Inflammasomes in rheumatoid arthritis: a pilot study
Abstract Background The inflammasome plays an important role in rheumatoid arthritis (RA), which has rarely been systematically reported. The aim of this study was to understand whether the levels of inflammasomes were related to the severity of RA disease, which might provide a stronger theoretical...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Rheumatology |
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| Online Access: | https://doi.org/10.1186/s41927-023-00353-8 |
| _version_ | 1827770330451542016 |
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| author | Qi Jiang Xin Wang Xiuping Xu Liangfeng Hu Guozhong Zhou Rui Liu Guocan Yang Dawei Cui |
| author_facet | Qi Jiang Xin Wang Xiuping Xu Liangfeng Hu Guozhong Zhou Rui Liu Guocan Yang Dawei Cui |
| author_sort | Qi Jiang |
| collection | DOAJ |
| description | Abstract Background The inflammasome plays an important role in rheumatoid arthritis (RA), which has rarely been systematically reported. The aim of this study was to understand whether the levels of inflammasomes were related to the severity of RA disease, which might provide a stronger theoretical basis for RA treatment. Methods The mRNA expression levels of some inflammasomes and associated molecules, including IL-1beta and IL-18, in peripheral blood mononuclear cells (PBMCs) from 30 RA patients (n = 30) and 16 healthy control (HC) individuals were determined by quantitative real-time polymerase chain reaction (qRT‒PCR), and the levels of plasma IL-1beta and IL-18 were also measured by enzyme-linked immunosorbent assay (ELISA). Moreover, the clinical characteristics and laboratory results of the patients were collected and analyzed in this study. Results The relative mRNA expression levels of NLRP3, NLRC4, AIM2, caspase-1, and IL-1beta were significantly higher and those of NLRP1, NLRP2 and NLRC5 were notably lower in the HC group than in the RA group. Moreover, the plasma IL-1beta and IL-18 levels were markedly increased in the RA group. Additionally, the mRNA level of AIM2 was negatively correlated with disease activity score 28 (DAS28) by stepwise linear regression analysis. erythrocyte sedimentation rate (ESR) was positively correlated with DAS28 by multiple linear regression analysis in the RA group. Conclusions These findings imply the critical role of NLRP3, NLRC4, AIM2, caspase-1 and plasma IL-1beta and IL-18 in the pathogenesis of RA patients, which provides potential targets for the treatment of RA. |
| first_indexed | 2024-03-11T12:38:02Z |
| format | Article |
| id | doaj.art-d5d65921816148bcad69f017b36a62b5 |
| institution | Directory Open Access Journal |
| issn | 2520-1026 |
| language | English |
| last_indexed | 2024-03-11T12:38:02Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Rheumatology |
| spelling | doaj.art-d5d65921816148bcad69f017b36a62b52023-11-05T12:32:03ZengBMCBMC Rheumatology2520-10262023-10-017111010.1186/s41927-023-00353-8Inflammasomes in rheumatoid arthritis: a pilot studyQi Jiang0Xin Wang1Xiuping Xu2Liangfeng Hu3Guozhong Zhou4Rui Liu5Guocan Yang6Dawei Cui7Department of Blood Transfusion, Shaoxing People’s HospitalDepartment of Rheumatology and Immunology, Shaoxing People’s HospitalDepartment of Clinical Laboratory Center, Shaoxing People’s HospitalDepartment of Clinical Laboratory Center, Shaoxing People’s HospitalDepartment of Clinical Laboratory Center, Shaoxing People’s HospitalDepartment of Rheumatology, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese MedicineDepartment of Blood Transfusion, Shaoxing People’s HospitalDepartment of Blood Transfusion, the First Affiliated Hospital, Zhejiang University School of MedicineAbstract Background The inflammasome plays an important role in rheumatoid arthritis (RA), which has rarely been systematically reported. The aim of this study was to understand whether the levels of inflammasomes were related to the severity of RA disease, which might provide a stronger theoretical basis for RA treatment. Methods The mRNA expression levels of some inflammasomes and associated molecules, including IL-1beta and IL-18, in peripheral blood mononuclear cells (PBMCs) from 30 RA patients (n = 30) and 16 healthy control (HC) individuals were determined by quantitative real-time polymerase chain reaction (qRT‒PCR), and the levels of plasma IL-1beta and IL-18 were also measured by enzyme-linked immunosorbent assay (ELISA). Moreover, the clinical characteristics and laboratory results of the patients were collected and analyzed in this study. Results The relative mRNA expression levels of NLRP3, NLRC4, AIM2, caspase-1, and IL-1beta were significantly higher and those of NLRP1, NLRP2 and NLRC5 were notably lower in the HC group than in the RA group. Moreover, the plasma IL-1beta and IL-18 levels were markedly increased in the RA group. Additionally, the mRNA level of AIM2 was negatively correlated with disease activity score 28 (DAS28) by stepwise linear regression analysis. erythrocyte sedimentation rate (ESR) was positively correlated with DAS28 by multiple linear regression analysis in the RA group. Conclusions These findings imply the critical role of NLRP3, NLRC4, AIM2, caspase-1 and plasma IL-1beta and IL-18 in the pathogenesis of RA patients, which provides potential targets for the treatment of RA.https://doi.org/10.1186/s41927-023-00353-8InflammasomeRheumatoid arthritisNLRP3NLRC4AIM2 |
| spellingShingle | Qi Jiang Xin Wang Xiuping Xu Liangfeng Hu Guozhong Zhou Rui Liu Guocan Yang Dawei Cui Inflammasomes in rheumatoid arthritis: a pilot study BMC Rheumatology Inflammasome Rheumatoid arthritis NLRP3 NLRC4 AIM2 |
| title | Inflammasomes in rheumatoid arthritis: a pilot study |
| title_full | Inflammasomes in rheumatoid arthritis: a pilot study |
| title_fullStr | Inflammasomes in rheumatoid arthritis: a pilot study |
| title_full_unstemmed | Inflammasomes in rheumatoid arthritis: a pilot study |
| title_short | Inflammasomes in rheumatoid arthritis: a pilot study |
| title_sort | inflammasomes in rheumatoid arthritis a pilot study |
| topic | Inflammasome Rheumatoid arthritis NLRP3 NLRC4 AIM2 |
| url | https://doi.org/10.1186/s41927-023-00353-8 |
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