Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence.
Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current comm...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4743966?pdf=render |
| _version_ | 1828425613322485760 |
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| author | Zachary J Farino Travis J Morgenstern Julie Vallaghe Nathalie Gregor Prashant Donthamsetti Paul E Harris Nicolas Pierre Robin Freyberg Fabienne Charrier-Savournin Jonathan A Javitch Zachary Freyberg |
| author_facet | Zachary J Farino Travis J Morgenstern Julie Vallaghe Nathalie Gregor Prashant Donthamsetti Paul E Harris Nicolas Pierre Robin Freyberg Fabienne Charrier-Savournin Jonathan A Javitch Zachary Freyberg |
| author_sort | Zachary J Farino |
| collection | DOAJ |
| description | Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current commonly used approaches. This assay was applied effectively to an insulin secreting cell line, INS-1E cells, as well as pancreatic islets, allowing us to validate the assay by elucidating mechanisms by which dopamine regulates insulin release. We found that dopamine functioned as a significant negative modulator of glucose-stimulated insulin secretion. Further, we showed that bromocriptine, a known dopamine D2/D3 receptor agonist and newly approved drug used for treatment of type II diabetes mellitus, also decreased glucose-stimulated insulin secretion in islets to levels comparable to those caused by dopamine treatment. |
| first_indexed | 2024-12-10T16:33:20Z |
| format | Article |
| id | doaj.art-d5d1b9e483904f1da5f84e0c75cfd217 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T16:33:20Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d5d1b9e483904f1da5f84e0c75cfd2172022-12-22T01:41:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01112e014868410.1371/journal.pone.0148684Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence.Zachary J FarinoTravis J MorgensternJulie VallagheNathalie GregorPrashant DonthamsettiPaul E HarrisNicolas PierreRobin FreybergFabienne Charrier-SavourninJonathan A JavitchZachary FreybergDirect measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current commonly used approaches. This assay was applied effectively to an insulin secreting cell line, INS-1E cells, as well as pancreatic islets, allowing us to validate the assay by elucidating mechanisms by which dopamine regulates insulin release. We found that dopamine functioned as a significant negative modulator of glucose-stimulated insulin secretion. Further, we showed that bromocriptine, a known dopamine D2/D3 receptor agonist and newly approved drug used for treatment of type II diabetes mellitus, also decreased glucose-stimulated insulin secretion in islets to levels comparable to those caused by dopamine treatment.http://europepmc.org/articles/PMC4743966?pdf=render |
| spellingShingle | Zachary J Farino Travis J Morgenstern Julie Vallaghe Nathalie Gregor Prashant Donthamsetti Paul E Harris Nicolas Pierre Robin Freyberg Fabienne Charrier-Savournin Jonathan A Javitch Zachary Freyberg Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence. PLoS ONE |
| title | Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence. |
| title_full | Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence. |
| title_fullStr | Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence. |
| title_full_unstemmed | Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence. |
| title_short | Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence. |
| title_sort | development of a rapid insulin assay by homogenous time resolved fluorescence |
| url | http://europepmc.org/articles/PMC4743966?pdf=render |
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