Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC
An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous determination of gemifloxacin and diuretics (hydrochlorothiazide and furosemide) in bulk, dosage formulations and human serum at 232 nm. Chromatographic separation was achieve...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2010-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700029 |
| _version_ | 1818700322323824640 |
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| author | Najma Sultana Sana Shamim Mahwish Akhtarand Somia Gul M. Saeed Arayne |
| author_facet | Najma Sultana Sana Shamim Mahwish Akhtarand Somia Gul M. Saeed Arayne |
| author_sort | Najma Sultana |
| collection | DOAJ |
| description | An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous determination of gemifloxacin and diuretics (hydrochlorothiazide and furosemide) in bulk, dosage formulations and human serum at 232 nm. Chromatographic separation was achieved on Purospher Start C18 (250 mm x 4.6 mm, 5 µm) column using mobile phase, methanol: water: acetonitrile (70:25:5 v/v/v) adjusted to pH 3.0 via phosphoric acid 85% having flow rate of 0.8 mL min -1 at room temperature. Calibration curves were linear over range of 0.5-10 µg mL -1 with a correlation coefficient ± 0.999. LOD and LOQ were in the ranges of 0.75-2.56 µg mL -1. Intra and inter-run precision and accuracy results were 98.26 to 100.9. |
| first_indexed | 2024-12-17T15:03:06Z |
| format | Article |
| id | doaj.art-d5ce3711076d424a8a39bc89bcc3a32c |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-12-17T15:03:06Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-d5ce3711076d424a8a39bc89bcc3a32c2022-12-21T21:43:51ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642010-01-013371590159310.1590/S0100-40422010000700029Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLCNajma SultanaSana ShamimMahwish Akhtarand Somia GulM. Saeed ArayneAn isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous determination of gemifloxacin and diuretics (hydrochlorothiazide and furosemide) in bulk, dosage formulations and human serum at 232 nm. Chromatographic separation was achieved on Purospher Start C18 (250 mm x 4.6 mm, 5 µm) column using mobile phase, methanol: water: acetonitrile (70:25:5 v/v/v) adjusted to pH 3.0 via phosphoric acid 85% having flow rate of 0.8 mL min -1 at room temperature. Calibration curves were linear over range of 0.5-10 µg mL -1 with a correlation coefficient ± 0.999. LOD and LOQ were in the ranges of 0.75-2.56 µg mL -1. Intra and inter-run precision and accuracy results were 98.26 to 100.9.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700029gemifloxacindiureticsHPLC |
| spellingShingle | Najma Sultana Sana Shamim Mahwish Akhtarand Somia Gul M. Saeed Arayne Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC Química Nova gemifloxacin diuretics HPLC |
| title | Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC |
| title_full | Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC |
| title_fullStr | Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC |
| title_full_unstemmed | Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC |
| title_short | Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC |
| title_sort | simultaneous determination of gemifloxacin and diuretics in bulk pharmaceutical dosage forms and human serum by rp hplc |
| topic | gemifloxacin diuretics HPLC |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700029 |
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