Histological Evaluation of Cassava Starch/Chicken Gelatin Membranes
The use of biopolymers for tissue engineering has recently gained attention due to the need for safer and highly compatible materials. Starch is one of the most used biopolymers for membrane preparation. However, incorporating other polymers into starch membranes introduces improvements, such as bet...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/18/3849 |
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| author | Carlos Humberto Valencia-Llano Jorge Iván Castro Marcela Saavedra Paula A. Zapata Diana Paola Navia-Porras Edwin Flórez-López Carolina Caicedo Heidy Lorena Calambas Carlos David Grande-Tovar |
| author_facet | Carlos Humberto Valencia-Llano Jorge Iván Castro Marcela Saavedra Paula A. Zapata Diana Paola Navia-Porras Edwin Flórez-López Carolina Caicedo Heidy Lorena Calambas Carlos David Grande-Tovar |
| author_sort | Carlos Humberto Valencia-Llano |
| collection | DOAJ |
| description | The use of biopolymers for tissue engineering has recently gained attention due to the need for safer and highly compatible materials. Starch is one of the most used biopolymers for membrane preparation. However, incorporating other polymers into starch membranes introduces improvements, such as better thermal and mechanical resistance and increased water affinity, as we reported in our previous work. There are few reports in the literature on the biocompatibility of starch/chicken gelatin composites. We assessed the in vivo biocompatibility of the five composites (T1–T5) cassava starch/gelatin membranes with subdermal implantations in biomodels at 30, 60, and 90 days. The FT-IR spectroscopy analysis demonstrated the main functional groups for starch and chicken gelatin. At the same time, the thermal study exhibited an increase in thermal resistance for T3 and T4, with a remaining mass (~15 wt.%) at 800 °C. The microstructure analysis for the T2–T4 demonstrated evident roughness changes with porosity presence due to starch and gelatin mixture. The decrease in the starch content in the composites also decreased the gelatinization heats for T3 and T4 (195.67, 196.40 J/g, respectively). Finally, the implantation results demonstrated that the formulations exhibited differences in the degradation and resorption capacities according to the starch content, which is easily degraded by amylases. However, the histological results showed that the samples demonstrated almost complete reabsorption without a severe immune response, indicating a high in vivo biocompatibility. These results show that the cassava starch/chicken gelatin composites are promising membrane materials for tissue engineering applications. |
| first_indexed | 2024-03-09T22:45:54Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T22:45:54Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-d5cf9854fe1b495fb380efc07ef0d99c2023-11-23T18:30:56ZengMDPI AGPolymers2073-43602022-09-011418384910.3390/polym14183849Histological Evaluation of Cassava Starch/Chicken Gelatin MembranesCarlos Humberto Valencia-Llano0Jorge Iván Castro1Marcela Saavedra2Paula A. Zapata3Diana Paola Navia-Porras4Edwin Flórez-López5Carolina Caicedo6Heidy Lorena Calambas7Carlos David Grande-Tovar8Research Group in Biomateriales Dentales, School of Odontología, Faculty of Health, Campus San Fernando, Universidad del Valle, Calle 4B # 36-00, Cali 76001, ColombiaResearch Group SIMERQO, Department of Chemistry, Faculty of Natural and Exact Sciences, Campus Melendez, Universidad del Valle, Calle 13 No. 100-00, Santiago de Cali 76001, ColombiaResearch Group of Polímeros, Department of Chemistry, Faculty de Chemistry and Biology, Universidad de Santiago de Chile, USACH, Santiago 9170020, ChileResearch Group of Polímeros, Department of Chemistry, Faculty de Chemistry and Biology, Universidad de Santiago de Chile, USACH, Santiago 9170020, ChileResearch Group Biotecnología, Faculty of Engineering, Universidad de San Buenaventura Cali, Carrera 122 # 6-65, Santiago de Cali 76001, ColombiaResearch Group in Química y Biotecnología QUIBIO, Universidad Santiago de Cali, Calle 5 No 62-00, Cali 760035, ColombiaResearch Group GIGAE3D, Faculty of Engineering, Unidad Central del Valle del Cauca (UCEVA), Carrera 17ª 48-144, Tuluá 763022, ColombiaResearch Group in Desarrollo de Materiales y Productos, Centro Nacional de Asistencia Técnica a la Industria (ASTIN), SENA, Cali 760003, ColombiaResearch Group of Fotoquímica y Fotobiología, Universidad del Atlántico, Carrera 30 Número 8-49, Puerto Colombia 081008, ColombiaThe use of biopolymers for tissue engineering has recently gained attention due to the need for safer and highly compatible materials. Starch is one of the most used biopolymers for membrane preparation. However, incorporating other polymers into starch membranes introduces improvements, such as better thermal and mechanical resistance and increased water affinity, as we reported in our previous work. There are few reports in the literature on the biocompatibility of starch/chicken gelatin composites. We assessed the in vivo biocompatibility of the five composites (T1–T5) cassava starch/gelatin membranes with subdermal implantations in biomodels at 30, 60, and 90 days. The FT-IR spectroscopy analysis demonstrated the main functional groups for starch and chicken gelatin. At the same time, the thermal study exhibited an increase in thermal resistance for T3 and T4, with a remaining mass (~15 wt.%) at 800 °C. The microstructure analysis for the T2–T4 demonstrated evident roughness changes with porosity presence due to starch and gelatin mixture. The decrease in the starch content in the composites also decreased the gelatinization heats for T3 and T4 (195.67, 196.40 J/g, respectively). Finally, the implantation results demonstrated that the formulations exhibited differences in the degradation and resorption capacities according to the starch content, which is easily degraded by amylases. However, the histological results showed that the samples demonstrated almost complete reabsorption without a severe immune response, indicating a high in vivo biocompatibility. These results show that the cassava starch/chicken gelatin composites are promising membrane materials for tissue engineering applications.https://www.mdpi.com/2073-4360/14/18/3849biocompatibilitybiocompositecassava starchchicken gelatincomposite membranestissue engineering |
| spellingShingle | Carlos Humberto Valencia-Llano Jorge Iván Castro Marcela Saavedra Paula A. Zapata Diana Paola Navia-Porras Edwin Flórez-López Carolina Caicedo Heidy Lorena Calambas Carlos David Grande-Tovar Histological Evaluation of Cassava Starch/Chicken Gelatin Membranes Polymers biocompatibility biocomposite cassava starch chicken gelatin composite membranes tissue engineering |
| title | Histological Evaluation of Cassava Starch/Chicken Gelatin Membranes |
| title_full | Histological Evaluation of Cassava Starch/Chicken Gelatin Membranes |
| title_fullStr | Histological Evaluation of Cassava Starch/Chicken Gelatin Membranes |
| title_full_unstemmed | Histological Evaluation of Cassava Starch/Chicken Gelatin Membranes |
| title_short | Histological Evaluation of Cassava Starch/Chicken Gelatin Membranes |
| title_sort | histological evaluation of cassava starch chicken gelatin membranes |
| topic | biocompatibility biocomposite cassava starch chicken gelatin composite membranes tissue engineering |
| url | https://www.mdpi.com/2073-4360/14/18/3849 |
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