A multiscale approach to blast neurotrauma modeling: Part I - Development of novel test devices for in vivo and in vitro blast injury models
The loading conditions used in some current in vivo and in vitro blast-induced neurotrauma models may not be representative of real-world blast conditions. To address these limitations, we developed a compressed-gas driven shock tube with different driven lengths that can generate Friedlander-type b...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2012-03-01
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| Series: | Frontiers in Neurology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fneur.2012.00046/full |
| _version_ | 1811306949413699584 |
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| author | Matthew B Panzer Kyle A Matthews Allen W Yu Barclay eMorrison David F Meaney Cameron R Bass |
| author_facet | Matthew B Panzer Kyle A Matthews Allen W Yu Barclay eMorrison David F Meaney Cameron R Bass |
| author_sort | Matthew B Panzer |
| collection | DOAJ |
| description | The loading conditions used in some current in vivo and in vitro blast-induced neurotrauma models may not be representative of real-world blast conditions. To address these limitations, we developed a compressed-gas driven shock tube with different driven lengths that can generate Friedlander-type blasts. The shock tube can generate overpressures up to 650 kPa with durations between 0.3 and 1.1 ms using compressed helium driver gas, and peak overpressures up to 450 kPa with durations between 0.6 and 3 ms using compressed nitrogen. This device is used for short duration blast overpressure loading for small animal in vivo injury models, and contrasts the more frequently used long duration/high impulse blast overpressures in the literature. We also developed a new apparatus that is used with the shock tube to recreate the in vivo intracranial overpressure response for loading in vitro culture preparations. The receiver device surrounds the culture with materials of similar impedance to facilitate the propagation of a single overpressure pulse through the tissue. This method prevents pressure waves reflecting off the tissue that can cause unrealistic deformation and injury. The receiver performance was characterized using the longest helium-driven shock tube, and produced in-fluid overpressures up to 1500 kPa at the location where a culture would be placed. This response was well correlated with the overpressure conditions from the shock tube (R2 = 0.97). Finite element models of the shock tube and receiver were developed and validated to better elucidate the mechanics of this methodology. A demonstration exposing a culture to the loading conditions created by this system suggest tissue strains less than 5% for all pressure levels simulated, which was well below functional deficit thresholds for strain rates less than 50 s-1. This novel system is not limited to a specific type of culture model and can be modified to reproduce more complex pressure pulses. |
| first_indexed | 2024-04-13T08:55:57Z |
| format | Article |
| id | doaj.art-d5df572a33574fbaa7851bc4349aeb88 |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-04-13T08:55:57Z |
| publishDate | 2012-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-d5df572a33574fbaa7851bc4349aeb882022-12-22T02:53:19ZengFrontiers Media S.A.Frontiers in Neurology1664-22952012-03-01310.3389/fneur.2012.0004618331A multiscale approach to blast neurotrauma modeling: Part I - Development of novel test devices for in vivo and in vitro blast injury modelsMatthew B Panzer0Kyle A Matthews1Allen W Yu2Barclay eMorrison3David F Meaney4Cameron R Bass5Duke UniversityDuke UniversityDuke UniversityColumbia UniversityUniversity of PennsylvaniaDuke UniversityThe loading conditions used in some current in vivo and in vitro blast-induced neurotrauma models may not be representative of real-world blast conditions. To address these limitations, we developed a compressed-gas driven shock tube with different driven lengths that can generate Friedlander-type blasts. The shock tube can generate overpressures up to 650 kPa with durations between 0.3 and 1.1 ms using compressed helium driver gas, and peak overpressures up to 450 kPa with durations between 0.6 and 3 ms using compressed nitrogen. This device is used for short duration blast overpressure loading for small animal in vivo injury models, and contrasts the more frequently used long duration/high impulse blast overpressures in the literature. We also developed a new apparatus that is used with the shock tube to recreate the in vivo intracranial overpressure response for loading in vitro culture preparations. The receiver device surrounds the culture with materials of similar impedance to facilitate the propagation of a single overpressure pulse through the tissue. This method prevents pressure waves reflecting off the tissue that can cause unrealistic deformation and injury. The receiver performance was characterized using the longest helium-driven shock tube, and produced in-fluid overpressures up to 1500 kPa at the location where a culture would be placed. This response was well correlated with the overpressure conditions from the shock tube (R2 = 0.97). Finite element models of the shock tube and receiver were developed and validated to better elucidate the mechanics of this methodology. A demonstration exposing a culture to the loading conditions created by this system suggest tissue strains less than 5% for all pressure levels simulated, which was well below functional deficit thresholds for strain rates less than 50 s-1. This novel system is not limited to a specific type of culture model and can be modified to reproduce more complex pressure pulses.http://journal.frontiersin.org/Journal/10.3389/fneur.2012.00046/fullTraumatic Brain Injuryblast injuryin vitro modelin vivo modelshock tube |
| spellingShingle | Matthew B Panzer Kyle A Matthews Allen W Yu Barclay eMorrison David F Meaney Cameron R Bass A multiscale approach to blast neurotrauma modeling: Part I - Development of novel test devices for in vivo and in vitro blast injury models Frontiers in Neurology Traumatic Brain Injury blast injury in vitro model in vivo model shock tube |
| title | A multiscale approach to blast neurotrauma modeling: Part I - Development of novel test devices for in vivo and in vitro blast injury models |
| title_full | A multiscale approach to blast neurotrauma modeling: Part I - Development of novel test devices for in vivo and in vitro blast injury models |
| title_fullStr | A multiscale approach to blast neurotrauma modeling: Part I - Development of novel test devices for in vivo and in vitro blast injury models |
| title_full_unstemmed | A multiscale approach to blast neurotrauma modeling: Part I - Development of novel test devices for in vivo and in vitro blast injury models |
| title_short | A multiscale approach to blast neurotrauma modeling: Part I - Development of novel test devices for in vivo and in vitro blast injury models |
| title_sort | multiscale approach to blast neurotrauma modeling part i development of novel test devices for in vivo and in vitro blast injury models |
| topic | Traumatic Brain Injury blast injury in vitro model in vivo model shock tube |
| url | http://journal.frontiersin.org/Journal/10.3389/fneur.2012.00046/full |
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