Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes
Raman spectroscopy is one of the most used biodetection techniques. However, its usability is hampered in the case of low concentrated substances because of the weak intensity of the Raman signal. To overcome this limitation, the use of drop coating deposition Raman spectroscopy (DCDRS), in which th...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/13/22/4023 |
| _version_ | 1797508687094349824 |
|---|---|
| author | Alžbeta Kuižová Anna Kuzminova Ondřej Kylián Eva Kočišová |
| author_facet | Alžbeta Kuižová Anna Kuzminova Ondřej Kylián Eva Kočišová |
| author_sort | Alžbeta Kuižová |
| collection | DOAJ |
| description | Raman spectroscopy is one of the most used biodetection techniques. However, its usability is hampered in the case of low concentrated substances because of the weak intensity of the Raman signal. To overcome this limitation, the use of drop coating deposition Raman spectroscopy (DCDRS), in which the liquid samples are allowed to dry into well-defined patterns where the non-volatile solutes are highly concentrated, is appropriate. This significantly improves the Raman sensitivity when compared to the conventional Raman signal from solution/suspension. As DCDRS performance strongly depends on the wetting properties of substrates, we demonstrate here that the smooth hydrophobic plasma polymerized fluorocarbon films prepared by magnetron sputtering (contact angle 108°) are well-suited for the DCDRS detection of liposomes. Furthermore, it was proved that even better improvement of the Raman signal might be achieved if the plasma polymer surfaces are roughened. In this case, 100% higher intensities of Raman signal are observed in comparison with smooth fluorocarbon films. As it is shown, this effect, which has no influence on the profile of Raman spectra, is connected with the increased hydrophobicity of nanostructured fluorocarbon films. This results in the formation of dried liposomal deposits with smaller diameters and higher preconcentration of liposomes. |
| first_indexed | 2024-03-10T05:07:25Z |
| format | Article |
| id | doaj.art-d5c740977aaa43d1aa8741fef615bb35 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T05:07:25Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-d5c740977aaa43d1aa8741fef615bb352023-11-23T01:10:37ZengMDPI AGPolymers2073-43602021-11-011322402310.3390/polym13224023Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of LiposomesAlžbeta Kuižová0Anna Kuzminova1Ondřej Kylián2Eva Kočišová3Faculty of Mathematics and Physics, Institute of Physics, Charles University, Ke Karlovu 5, 121 16 Prague 2, Czech RepublicDepartment of Macromolecular Physics, Faculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 180 00 Prague 8, Czech RepublicDepartment of Macromolecular Physics, Faculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 180 00 Prague 8, Czech RepublicFaculty of Mathematics and Physics, Institute of Physics, Charles University, Ke Karlovu 5, 121 16 Prague 2, Czech RepublicRaman spectroscopy is one of the most used biodetection techniques. However, its usability is hampered in the case of low concentrated substances because of the weak intensity of the Raman signal. To overcome this limitation, the use of drop coating deposition Raman spectroscopy (DCDRS), in which the liquid samples are allowed to dry into well-defined patterns where the non-volatile solutes are highly concentrated, is appropriate. This significantly improves the Raman sensitivity when compared to the conventional Raman signal from solution/suspension. As DCDRS performance strongly depends on the wetting properties of substrates, we demonstrate here that the smooth hydrophobic plasma polymerized fluorocarbon films prepared by magnetron sputtering (contact angle 108°) are well-suited for the DCDRS detection of liposomes. Furthermore, it was proved that even better improvement of the Raman signal might be achieved if the plasma polymer surfaces are roughened. In this case, 100% higher intensities of Raman signal are observed in comparison with smooth fluorocarbon films. As it is shown, this effect, which has no influence on the profile of Raman spectra, is connected with the increased hydrophobicity of nanostructured fluorocarbon films. This results in the formation of dried liposomal deposits with smaller diameters and higher preconcentration of liposomes.https://www.mdpi.com/2073-4360/13/22/4023drop coating deposition Raman spectroscopyDCDRSplasma polymersliposomesnanostructured surfaceswettability |
| spellingShingle | Alžbeta Kuižová Anna Kuzminova Ondřej Kylián Eva Kočišová Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes Polymers drop coating deposition Raman spectroscopy DCDRS plasma polymers liposomes nanostructured surfaces wettability |
| title | Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes |
| title_full | Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes |
| title_fullStr | Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes |
| title_full_unstemmed | Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes |
| title_short | Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes |
| title_sort | nanostructured plasma polymerized fluorocarbon films for drop coating deposition raman spectroscopy dcdrs of liposomes |
| topic | drop coating deposition Raman spectroscopy DCDRS plasma polymers liposomes nanostructured surfaces wettability |
| url | https://www.mdpi.com/2073-4360/13/22/4023 |
| work_keys_str_mv | AT alzbetakuizova nanostructuredplasmapolymerizedfluorocarbonfilmsfordropcoatingdepositionramanspectroscopydcdrsofliposomes AT annakuzminova nanostructuredplasmapolymerizedfluorocarbonfilmsfordropcoatingdepositionramanspectroscopydcdrsofliposomes AT ondrejkylian nanostructuredplasmapolymerizedfluorocarbonfilmsfordropcoatingdepositionramanspectroscopydcdrsofliposomes AT evakocisova nanostructuredplasmapolymerizedfluorocarbonfilmsfordropcoatingdepositionramanspectroscopydcdrsofliposomes |