Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts
Aspergillus niger is a very destructive pathogen causing severe peanut root rot, especially in the seeding stage of peanuts (Arachis hypogaea), and often leading to the death of the plant. Protein lysine 2-hydroxyisobutyrylation (Khib) is a newly detected post-translational modification identified i...
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| Format: | Article |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.719337/full |
| _version_ | 1819124499201654784 |
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| author | Manlin Xu Xia Zhang Jing Yu Zhiqing Guo Ying Li Xinying Song Kang He Guowei Li Yucheng Chi |
| author_facet | Manlin Xu Xia Zhang Jing Yu Zhiqing Guo Ying Li Xinying Song Kang He Guowei Li Yucheng Chi |
| author_sort | Manlin Xu |
| collection | DOAJ |
| description | Aspergillus niger is a very destructive pathogen causing severe peanut root rot, especially in the seeding stage of peanuts (Arachis hypogaea), and often leading to the death of the plant. Protein lysine 2-hydroxyisobutyrylation (Khib) is a newly detected post-translational modification identified in several species. In this study, we identified 5041 Khib sites on 1,453 modified proteins in A. niger. Compared with five other species, A. niger has conserved and novel proteins. Bioinformatics analysis showed that Khib proteins are widely distributed in A. niger and are involved in many biological processes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that Khib proteins were significantly enriched in many cellular compartments and pathways, such as ribosomes and proteasome subunits. A total of 223 Khib proteins were part of the PPI network, thus, suggesting that Khib proteins are associated with a large range of protein interactions and diverse pathways in the life processes of A. niger. Several identified proteins are involved in pathogenesis regulation. Our research provides the first comprehensive report of Khib and an extensive database for potential functional studies on Khib proteins in this economically important fungus. |
| first_indexed | 2024-12-22T07:25:13Z |
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| id | doaj.art-d5e059e3ff584d40a72ebd635205e95a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T07:25:13Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-d5e059e3ff584d40a72ebd635205e95a2022-12-21T18:34:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-08-011210.3389/fmicb.2021.719337719337Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in PeanutsManlin Xu0Xia Zhang1Jing Yu2Zhiqing Guo3Ying Li4Xinying Song5Kang He6Guowei Li7Yucheng Chi8Shandong Peanut Research Institute, Qingdao, ChinaShandong Peanut Research Institute, Qingdao, ChinaShandong Peanut Research Institute, Qingdao, ChinaShandong Peanut Research Institute, Qingdao, ChinaShandong Peanut Research Institute, Qingdao, ChinaShandong Peanut Research Institute, Qingdao, ChinaShandong Peanut Research Institute, Qingdao, ChinaInstitute of Crop Germplasm Resources, SAAS, Jinan, ChinaShandong Peanut Research Institute, Qingdao, ChinaAspergillus niger is a very destructive pathogen causing severe peanut root rot, especially in the seeding stage of peanuts (Arachis hypogaea), and often leading to the death of the plant. Protein lysine 2-hydroxyisobutyrylation (Khib) is a newly detected post-translational modification identified in several species. In this study, we identified 5041 Khib sites on 1,453 modified proteins in A. niger. Compared with five other species, A. niger has conserved and novel proteins. Bioinformatics analysis showed that Khib proteins are widely distributed in A. niger and are involved in many biological processes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that Khib proteins were significantly enriched in many cellular compartments and pathways, such as ribosomes and proteasome subunits. A total of 223 Khib proteins were part of the PPI network, thus, suggesting that Khib proteins are associated with a large range of protein interactions and diverse pathways in the life processes of A. niger. Several identified proteins are involved in pathogenesis regulation. Our research provides the first comprehensive report of Khib and an extensive database for potential functional studies on Khib proteins in this economically important fungus.https://www.frontiersin.org/articles/10.3389/fmicb.2021.719337/fullAspergillus nigerproteomepost-translational modificationspathogenesislysine 2-hydroxyisobutyrylation |
| spellingShingle | Manlin Xu Xia Zhang Jing Yu Zhiqing Guo Ying Li Xinying Song Kang He Guowei Li Yucheng Chi Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts Frontiers in Microbiology Aspergillus niger proteome post-translational modifications pathogenesis lysine 2-hydroxyisobutyrylation |
| title | Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts |
| title_full | Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts |
| title_fullStr | Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts |
| title_full_unstemmed | Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts |
| title_short | Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts |
| title_sort | proteome wide analysis of lysine 2 hydroxyisobutyrylation in aspergillus niger in peanuts |
| topic | Aspergillus niger proteome post-translational modifications pathogenesis lysine 2-hydroxyisobutyrylation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.719337/full |
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