Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus
Foot-and-mouth disease virus (FMDV) is endemic in many parts of the world. Vaccination is an important control measure, limits viral spread, and can help to eradicate the disease. However, vaccination programs are cost-intensive because of the short shelf life of vaccines and the need for frequent r...
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| Format: | Article |
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MDPI AG
2019-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/6/511 |
| _version_ | 1818352569289801728 |
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| author | Veronika Dill Janike Ehret Aline Zimmer Martin Beer Michael Eschbaumer |
| author_facet | Veronika Dill Janike Ehret Aline Zimmer Martin Beer Michael Eschbaumer |
| author_sort | Veronika Dill |
| collection | DOAJ |
| description | Foot-and-mouth disease virus (FMDV) is endemic in many parts of the world. Vaccination is an important control measure, limits viral spread, and can help to eradicate the disease. However, vaccination programs are cost-intensive because of the short shelf life of vaccines and the need for frequent re-vaccination. Animal-component-free (ACF) or chemically defined media (CDM) at high cell densities are a promising approach for the production of inexpensive high-quality vaccines, but the occurrence of cell density effects has been reported for various virus-cell systems in vaccine production. For FMDV, the use of CDM or ACF media for vaccine production has not been studied and no information about cell density effects is available. This work describes the propagation of FMDV in ACF or in CDM. Cells were grown at increasing cell densities and either 100% media exchange or addition of 30% fresh media was performed before infection with FMDV. Increasing cell densities reduced the viral titer and increased yield variability in all media except BHK300G. This effect can be mitigated by performing a 100% media exchange before infection or when using the controlled environment of a bioreactor. The media composition and also a fragile relationship between virus and cell metabolism seem to be causal for that phenomenon. |
| first_indexed | 2024-12-13T18:55:43Z |
| format | Article |
| id | doaj.art-d5de06042e974d5a83d1c55aaf9eda29 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-13T18:55:43Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-d5de06042e974d5a83d1c55aaf9eda292022-12-21T23:34:48ZengMDPI AGViruses1999-49152019-06-0111651110.3390/v11060511v11060511Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease VirusVeronika Dill0Janike Ehret1Aline Zimmer2Martin Beer3Michael Eschbaumer4Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald–Insel Riems, GermanyMerck KGaA, Merck Life Sciences, Upstream R&D, Frankfurter Straße 250, 64293 Darmstadt, GermanyMerck KGaA, Merck Life Sciences, Upstream R&D, Frankfurter Straße 250, 64293 Darmstadt, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald–Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald–Insel Riems, GermanyFoot-and-mouth disease virus (FMDV) is endemic in many parts of the world. Vaccination is an important control measure, limits viral spread, and can help to eradicate the disease. However, vaccination programs are cost-intensive because of the short shelf life of vaccines and the need for frequent re-vaccination. Animal-component-free (ACF) or chemically defined media (CDM) at high cell densities are a promising approach for the production of inexpensive high-quality vaccines, but the occurrence of cell density effects has been reported for various virus-cell systems in vaccine production. For FMDV, the use of CDM or ACF media for vaccine production has not been studied and no information about cell density effects is available. This work describes the propagation of FMDV in ACF or in CDM. Cells were grown at increasing cell densities and either 100% media exchange or addition of 30% fresh media was performed before infection with FMDV. Increasing cell densities reduced the viral titer and increased yield variability in all media except BHK300G. This effect can be mitigated by performing a 100% media exchange before infection or when using the controlled environment of a bioreactor. The media composition and also a fragile relationship between virus and cell metabolism seem to be causal for that phenomenon.https://www.mdpi.com/1999-4915/11/6/511foot-and-mouth disease viruscell densityanimal-component-free mediachemically defined mediaantifoamsuspension cells |
| spellingShingle | Veronika Dill Janike Ehret Aline Zimmer Martin Beer Michael Eschbaumer Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus Viruses foot-and-mouth disease virus cell density animal-component-free media chemically defined media antifoam suspension cells |
| title | Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus |
| title_full | Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus |
| title_fullStr | Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus |
| title_full_unstemmed | Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus |
| title_short | Cell Density Effects in Different Cell Culture Media and Their Impact on the Propagation of Foot-And-Mouth Disease Virus |
| title_sort | cell density effects in different cell culture media and their impact on the propagation of foot and mouth disease virus |
| topic | foot-and-mouth disease virus cell density animal-component-free media chemically defined media antifoam suspension cells |
| url | https://www.mdpi.com/1999-4915/11/6/511 |
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