In vitro evaluation of copper tolerance and accumulation in Populus nigra
Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 1...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641700014V.pdf |
| Summary: | Phytoextraction is an efficient and cheap way to extract copper from soils in
riparian zones. In this work five genotypes of the endangered tree species
Populus nigra L. were tested for their copper tolerance and accumulation in
vitro when cultivated on media with three Cu concentrations: 10-3, 10-4 and
10-7 M (buffered with citric acid/Na-citrate buffer, pH 3 before
sterilization). After five-weeks cultivation of rooted shoots, the highest
increases in morphological and biomass parameters were observed at 10-7 M
Cu2+. As the medium with 10-3 M Cu2+ exhibited a toxic effect, the effect of
10-4 M Cu2+ and pH 3 was used for further genotype evaluation. According to
the measured morphological and parameters of photosynthetic pigment
contents, the best performance was achieved by the genotype Populus nigra
cl. DN3. The highest copper accumulation on the same medium was achieved by
genotype Populus nigra cl. BN5. The obtained data point to the considerable
potential of the applied method in the evaluation of Populus nigra genotypes
for use in projects of copper phytoextraction. [Project of the Serbian
Ministry of Education, Science and Technological Development, Grant no. III
43007: Studying climate change and its influence on the environment:
impacts, adaptation and mitigation] |
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| ISSN: | 0354-4664 1821-4339 |