Evolution of Two Receptors detecting the Same Pheromone Compound in Crop Pest Moths of the Genus Spodoptera
In moths, mate finding strongly rely on the detection of sex pheromones by pheromone receptors. Any modification in the functional properties of these receptors can have a drastic impact on reproduction. In the course of characterizing candidate pheromone receptors in the noctuid moth Spodoptera lit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-08-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fevo.2015.00095/full |
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| author | Arthur ede Fouchier Arthur ede Fouchier Xiao eSun Xiao eSun Xiao eSun Christelle eMonsempes Christelle eMonsempes Olivier eMirabeau Emmanuelle eJacquin-Joly Emmanuelle eJacquin-Joly Nicolas eMontagné Nicolas eMontagné |
| author_facet | Arthur ede Fouchier Arthur ede Fouchier Xiao eSun Xiao eSun Xiao eSun Christelle eMonsempes Christelle eMonsempes Olivier eMirabeau Emmanuelle eJacquin-Joly Emmanuelle eJacquin-Joly Nicolas eMontagné Nicolas eMontagné |
| author_sort | Arthur ede Fouchier |
| collection | DOAJ |
| description | In moths, mate finding strongly rely on the detection of sex pheromones by pheromone receptors. Any modification in the functional properties of these receptors can have a drastic impact on reproduction. In the course of characterizing candidate pheromone receptors in the noctuid moth Spodoptera littoralis, we expressed them in Drosophila olfactory sensory neurons and stimulated them with a large panel of moth pheromone compounds. We found that two pheromone receptors detect (Z,E)-9,12-14:OAc, a minor component of the female pheromone blend. Whereas SlitOR6 is highly specific to this component, SlitOR13 is less sensitive and not strictly specific as it also detects (Z)9-14:OAc, another minor component of the sex pheromone. Interestingly, SlitOR13 expression is restricted to the distal part of male antennae, where we could identify a novel functional class of pheromone-sensitive neurons whose response spectrum matches that of SlitOR13. Based on a phylogenetic analysis of Lepidoptera pheromone receptors, we built an evolutionary scenario in which four different paralogous lineages emerged through gene duplications. The ability to bind (Z,E)-9,12-14:OAc appeared independently within three of these lineages, and an analysis of selective pressures revealed sites under positive selection that could have played a role in the emergence of functional properties of OR6 and OR13 in Spodoptera species. |
| first_indexed | 2024-12-11T01:57:49Z |
| format | Article |
| id | doaj.art-d5d2625a8111430d931a5ab99c89e7e8 |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-12-11T01:57:49Z |
| publishDate | 2015-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-d5d2625a8111430d931a5ab99c89e7e82022-12-22T01:24:34ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2015-08-01310.3389/fevo.2015.00095152571Evolution of Two Receptors detecting the Same Pheromone Compound in Crop Pest Moths of the Genus SpodopteraArthur ede Fouchier0Arthur ede Fouchier1Xiao eSun2Xiao eSun3Xiao eSun4Christelle eMonsempes5Christelle eMonsempes6Olivier eMirabeau7Emmanuelle eJacquin-Joly8Emmanuelle eJacquin-Joly9Nicolas eMontagné10Nicolas eMontagné11INRAUniversité Pierre et Marie CurieINRAUniversité Pierre et Marie CurieHuazhong Agricultural UniversityINRAUniversité Pierre et Marie CurieInstitut CurieINRAUniversité Pierre et Marie CurieUniversité Pierre et Marie CurieINRAIn moths, mate finding strongly rely on the detection of sex pheromones by pheromone receptors. Any modification in the functional properties of these receptors can have a drastic impact on reproduction. In the course of characterizing candidate pheromone receptors in the noctuid moth Spodoptera littoralis, we expressed them in Drosophila olfactory sensory neurons and stimulated them with a large panel of moth pheromone compounds. We found that two pheromone receptors detect (Z,E)-9,12-14:OAc, a minor component of the female pheromone blend. Whereas SlitOR6 is highly specific to this component, SlitOR13 is less sensitive and not strictly specific as it also detects (Z)9-14:OAc, another minor component of the sex pheromone. Interestingly, SlitOR13 expression is restricted to the distal part of male antennae, where we could identify a novel functional class of pheromone-sensitive neurons whose response spectrum matches that of SlitOR13. Based on a phylogenetic analysis of Lepidoptera pheromone receptors, we built an evolutionary scenario in which four different paralogous lineages emerged through gene duplications. The ability to bind (Z,E)-9,12-14:OAc appeared independently within three of these lineages, and an analysis of selective pressures revealed sites under positive selection that could have played a role in the emergence of functional properties of OR6 and OR13 in Spodoptera species.http://journal.frontiersin.org/Journal/10.3389/fevo.2015.00095/fullinsectOlfactionheterologous expressionpositive selectionolfactory receptorsex pheromone |
| spellingShingle | Arthur ede Fouchier Arthur ede Fouchier Xiao eSun Xiao eSun Xiao eSun Christelle eMonsempes Christelle eMonsempes Olivier eMirabeau Emmanuelle eJacquin-Joly Emmanuelle eJacquin-Joly Nicolas eMontagné Nicolas eMontagné Evolution of Two Receptors detecting the Same Pheromone Compound in Crop Pest Moths of the Genus Spodoptera Frontiers in Ecology and Evolution insect Olfaction heterologous expression positive selection olfactory receptor sex pheromone |
| title | Evolution of Two Receptors detecting the Same Pheromone Compound in Crop Pest Moths of the Genus Spodoptera |
| title_full | Evolution of Two Receptors detecting the Same Pheromone Compound in Crop Pest Moths of the Genus Spodoptera |
| title_fullStr | Evolution of Two Receptors detecting the Same Pheromone Compound in Crop Pest Moths of the Genus Spodoptera |
| title_full_unstemmed | Evolution of Two Receptors detecting the Same Pheromone Compound in Crop Pest Moths of the Genus Spodoptera |
| title_short | Evolution of Two Receptors detecting the Same Pheromone Compound in Crop Pest Moths of the Genus Spodoptera |
| title_sort | evolution of two receptors detecting the same pheromone compound in crop pest moths of the genus spodoptera |
| topic | insect Olfaction heterologous expression positive selection olfactory receptor sex pheromone |
| url | http://journal.frontiersin.org/Journal/10.3389/fevo.2015.00095/full |
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