL CELLS FROM NEWBORN RAT IN TISSUE in vitro
It is shown that isolated brain cells of newborn rats are heterogeneous suspension consisted of differentiated cells, stem and progenitor cells, which can proliferate and differentiate in conditions of in vitro cultivation. During cultivation in presence of serum the neural cells of the newborn rats...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2013-06-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/no3_2013/sukach_3_2013.pdf |
| _version_ | 1827848538529202176 |
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| author | A. N. Sukach T. D. Liashenko M. V. Shevchenko |
| author_facet | A. N. Sukach T. D. Liashenko M. V. Shevchenko |
| author_sort | A. N. Sukach |
| collection | DOAJ |
| description | It is shown that isolated brain cells of newborn rats are heterogeneous suspension consisted of differentiated cells, stem and progenitor cells, which can proliferate and differentiate in conditions of in vitro cultivation. During cultivation in presence of serum the neural cells of the newborn rats form aggregates, where reconstitution of cellular microenvironment lost in isolation takes place followed by cell reparation. After attachment to a substrate the aggregate cells migrate and spread, form a monolayer of astroglia, which the neuroblasts and colonies of stem/progenitor cells are formed on. The findings show feasible application of newborn rat neural cells as model of postnatal neural tissue for studying the influence of cellular environment on process of development and recovery of differentiated neural cells, as well as proliferation and differentiation of stem/progenitor cells. |
| first_indexed | 2024-03-12T09:48:34Z |
| format | Article |
| id | doaj.art-d5c388903cc3454fbf382720aa860f1f |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T09:48:34Z |
| publishDate | 2013-06-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-d5c388903cc3454fbf382720aa860f1f2023-09-02T12:44:32ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2013-06-0163636810.15407/biotech6.03.063PROPERTIES OF ISOLATED NEURAL CELLS FROM NEWBORN RAT IN TISSUE in vitroA. N. Sukach0T. D. Liashenko1M. V. Shevchenko2Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv; Skovoroda National Pedagogical University, Kharkiv, UkraineSkovoroda National Pedagogical University, Kharkiv, UkraineSkovoroda National Pedagogical University, Kharkiv, UkraineIt is shown that isolated brain cells of newborn rats are heterogeneous suspension consisted of differentiated cells, stem and progenitor cells, which can proliferate and differentiate in conditions of in vitro cultivation. During cultivation in presence of serum the neural cells of the newborn rats form aggregates, where reconstitution of cellular microenvironment lost in isolation takes place followed by cell reparation. After attachment to a substrate the aggregate cells migrate and spread, form a monolayer of astroglia, which the neuroblasts and colonies of stem/progenitor cells are formed on. The findings show feasible application of newborn rat neural cells as model of postnatal neural tissue for studying the influence of cellular environment on process of development and recovery of differentiated neural cells, as well as proliferation and differentiation of stem/progenitor cells.http://biotechnology.kiev.ua/images/storage/no3_2013/sukach_3_2013.pdfdifferentiated nervous cellsnervous stem/progenitor cellsnew-born ratsviabilitysurvivalcultivationmulticellular aggregates |
| spellingShingle | A. N. Sukach T. D. Liashenko M. V. Shevchenko PROPERTIES OF ISOLATED NEURAL CELLS FROM NEWBORN RAT IN TISSUE in vitro Biotechnologia Acta differentiated nervous cells nervous stem/progenitor cells new-born rats viability survival cultivation multicellular aggregates |
| title | PROPERTIES OF ISOLATED NEURAL CELLS FROM NEWBORN RAT IN TISSUE in vitro |
| title_full | PROPERTIES OF ISOLATED NEURAL CELLS FROM NEWBORN RAT IN TISSUE in vitro |
| title_fullStr | PROPERTIES OF ISOLATED NEURAL CELLS FROM NEWBORN RAT IN TISSUE in vitro |
| title_full_unstemmed | PROPERTIES OF ISOLATED NEURAL CELLS FROM NEWBORN RAT IN TISSUE in vitro |
| title_short | PROPERTIES OF ISOLATED NEURAL CELLS FROM NEWBORN RAT IN TISSUE in vitro |
| title_sort | properties of isolated neural cells from newborn rat in tissue in vitro |
| topic | differentiated nervous cells nervous stem/progenitor cells new-born rats viability survival cultivation multicellular aggregates |
| url | http://biotechnology.kiev.ua/images/storage/no3_2013/sukach_3_2013.pdf |
| work_keys_str_mv | AT ansukach propertiesofisolatedneuralcellsfromnewbornratintissueinvitro AT tdliashenko propertiesofisolatedneuralcellsfromnewbornratintissueinvitro AT mvshevchenko propertiesofisolatedneuralcellsfromnewbornratintissueinvitro |