Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins
Red blood cell (RBC) deformability, expressing their ability to change their shape, allows them to minimize their resistance to flow and optimize oxygen delivery to the tissues. RBC with reduced deformability may lead to increased vascular resistance, capillary occlusion, and impaired perfusion and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/16/12755 |
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| author | Gregory Barshtein Alexander Gural Dan Arbell Refael Barkan Leonid Livshits Ivana Pajic-Lijakovic Saul Yedgar |
| author_facet | Gregory Barshtein Alexander Gural Dan Arbell Refael Barkan Leonid Livshits Ivana Pajic-Lijakovic Saul Yedgar |
| author_sort | Gregory Barshtein |
| collection | DOAJ |
| description | Red blood cell (RBC) deformability, expressing their ability to change their shape, allows them to minimize their resistance to flow and optimize oxygen delivery to the tissues. RBC with reduced deformability may lead to increased vascular resistance, capillary occlusion, and impaired perfusion and oxygen delivery. A reduction in deformability, as occurs during RBC physiological aging and under blood storage, is implicated in the pathophysiology of diverse conditions with circulatory disorders and anemias. The change in RBC deformability is associated with metabolic and structural alterations, mostly uncharacterized. To bridge this gap, we analyzed the membrane protein levels, using mass spectroscopy, of RBC with varying deformability determined by image analysis. In total, 752 membrane proteins were identified. However, deformability was positively correlated with the level of only fourteen proteins, with a highly significant inter-correlation between them. These proteins are involved in membrane rafting and/or the membrane–cytoskeleton linkage. These findings suggest that the reduction of deformability is a programmed (not arbitrary) process of remodeling and shedding of membrane fragments, possibly mirroring the formation of extracellular vesicles. The highly significant inter-correlation between the deformability-expressing proteins infers that the cell deformability can be assessed by determining the level of a few, possibly one, of them. |
| first_indexed | 2024-03-10T23:52:48Z |
| format | Article |
| id | doaj.art-d5e5c76b31da4e6b99d18fef0681d439 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:52:48Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d5e5c76b31da4e6b99d18fef0681d4392023-11-19T01:28:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124161275510.3390/ijms241612755Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane ProteinsGregory Barshtein0Alexander Gural1Dan Arbell2Refael Barkan3Leonid Livshits4Ivana Pajic-Lijakovic5Saul Yedgar6Department of Biochemistry, The Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112001, IsraelBlood Bank, Hadassah University Hospital, Jerusalem 9112001, IsraelPediatric Surgery, Hadassah University Hospital, Jerusalem 9112001, IsraelDepartment of Digital Medical Technologies, Holon Institute of Technology, Holon 5810201, IsraelRed Blood Cell Research Group, Vetsuisse Faculty, Institute of Veterinary Physiology, University of Zurich, 8057 Zürich, SwitzerlandDepartment of Chemical Engineering, University of Belgrade, 11120 Belgrade, SerbiaDepartment of Biochemistry, The Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112001, IsraelRed blood cell (RBC) deformability, expressing their ability to change their shape, allows them to minimize their resistance to flow and optimize oxygen delivery to the tissues. RBC with reduced deformability may lead to increased vascular resistance, capillary occlusion, and impaired perfusion and oxygen delivery. A reduction in deformability, as occurs during RBC physiological aging and under blood storage, is implicated in the pathophysiology of diverse conditions with circulatory disorders and anemias. The change in RBC deformability is associated with metabolic and structural alterations, mostly uncharacterized. To bridge this gap, we analyzed the membrane protein levels, using mass spectroscopy, of RBC with varying deformability determined by image analysis. In total, 752 membrane proteins were identified. However, deformability was positively correlated with the level of only fourteen proteins, with a highly significant inter-correlation between them. These proteins are involved in membrane rafting and/or the membrane–cytoskeleton linkage. These findings suggest that the reduction of deformability is a programmed (not arbitrary) process of remodeling and shedding of membrane fragments, possibly mirroring the formation of extracellular vesicles. The highly significant inter-correlation between the deformability-expressing proteins infers that the cell deformability can be assessed by determining the level of a few, possibly one, of them.https://www.mdpi.com/1422-0067/24/16/12755red blood cellsRBC deformabilitymembrane proteinslipid raftsmembrane vesiclesmembrane remodeling |
| spellingShingle | Gregory Barshtein Alexander Gural Dan Arbell Refael Barkan Leonid Livshits Ivana Pajic-Lijakovic Saul Yedgar Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins International Journal of Molecular Sciences red blood cells RBC deformability membrane proteins lipid rafts membrane vesicles membrane remodeling |
| title | Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins |
| title_full | Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins |
| title_fullStr | Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins |
| title_full_unstemmed | Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins |
| title_short | Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins |
| title_sort | red blood cell deformability is expressed by a set of interrelated membrane proteins |
| topic | red blood cells RBC deformability membrane proteins lipid rafts membrane vesicles membrane remodeling |
| url | https://www.mdpi.com/1422-0067/24/16/12755 |
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