A comparison of methods to determine tannin acyl hydrolase activity
Six methods to determine the activity of tannase produced by Aspergillus niger Aa-20 on polyurethane foam by solid state fermentation, which included two titrimetric techniques, three spectrophotometric methods and one HPLC assay were tested and compared. All methods assayed enabled the measurement...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
1999-01-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300014 |
| _version_ | 1819042916093394944 |
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| author | Cristóbal Aguilar Augur Christopher Gustavo Viniegra-González Ernesto Favela |
| author_facet | Cristóbal Aguilar Augur Christopher Gustavo Viniegra-González Ernesto Favela |
| author_sort | Cristóbal Aguilar |
| collection | DOAJ |
| description | Six methods to determine the activity of tannase produced by Aspergillus niger Aa-20 on polyurethane foam by solid state fermentation, which included two titrimetric techniques, three spectrophotometric methods and one HPLC assay were tested and compared. All methods assayed enabled the measurement of extracellular tannase activity. However, only five were useful to evaluate intracellular tannase activity. Studies on the effect of pH on tannase extraction demonstrated that tannase activity was considerably under-estimated when its extraction was carried out at pH values below 5.5 and above 6.0. Results showed that the HPLC technique and the modified Bajpai and Patil methods presented several advantages in comparison to the other methods tested.<br>Seis métodos para determinar a atividade de tannase produzida por Aspergillus niger O Aa-20 em espuma de polyuretano por fermentação em estado sólido foram estudados. Duas técnicas titulométricas , três métodos spectrofotométricos e um método por HPLC foram testados e comparados. Todos os métodos testados permitiram determinar a atividade da tannase produzida extracelularmente. Entretanto, somente cinco se mostraram úteis para avaliar a atividade da tannase produzida intracelularmente. Os estudos do efeito do pH na extração de tannase demonstraram que a atividade de tannase era consideravelmente subestimada quando sua extração foi executada em valores de pH inferiores a 5.5 e superior a pH 6.0. Os resultados demostraram que a técnica de HPLC o método Bajpai and Patil modificado apresentam várias vantagens em comparação aos outros métodos testados. |
| first_indexed | 2024-12-21T09:48:29Z |
| format | Article |
| id | doaj.art-d5d4223054f945c9a4ac7536ea86164b |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-21T09:48:29Z |
| publishDate | 1999-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-d5d4223054f945c9a4ac7536ea86164b2022-12-21T19:08:16ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43241999-01-0142335536210.1590/S1516-89131999000300014A comparison of methods to determine tannin acyl hydrolase activityCristóbal AguilarAugur ChristopherGustavo Viniegra-GonzálezErnesto FavelaSix methods to determine the activity of tannase produced by Aspergillus niger Aa-20 on polyurethane foam by solid state fermentation, which included two titrimetric techniques, three spectrophotometric methods and one HPLC assay were tested and compared. All methods assayed enabled the measurement of extracellular tannase activity. However, only five were useful to evaluate intracellular tannase activity. Studies on the effect of pH on tannase extraction demonstrated that tannase activity was considerably under-estimated when its extraction was carried out at pH values below 5.5 and above 6.0. Results showed that the HPLC technique and the modified Bajpai and Patil methods presented several advantages in comparison to the other methods tested.<br>Seis métodos para determinar a atividade de tannase produzida por Aspergillus niger O Aa-20 em espuma de polyuretano por fermentação em estado sólido foram estudados. Duas técnicas titulométricas , três métodos spectrofotométricos e um método por HPLC foram testados e comparados. Todos os métodos testados permitiram determinar a atividade da tannase produzida extracelularmente. Entretanto, somente cinco se mostraram úteis para avaliar a atividade da tannase produzida intracelularmente. Os estudos do efeito do pH na extração de tannase demonstraram que a atividade de tannase era consideravelmente subestimada quando sua extração foi executada em valores de pH inferiores a 5.5 e superior a pH 6.0. Os resultados demostraram que a técnica de HPLC o método Bajpai and Patil modificado apresentam várias vantagens em comparação aos outros métodos testados.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300014Tannin acyl hydrolaseassaypolyurethane foamsolid state fermentationtannase |
| spellingShingle | Cristóbal Aguilar Augur Christopher Gustavo Viniegra-González Ernesto Favela A comparison of methods to determine tannin acyl hydrolase activity Brazilian Archives of Biology and Technology Tannin acyl hydrolase assay polyurethane foam solid state fermentation tannase |
| title | A comparison of methods to determine tannin acyl hydrolase activity |
| title_full | A comparison of methods to determine tannin acyl hydrolase activity |
| title_fullStr | A comparison of methods to determine tannin acyl hydrolase activity |
| title_full_unstemmed | A comparison of methods to determine tannin acyl hydrolase activity |
| title_short | A comparison of methods to determine tannin acyl hydrolase activity |
| title_sort | comparison of methods to determine tannin acyl hydrolase activity |
| topic | Tannin acyl hydrolase assay polyurethane foam solid state fermentation tannase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300014 |
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