Spawning Performance and Sex Steroid Levels in Female Pikeperch <i>Sander lucioperca</i> Treated with Poly(lactic-co-glycolic acid) Microparticles
Pikeperch <i>Sander lucioperca</i> is a piscivorous species considered a promising candidate for the diversification of intensive aquaculture. This study aimed to determine the effect of a sustained-release delivery system incorporating mammalian gonadotropin-releasing hormone agonist (m...
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MDPI AG
2022-01-01
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| author | Jindřiška Knowles Jakub Vysloužil Tomáš Policar Sylvain Milla Martina Holická Peter Podhorec |
| author_facet | Jindřiška Knowles Jakub Vysloužil Tomáš Policar Sylvain Milla Martina Holická Peter Podhorec |
| author_sort | Jindřiška Knowles |
| collection | DOAJ |
| description | Pikeperch <i>Sander lucioperca</i> is a piscivorous species considered a promising candidate for the diversification of intensive aquaculture. This study aimed to determine the effect of a sustained-release delivery system incorporating mammalian gonadotropin-releasing hormone agonist (mGnRHa) into poly(lactic-co-glycolic acid) (PLGA) microparticles on the sex steroid levels and aspects of artificial reproduction of pikeperch. Fish were divided into four groups and injected with 20 µg mGnRHa/kg, 5-day release microparticles encapsulated with 5 µg GnRHa/kg BW (PLGA 5), 20 µg GnRHa/kg (PLGA 20), or 1 mL/kg 0.9% NaCl (control). Cumulative percentage ovulation was 100% in the PLGA 5 group, significantly higher than in other tested groups. No differences among groups were observed in latency or fecundity. The level of 11-ketotestosterone (11-KT) peaked at 40 h post-injection, and was sustained during ovulation, in all treated groups. The 17β-estradiol (E2) concentration increased in the mGnRHa-only group immediately after hormone injection, while both PLGA groups showed a reduction in E2 after injection, continuing to decrease until ovulation. A low dose of mGnRHa in PLGA microparticles significantly improves induction of ovulation and results in acceptable reproductive performance, which may positively affect pikeperch production under controlled conditions. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T03:03:08Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-d5dc4fe8b1e54137a3aa1b584d7981772023-11-23T12:42:16ZengMDPI AGAnimals2076-26152022-01-0112220810.3390/ani12020208Spawning Performance and Sex Steroid Levels in Female Pikeperch <i>Sander lucioperca</i> Treated with Poly(lactic-co-glycolic acid) MicroparticlesJindřiška Knowles0Jakub Vysloužil1Tomáš Policar2Sylvain Milla3Martina Holická4Peter Podhorec5Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in České Budějovice, Zátiší 728/II, 38925 Vodňany, Czech RepublicDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, Palackého třída 1946/1, 61200 Brno, Czech RepublicFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in České Budějovice, Zátiší 728/II, 38925 Vodňany, Czech RepublicUnit of Animal Research and Functionalities of Animal Products, University of Lorraine, INRA 340, 54506 Vandoeuvre-l`es-Nancy, FranceDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, Palackého třída 1946/1, 61200 Brno, Czech RepublicFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in České Budějovice, Zátiší 728/II, 38925 Vodňany, Czech RepublicPikeperch <i>Sander lucioperca</i> is a piscivorous species considered a promising candidate for the diversification of intensive aquaculture. This study aimed to determine the effect of a sustained-release delivery system incorporating mammalian gonadotropin-releasing hormone agonist (mGnRHa) into poly(lactic-co-glycolic acid) (PLGA) microparticles on the sex steroid levels and aspects of artificial reproduction of pikeperch. Fish were divided into four groups and injected with 20 µg mGnRHa/kg, 5-day release microparticles encapsulated with 5 µg GnRHa/kg BW (PLGA 5), 20 µg GnRHa/kg (PLGA 20), or 1 mL/kg 0.9% NaCl (control). Cumulative percentage ovulation was 100% in the PLGA 5 group, significantly higher than in other tested groups. No differences among groups were observed in latency or fecundity. The level of 11-ketotestosterone (11-KT) peaked at 40 h post-injection, and was sustained during ovulation, in all treated groups. The 17β-estradiol (E2) concentration increased in the mGnRHa-only group immediately after hormone injection, while both PLGA groups showed a reduction in E2 after injection, continuing to decrease until ovulation. A low dose of mGnRHa in PLGA microparticles significantly improves induction of ovulation and results in acceptable reproductive performance, which may positively affect pikeperch production under controlled conditions.https://www.mdpi.com/2076-2615/12/2/208aquacultureGnRHainduced ovulationreproductive dysfunctionsustained drug release |
| spellingShingle | Jindřiška Knowles Jakub Vysloužil Tomáš Policar Sylvain Milla Martina Holická Peter Podhorec Spawning Performance and Sex Steroid Levels in Female Pikeperch <i>Sander lucioperca</i> Treated with Poly(lactic-co-glycolic acid) Microparticles Animals aquaculture GnRHa induced ovulation reproductive dysfunction sustained drug release |
| title | Spawning Performance and Sex Steroid Levels in Female Pikeperch <i>Sander lucioperca</i> Treated with Poly(lactic-co-glycolic acid) Microparticles |
| title_full | Spawning Performance and Sex Steroid Levels in Female Pikeperch <i>Sander lucioperca</i> Treated with Poly(lactic-co-glycolic acid) Microparticles |
| title_fullStr | Spawning Performance and Sex Steroid Levels in Female Pikeperch <i>Sander lucioperca</i> Treated with Poly(lactic-co-glycolic acid) Microparticles |
| title_full_unstemmed | Spawning Performance and Sex Steroid Levels in Female Pikeperch <i>Sander lucioperca</i> Treated with Poly(lactic-co-glycolic acid) Microparticles |
| title_short | Spawning Performance and Sex Steroid Levels in Female Pikeperch <i>Sander lucioperca</i> Treated with Poly(lactic-co-glycolic acid) Microparticles |
| title_sort | spawning performance and sex steroid levels in female pikeperch i sander lucioperca i treated with poly lactic co glycolic acid microparticles |
| topic | aquaculture GnRHa induced ovulation reproductive dysfunction sustained drug release |
| url | https://www.mdpi.com/2076-2615/12/2/208 |
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