Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration
Abstract Background Older adults are more prone to develop systemic dehydration. Systemic dehydration has implications for vocal fold biology by affecting gene and protein expression. The objective of this study was to quantify vocal fold protein changes between two age groups and hydration status,...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-022-08975-x |
| _version_ | 1797986884882792448 |
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| author | Naila C. do Nascimento Taylor W. Bailey Andrea P. Santos Chenwei Duan Rodrigo Mohallem Jackeline Franco Uma K. Aryal Jun Xie Abigail Cox M. Preeti Sivasankar |
| author_facet | Naila C. do Nascimento Taylor W. Bailey Andrea P. Santos Chenwei Duan Rodrigo Mohallem Jackeline Franco Uma K. Aryal Jun Xie Abigail Cox M. Preeti Sivasankar |
| author_sort | Naila C. do Nascimento |
| collection | DOAJ |
| description | Abstract Background Older adults are more prone to develop systemic dehydration. Systemic dehydration has implications for vocal fold biology by affecting gene and protein expression. The objective of this study was to quantify vocal fold protein changes between two age groups and hydration status, and to investigate the interaction of age and hydration status on protein expression, which has not been investigated in the context of vocal folds before. Comparative proteomics was used to analyze the vocal fold proteome of 6.5-month-old and > 3-year-old rabbits subjected to water ad libitum or water volume restriction protocol. Results Young and older adult rabbits (n = 22) were either euhydrated (water ad libitum) or dehydrated by water volume restriction. Dehydration was confirmed by body weight loss of − 5.4% and − 4.6% in young and older groups, respectively, and a 1.7-fold increase of kidney renin gene expression in the young rabbits. LC-MS/MS identified 2286 proteins in the rabbit vocal folds of young and older adult rabbits combined. Of these, 177, 169, and 81 proteins were significantly (p ≤ 0.05) affected by age, hydration status, or the interaction of both factors, respectively. Analysis of the interaction effect revealed 32 proteins with opposite change patterns after dehydration between older and young rabbit vocal folds, while 31 proteins were differentially regulated only in the older adult rabbits and ten only in the young rabbits in response to systemic dehydration. The magnitude of changes for either up or downregulated proteins was higher in the older rabbits. These proteins are predominantly related to structural components of the extracellular matrix and muscle layer, suggesting a disturbance in the viscoelastic properties of aging vocal fold tissue, especially when subjected to systemic dehydration. Conclusions Water restriction is a laboratory protocol to assess systemic dehydration-related changes in the vocal fold tissue that is translatable to human subjects. Our findings showed a higher number of proteins differentially regulated with a greater magnitude of change in the vocal folds of older adult rabbits in the presence of systemic dehydration compared to younger rabbits. The association of these proteins with vocal fold structure and biomechanical properties suggests that older human subjects may be more vulnerable to the effects of systemic dehydration on vocal function. The clinical implications of these protein changes warrant more investigation, but age should be taken into consideration when evaluating vocal treatment recommendations that interfere with body fluid balance. |
| first_indexed | 2024-04-11T07:38:54Z |
| format | Article |
| id | doaj.art-d5f3f734fe804a78b81712c9fc2e1e35 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-11T07:38:54Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-d5f3f734fe804a78b81712c9fc2e1e352022-12-22T04:36:38ZengBMCBMC Genomics1471-21642022-11-0123112210.1186/s12864-022-08975-xProteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydrationNaila C. do Nascimento0Taylor W. Bailey1Andrea P. Santos2Chenwei Duan3Rodrigo Mohallem4Jackeline Franco5Uma K. Aryal6Jun Xie7Abigail Cox8M. Preeti Sivasankar9Department of Speech, Language, and Hearing Sciences, Purdue UniversityDepartment of Comparative Pathobiology, Purdue UniversityDepartment of Comparative Pathobiology, Purdue UniversityWeldon School of Biomedical Engineering, Purdue UniversityDepartment of Comparative Pathobiology, Purdue UniversityPurdue Proteomics Facility, Bindley Bioscience Center, Discovery Park, Purdue UniversityDepartment of Comparative Pathobiology, Purdue UniversityDepartment of Statistics, Purdue UniversityDepartment of Comparative Pathobiology, Purdue UniversityDepartment of Speech, Language, and Hearing Sciences, Purdue UniversityAbstract Background Older adults are more prone to develop systemic dehydration. Systemic dehydration has implications for vocal fold biology by affecting gene and protein expression. The objective of this study was to quantify vocal fold protein changes between two age groups and hydration status, and to investigate the interaction of age and hydration status on protein expression, which has not been investigated in the context of vocal folds before. Comparative proteomics was used to analyze the vocal fold proteome of 6.5-month-old and > 3-year-old rabbits subjected to water ad libitum or water volume restriction protocol. Results Young and older adult rabbits (n = 22) were either euhydrated (water ad libitum) or dehydrated by water volume restriction. Dehydration was confirmed by body weight loss of − 5.4% and − 4.6% in young and older groups, respectively, and a 1.7-fold increase of kidney renin gene expression in the young rabbits. LC-MS/MS identified 2286 proteins in the rabbit vocal folds of young and older adult rabbits combined. Of these, 177, 169, and 81 proteins were significantly (p ≤ 0.05) affected by age, hydration status, or the interaction of both factors, respectively. Analysis of the interaction effect revealed 32 proteins with opposite change patterns after dehydration between older and young rabbit vocal folds, while 31 proteins were differentially regulated only in the older adult rabbits and ten only in the young rabbits in response to systemic dehydration. The magnitude of changes for either up or downregulated proteins was higher in the older rabbits. These proteins are predominantly related to structural components of the extracellular matrix and muscle layer, suggesting a disturbance in the viscoelastic properties of aging vocal fold tissue, especially when subjected to systemic dehydration. Conclusions Water restriction is a laboratory protocol to assess systemic dehydration-related changes in the vocal fold tissue that is translatable to human subjects. Our findings showed a higher number of proteins differentially regulated with a greater magnitude of change in the vocal folds of older adult rabbits in the presence of systemic dehydration compared to younger rabbits. The association of these proteins with vocal fold structure and biomechanical properties suggests that older human subjects may be more vulnerable to the effects of systemic dehydration on vocal function. The clinical implications of these protein changes warrant more investigation, but age should be taken into consideration when evaluating vocal treatment recommendations that interfere with body fluid balance.https://doi.org/10.1186/s12864-022-08975-xYoungAgingLarynxVocal foldsWater restrictionDehydration |
| spellingShingle | Naila C. do Nascimento Taylor W. Bailey Andrea P. Santos Chenwei Duan Rodrigo Mohallem Jackeline Franco Uma K. Aryal Jun Xie Abigail Cox M. Preeti Sivasankar Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration BMC Genomics Young Aging Larynx Vocal folds Water restriction Dehydration |
| title | Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration |
| title_full | Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration |
| title_fullStr | Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration |
| title_full_unstemmed | Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration |
| title_short | Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration |
| title_sort | proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration |
| topic | Young Aging Larynx Vocal folds Water restriction Dehydration |
| url | https://doi.org/10.1186/s12864-022-08975-x |
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