An approach to quantitate maternal transcripts localized in sea urchin egg cortex using RT-qPCR with accurate normalization.
The sea urchin egg cortex is a peripheral region of eggs comprising a cell membrane and adjacent cytoplasm, which contains actin and tubulin cytoskeleton, cortical granules and some proteins required for early development. Method for isolation of cortices from sea urchin eggs and early embryos was d...
| Հիմնական հեղինակներ: | , , |
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| Ձևաչափ: | Հոդված |
| Լեզու: | English |
| Հրապարակվել է: |
Public Library of Science (PLoS)
2022-01-01
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| Շարք: | PLoS ONE |
| Առցանց հասանելիություն: | https://doi.org/10.1371/journal.pone.0260831 |