Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body.
Higher fungi, which comprise ascomycetes and basidiomycetes, play major roles in the biosphere. Their evolutionary success may be due to the extended dikaryotic stage of their life cycle, which is the basis for their scientific name: the Dikarya. Dikaryosis is maintained by similar structures, the c...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3360767?pdf=render |
| _version_ | 1831749302350774272 |
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| author | Evelyne Coppin Véronique Berteaux-Lecellier Frédérique Bidard Sylvain Brun Gwenaël Ruprich-Robert Eric Espagne Jinane Aït-Benkhali Anne Goarin Audrey Nesseir Sara Planamente Robert Debuchy Philippe Silar |
| author_facet | Evelyne Coppin Véronique Berteaux-Lecellier Frédérique Bidard Sylvain Brun Gwenaël Ruprich-Robert Eric Espagne Jinane Aït-Benkhali Anne Goarin Audrey Nesseir Sara Planamente Robert Debuchy Philippe Silar |
| author_sort | Evelyne Coppin |
| collection | DOAJ |
| description | Higher fungi, which comprise ascomycetes and basidiomycetes, play major roles in the biosphere. Their evolutionary success may be due to the extended dikaryotic stage of their life cycle, which is the basis for their scientific name: the Dikarya. Dikaryosis is maintained by similar structures, the clamp in basidiomycetes and the crozier in ascomycetes. Homeodomain transcription factors are required for clamp formation in all basidiomycetes studied. We identified all the homeobox genes in the filamentous ascomycete fungus Podospora anserina and constructed deletion mutants for each of these genes and for a number of gene combinations. Croziers developed normally in these mutants, including those with up to six deleted homeogenes. However, some mutants had defects in maturation of the fruiting body, an effect that could be rescued by providing wild-type maternal hyphae. Analysis of mutants deficient in multiple homeogenes revealed interactions between the genes, suggesting that they operate as a complex network. Similar to their role in animals and plants, homeodomain transcription factors in ascomycetes are involved in shaping multicellular structures. |
| first_indexed | 2024-12-21T22:08:42Z |
| format | Article |
| id | doaj.art-d5d846c4d26c447e95e137ede77e072d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T22:08:42Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d5d846c4d26c447e95e137ede77e072d2022-12-21T18:48:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3748810.1371/journal.pone.0037488Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body.Evelyne CoppinVéronique Berteaux-LecellierFrédérique BidardSylvain BrunGwenaël Ruprich-RobertEric EspagneJinane Aït-BenkhaliAnne GoarinAudrey NesseirSara PlanamenteRobert DebuchyPhilippe SilarHigher fungi, which comprise ascomycetes and basidiomycetes, play major roles in the biosphere. Their evolutionary success may be due to the extended dikaryotic stage of their life cycle, which is the basis for their scientific name: the Dikarya. Dikaryosis is maintained by similar structures, the clamp in basidiomycetes and the crozier in ascomycetes. Homeodomain transcription factors are required for clamp formation in all basidiomycetes studied. We identified all the homeobox genes in the filamentous ascomycete fungus Podospora anserina and constructed deletion mutants for each of these genes and for a number of gene combinations. Croziers developed normally in these mutants, including those with up to six deleted homeogenes. However, some mutants had defects in maturation of the fruiting body, an effect that could be rescued by providing wild-type maternal hyphae. Analysis of mutants deficient in multiple homeogenes revealed interactions between the genes, suggesting that they operate as a complex network. Similar to their role in animals and plants, homeodomain transcription factors in ascomycetes are involved in shaping multicellular structures.http://europepmc.org/articles/PMC3360767?pdf=render |
| spellingShingle | Evelyne Coppin Véronique Berteaux-Lecellier Frédérique Bidard Sylvain Brun Gwenaël Ruprich-Robert Eric Espagne Jinane Aït-Benkhali Anne Goarin Audrey Nesseir Sara Planamente Robert Debuchy Philippe Silar Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body. PLoS ONE |
| title | Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body. |
| title_full | Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body. |
| title_fullStr | Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body. |
| title_full_unstemmed | Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body. |
| title_short | Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body. |
| title_sort | systematic deletion of homeobox genes in podospora anserina uncovers their roles in shaping the fruiting body |
| url | http://europepmc.org/articles/PMC3360767?pdf=render |
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