FtbZIP12 Positively Regulates Responses to Osmotic Stress in Tartary Buckwheat

FtbZIP12 Positively Regulates Responses to Osmotic Stress in Tartary Buckwheat

<i>ABFs</i> play a key role in regulating plant osmotic stress. However, in Tartary buckwheat, data on the role of <i>ABF</i> genes in osmotic stress remain limited and its associated mechanism in osmoregulation remain nebulous. Herein, a novel <i>ABF</i> family i...

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Bibliographic Details
Main Authors: Wenfeng Weng, Xiang Lu, Meiliang Zhou, Anjing Gao, Xin Yao, Yong Tang, Weijiao Wu, Chao Ma, Qing Bai, Ruiqi Xiong, Jingjun Ruan
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Fagopyrum tataricum</i>
<i>FtbZIP12</i>
osmotic stress
<i>Arabidopsis thaliana</i>
plant resistance
Online Access:https://www.mdpi.com/1422-0067/23/21/13072
Description
Summary:<i>ABFs</i> play a key role in regulating plant osmotic stress. However, in Tartary buckwheat, data on the role of <i>ABF</i> genes in osmotic stress remain limited and its associated mechanism in osmoregulation remain nebulous. Herein, a novel <i>ABF</i> family in Tartary buckwheat, <i>FtbZIP12</i>, was cloned and characterized. <i>FtbZIP12</i> is a transcriptional activator located in the nucleus; its expression is induced by NaCl, mannitol, and abscisic acid (ABA). Atopic expression of <i>FtbZIP12</i> in <i>Arabidopsis</i> promoted seed germination, reduced damage to primary roots, and improved the tolerance of seedlings to osmotic stress. The quantitative realtime polymerase chain reaction (RT-qPCR) results showed that the expressions of the typical genes related to stress, the SOS pathway, and the proline synthesis pathway in <i>Arabidopsis</i> were significantly (<i>p</i> < 0.05) upregulated under osmotic stress. <i>FtbZIP12</i> improved the osmotic pressure resistance by reducing the damage caused by reactive oxygen species to plants and maintained plant homeostasis by upregulating the expression of genes related to stress, osmotic regulation, and ion homeostasis. This study identified a key candidate gene for understanding the mechanism underlying osmotic-stress-regulated function in Tartary buckwheat, thereby providing a theoretical basis for improving its yield and quality.
ISSN:1661-6596
1422-0067

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