Detection of Protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an Environmental Education Center in southern Brazil
The presence of coliforms, Mastadenovirus (AdV), and Canine protoparvovirus (CPV) in an Environmental Education Center, which contains city kennel, was investigated. Surface water and wastewater, dogs’ stool, and lettuce samples were collected from the irrigation system in both periods October 2017...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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IWA Publishing
2022-12-01
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| Series: | Water Policy |
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| Online Access: | http://wpol.iwaponline.com/content/24/12/1827 |
| _version_ | 1797903479323230208 |
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| author | Leila Elisa Gartner Meriane Demoliner Viviane Girardi Kelen Gras de Oliveira Fernanda Gil de Souza Andréia Henzel |
| author_facet | Leila Elisa Gartner Meriane Demoliner Viviane Girardi Kelen Gras de Oliveira Fernanda Gil de Souza Andréia Henzel |
| author_sort | Leila Elisa Gartner |
| collection | DOAJ |
| description | The presence of coliforms, Mastadenovirus (AdV), and Canine protoparvovirus (CPV) in an Environmental Education Center, which contains city kennel, was investigated. Surface water and wastewater, dogs’ stool, and lettuce samples were collected from the irrigation system in both periods October 2017 and February 2018. Water samples were analyzed for the presence of human AdV (HAdV) and canine AdV (CAV), CPV, total coliforms (TC), and Escherichia coli; lettuce for AdV; and dog stool for CAV and CPV. Sixty-seven samples were analyzed: dog stool (n = 33); water (n = 10), and root, stem, and leaf (n = 24) originated from eight lettuce samples. Coliforms and E. coli were tested by Colilert and viruses by PCR assays, respectively; and the amplicon was sequenced. E. coli, TC, and viruses were detected in both collections. Viruses were detected in 34.3% of samples, of which HAdV-C was present in 13% of water samples; HAdV-E in 8.7% (water and lettuce); CAV-1 in 13% (dog stool and water); CPV-2a in 56.5% (dog stool). Raccoon CPV-like and AdV not characterized were detected in one wastewater samples and one dog stool, respectively. To our knowledge, this is the first description of the presence of Raccoon CPV-like in water and HAdV-E in lettuce in Southern Brazil.
HIGHLIGHTS
Protoparvovirus in wastewater in Brazil is detected.;
Raccon CPV-like can represent a potential transmission interspecies.;
HAdV-E in lettuce from an irrigation system – Southern Brazil – is presented.;
Lettuce diseases are likely waterborne viral diseases.; |
| first_indexed | 2024-04-10T09:33:35Z |
| format | Article |
| id | doaj.art-d5e7107d51c94768bb31ce9f88f7345b |
| institution | Directory Open Access Journal |
| issn | 1366-7017 1996-9759 |
| language | English |
| last_indexed | 2024-04-10T09:33:35Z |
| publishDate | 2022-12-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Water Policy |
| spelling | doaj.art-d5e7107d51c94768bb31ce9f88f7345b2023-02-18T09:54:37ZengIWA PublishingWater Policy1366-70171996-97592022-12-0124121827184110.2166/wp.2022.062062Detection of Protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an Environmental Education Center in southern BrazilLeila Elisa Gartner0Meriane Demoliner1Viviane Girardi2Kelen Gras de Oliveira3Fernanda Gil de Souza4Andréia Henzel5 Graduada em Ciências Biológicas pela Universidade Feevale, Novo Hamburgo, Rio Grande do Sul (RS), Brazil Laboratório de Microbiologia Molecular (LMM), Instituto de Ciência da Saúde (ICS), Universidade Feevale, Novo Hamburgo, RS, Brazil Laboratório de Microbiologia Molecular (LMM), Instituto de Ciência da Saúde (ICS), Universidade Feevale, Novo Hamburgo, RS, Brazil Laboratório de Microbiologia Molecular (LMM), Instituto de Ciência da Saúde (ICS), Universidade Feevale, Novo Hamburgo, RS, Brazil Laboratório de Microbiologia Molecular (LMM), Instituto de Ciência da Saúde (ICS), Universidade Feevale, Novo Hamburgo, RS, Brazil Veterinarian, Doctor, Virologist, Research and Development (R&D) Department, Merck Sharp & Dohme (MSD) LATAM. Montes Claros, Minas Gerais, Brazil The presence of coliforms, Mastadenovirus (AdV), and Canine protoparvovirus (CPV) in an Environmental Education Center, which contains city kennel, was investigated. Surface water and wastewater, dogs’ stool, and lettuce samples were collected from the irrigation system in both periods October 2017 and February 2018. Water samples were analyzed for the presence of human AdV (HAdV) and canine AdV (CAV), CPV, total coliforms (TC), and Escherichia coli; lettuce for AdV; and dog stool for CAV and CPV. Sixty-seven samples were analyzed: dog stool (n = 33); water (n = 10), and root, stem, and leaf (n = 24) originated from eight lettuce samples. Coliforms and E. coli were tested by Colilert and viruses by PCR assays, respectively; and the amplicon was sequenced. E. coli, TC, and viruses were detected in both collections. Viruses were detected in 34.3% of samples, of which HAdV-C was present in 13% of water samples; HAdV-E in 8.7% (water and lettuce); CAV-1 in 13% (dog stool and water); CPV-2a in 56.5% (dog stool). Raccoon CPV-like and AdV not characterized were detected in one wastewater samples and one dog stool, respectively. To our knowledge, this is the first description of the presence of Raccoon CPV-like in water and HAdV-E in lettuce in Southern Brazil. HIGHLIGHTS Protoparvovirus in wastewater in Brazil is detected.; Raccon CPV-like can represent a potential transmission interspecies.; HAdV-E in lettuce from an irrigation system – Southern Brazil – is presented.; Lettuce diseases are likely waterborne viral diseases.;http://wpol.iwaponline.com/content/24/12/1827cav-1coliformshadv-c and -ekennelraccoon cpv-like |
| spellingShingle | Leila Elisa Gartner Meriane Demoliner Viviane Girardi Kelen Gras de Oliveira Fernanda Gil de Souza Andréia Henzel Detection of Protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an Environmental Education Center in southern Brazil Water Policy cav-1 coliforms hadv-c and -e kennel raccoon cpv-like |
| title | Detection of Protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an Environmental Education Center in southern Brazil |
| title_full | Detection of Protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an Environmental Education Center in southern Brazil |
| title_fullStr | Detection of Protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an Environmental Education Center in southern Brazil |
| title_full_unstemmed | Detection of Protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an Environmental Education Center in southern Brazil |
| title_short | Detection of Protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an Environmental Education Center in southern Brazil |
| title_sort | detection of protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an environmental education center in southern brazil |
| topic | cav-1 coliforms hadv-c and -e kennel raccoon cpv-like |
| url | http://wpol.iwaponline.com/content/24/12/1827 |
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