Ascorbate-deficient vtc2 mutants in Arabidopsis do not exhibit decreased growth
In higher plants the L-galactose pathway represents the major route for ascorbate biosynthesis. The first committed step of this pathway is catalysed by the enzyme GDP-L-galactose phosphorylase and is encoded by two paralogs in Arabidopsis – VITAMIN C2 (VTC2) and VTC5. The first mutant of this enzy...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-07-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01025/full |
| _version_ | 1818521920449019904 |
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| author | Benson Lim Nicholas Smirnoff Christopher S Cobbett John F Golz |
| author_facet | Benson Lim Nicholas Smirnoff Christopher S Cobbett John F Golz |
| author_sort | Benson Lim |
| collection | DOAJ |
| description | In higher plants the L-galactose pathway represents the major route for ascorbate biosynthesis. The first committed step of this pathway is catalysed by the enzyme GDP-L-galactose phosphorylase and is encoded by two paralogs in Arabidopsis – VITAMIN C2 (VTC2) and VTC5. The first mutant of this enzyme, vtc2-1, isolated via an EMS mutagenesis screen, has approximately 20-30% of wildtype ascorbate levels and has been reported to have decreased growth under standard laboratory conditions. Here, we show that a T-DNA insertion into the VTC2 causes a similar reduction in ascorbate levels, but does not greatly affect plant growth. Subsequent segregation analysis revealed the growth defects of vtc2-1 mutants segregate independently of the vtc2-1 mutation. These observations suggest the presence of an independent cryptic mutation that affects growth in vtc2-1 mutants and that a 70-80% decrease in ascorbate is not sufficient to a dramatically alter plant growth as has been assumed in past studies. |
| first_indexed | 2024-12-11T01:57:49Z |
| format | Article |
| id | doaj.art-d5cb0133a1bc4ebfa561ee381ec63416 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T01:57:49Z |
| publishDate | 2016-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d5cb0133a1bc4ebfa561ee381ec634162022-12-22T01:24:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-07-01710.3389/fpls.2016.01025205811Ascorbate-deficient vtc2 mutants in Arabidopsis do not exhibit decreased growthBenson Lim0Nicholas Smirnoff1Christopher S Cobbett2John F Golz3University of MelbourneUniversity of ExeterUniversity of MelbourneUniversity of MelbourneIn higher plants the L-galactose pathway represents the major route for ascorbate biosynthesis. The first committed step of this pathway is catalysed by the enzyme GDP-L-galactose phosphorylase and is encoded by two paralogs in Arabidopsis – VITAMIN C2 (VTC2) and VTC5. The first mutant of this enzyme, vtc2-1, isolated via an EMS mutagenesis screen, has approximately 20-30% of wildtype ascorbate levels and has been reported to have decreased growth under standard laboratory conditions. Here, we show that a T-DNA insertion into the VTC2 causes a similar reduction in ascorbate levels, but does not greatly affect plant growth. Subsequent segregation analysis revealed the growth defects of vtc2-1 mutants segregate independently of the vtc2-1 mutation. These observations suggest the presence of an independent cryptic mutation that affects growth in vtc2-1 mutants and that a 70-80% decrease in ascorbate is not sufficient to a dramatically alter plant growth as has been assumed in past studies.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01025/fullArabidopsisvitamin Cascorbatevtc2vtc5GDP-L-galactose phosphorylase |
| spellingShingle | Benson Lim Nicholas Smirnoff Christopher S Cobbett John F Golz Ascorbate-deficient vtc2 mutants in Arabidopsis do not exhibit decreased growth Frontiers in Plant Science Arabidopsis vitamin C ascorbate vtc2 vtc5 GDP-L-galactose phosphorylase |
| title | Ascorbate-deficient vtc2 mutants in Arabidopsis do not exhibit decreased growth |
| title_full | Ascorbate-deficient vtc2 mutants in Arabidopsis do not exhibit decreased growth |
| title_fullStr | Ascorbate-deficient vtc2 mutants in Arabidopsis do not exhibit decreased growth |
| title_full_unstemmed | Ascorbate-deficient vtc2 mutants in Arabidopsis do not exhibit decreased growth |
| title_short | Ascorbate-deficient vtc2 mutants in Arabidopsis do not exhibit decreased growth |
| title_sort | ascorbate deficient vtc2 mutants in arabidopsis do not exhibit decreased growth |
| topic | Arabidopsis vitamin C ascorbate vtc2 vtc5 GDP-L-galactose phosphorylase |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01025/full |
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