Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface
Microbial communities play a key role for central biogeochemical cycles in the subsurface. Little is known about whether short-term seasonal drought and rewetting events influence the dominant microbes involved in C- and N-cycles. Here, we applied metaproteomics at different subsurface sites in wint...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/11/1694 |
| _version_ | 1797549277024616448 |
|---|---|
| author | Patrick Lohmann Simon Benk Gerd Gleixner Karin Potthast Beate Michalzik Nico Jehmlich Martin von Bergen |
| author_facet | Patrick Lohmann Simon Benk Gerd Gleixner Karin Potthast Beate Michalzik Nico Jehmlich Martin von Bergen |
| author_sort | Patrick Lohmann |
| collection | DOAJ |
| description | Microbial communities play a key role for central biogeochemical cycles in the subsurface. Little is known about whether short-term seasonal drought and rewetting events influence the dominant microbes involved in C- and N-cycles. Here, we applied metaproteomics at different subsurface sites in winter, summer and autumn from surface litter layer, seepage water at increasing subsoil depths and remote located groundwater from two wells within the Hainich Critical Zone Exploratory, Germany. We observed changes in the dominance of microbial families at subsurface sampling sites with increasing distances, i.e., <i>Microcoleaceae</i> dominated in topsoil seepage, while <i>Candidatus Brocadiaceae</i> dominated at deeper and more distant groundwater wells. Nitrifying bacteria showed a shift in dominance from drought to rewetting events from summer by <i>Nitrosomandaceae</i> to autumn by <i>Candidatus Brocadiaceae</i>. We further observed that the reductive pentose phosphate pathway was a prominent CO<sub>2</sub>-fixation strategy, dominated by <i>Woeseiaceae</i> in wet early winter, which decreased under drought conditions and changed to a dominance of <i>Sphingobacteriaceae</i> under rewetting conditions. This study shows that increasing subsurface sites and rewetting event after drought alter the dominances of key subsurface microbes. This helps to predict the consequences of annual seasonal dynamics on the nutrient cycling microbes that contribute to ecosystem functioning. |
| first_indexed | 2024-03-10T15:12:23Z |
| format | Article |
| id | doaj.art-d5c61023935e4e2fbf3716f0416af239 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:12:23Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d5c61023935e4e2fbf3716f0416af2392023-11-20T19:14:19ZengMDPI AGMicroorganisms2076-26072020-10-01811169410.3390/microorganisms8111694Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the SubsurfacePatrick Lohmann0Simon Benk1Gerd Gleixner2Karin Potthast3Beate Michalzik4Nico Jehmlich5Martin von Bergen6Department of Molecular Systems Biology, Helmholtz-Centre for Environmental Research GmbH—UFZ, 04318 Leipzig, GermanyDepartment of Molecular Biogeochemistry, Max-Planck-Institute for Biogeochemistry, 07745 Jena, GermanyDepartment of Molecular Biogeochemistry, Max-Planck-Institute for Biogeochemistry, 07745 Jena, GermanyDepartment of Soil Science, Friedrich Schiller University, 07743 Jena, GermanyDepartment of Soil Science, Friedrich Schiller University, 07743 Jena, GermanyDepartment of Molecular Systems Biology, Helmholtz-Centre for Environmental Research GmbH—UFZ, 04318 Leipzig, GermanyDepartment of Molecular Systems Biology, Helmholtz-Centre for Environmental Research GmbH—UFZ, 04318 Leipzig, GermanyMicrobial communities play a key role for central biogeochemical cycles in the subsurface. Little is known about whether short-term seasonal drought and rewetting events influence the dominant microbes involved in C- and N-cycles. Here, we applied metaproteomics at different subsurface sites in winter, summer and autumn from surface litter layer, seepage water at increasing subsoil depths and remote located groundwater from two wells within the Hainich Critical Zone Exploratory, Germany. We observed changes in the dominance of microbial families at subsurface sampling sites with increasing distances, i.e., <i>Microcoleaceae</i> dominated in topsoil seepage, while <i>Candidatus Brocadiaceae</i> dominated at deeper and more distant groundwater wells. Nitrifying bacteria showed a shift in dominance from drought to rewetting events from summer by <i>Nitrosomandaceae</i> to autumn by <i>Candidatus Brocadiaceae</i>. We further observed that the reductive pentose phosphate pathway was a prominent CO<sub>2</sub>-fixation strategy, dominated by <i>Woeseiaceae</i> in wet early winter, which decreased under drought conditions and changed to a dominance of <i>Sphingobacteriaceae</i> under rewetting conditions. This study shows that increasing subsurface sites and rewetting event after drought alter the dominances of key subsurface microbes. This helps to predict the consequences of annual seasonal dynamics on the nutrient cycling microbes that contribute to ecosystem functioning.https://www.mdpi.com/2076-2607/8/11/1694metaproteomicsmicrobial communitiessubsurfacenutrient cyclescritical zone |
| spellingShingle | Patrick Lohmann Simon Benk Gerd Gleixner Karin Potthast Beate Michalzik Nico Jehmlich Martin von Bergen Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface Microorganisms metaproteomics microbial communities subsurface nutrient cycles critical zone |
| title | Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface |
| title_full | Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface |
| title_fullStr | Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface |
| title_full_unstemmed | Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface |
| title_short | Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface |
| title_sort | seasonal patterns of dominant microbes involved in central nutrient cycles in the subsurface |
| topic | metaproteomics microbial communities subsurface nutrient cycles critical zone |
| url | https://www.mdpi.com/2076-2607/8/11/1694 |
| work_keys_str_mv | AT patricklohmann seasonalpatternsofdominantmicrobesinvolvedincentralnutrientcyclesinthesubsurface AT simonbenk seasonalpatternsofdominantmicrobesinvolvedincentralnutrientcyclesinthesubsurface AT gerdgleixner seasonalpatternsofdominantmicrobesinvolvedincentralnutrientcyclesinthesubsurface AT karinpotthast seasonalpatternsofdominantmicrobesinvolvedincentralnutrientcyclesinthesubsurface AT beatemichalzik seasonalpatternsofdominantmicrobesinvolvedincentralnutrientcyclesinthesubsurface AT nicojehmlich seasonalpatternsofdominantmicrobesinvolvedincentralnutrientcyclesinthesubsurface AT martinvonbergen seasonalpatternsofdominantmicrobesinvolvedincentralnutrientcyclesinthesubsurface |