Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate
Extracellular matrix remodeling plays a pivotal role during mesenchyme patterning into different lineages. Tension exerted from cell membrane receptors bound to extracellular matrix ligands is transmitted by the cytoskeleton to the cell nucleus inducing gene expression. Here, we used dendrimer-based...
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MDPI AG
2019-06-01
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Series: | Biomimetics |
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Online Access: | https://www.mdpi.com/2313-7673/4/2/43 |
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author | Ignasi Casanellas Anna Lagunas Yolanda Vida Ezequiel Pérez-Inestrosa José A. Andrades José Becerra Josep Samitier |
author_facet | Ignasi Casanellas Anna Lagunas Yolanda Vida Ezequiel Pérez-Inestrosa José A. Andrades José Becerra Josep Samitier |
author_sort | Ignasi Casanellas |
collection | DOAJ |
description | Extracellular matrix remodeling plays a pivotal role during mesenchyme patterning into different lineages. Tension exerted from cell membrane receptors bound to extracellular matrix ligands is transmitted by the cytoskeleton to the cell nucleus inducing gene expression. Here, we used dendrimer-based arginine−glycine−aspartic acid (RGD) uneven nanopatterns, which allow the control of local surface adhesiveness at the nanoscale, to unveil the adhesive requirements of mesenchymal tenogenic and osteogenic commitments. Cell response was found to depend on the tension resulting from cell−substrate interactions, which affects nuclear morphology and is regulated by focal adhesion size and distribution. |
first_indexed | 2024-12-10T17:35:12Z |
format | Article |
id | doaj.art-d5f7a699f3c7446c92f6cc886b198cff |
institution | Directory Open Access Journal |
issn | 2313-7673 |
language | English |
last_indexed | 2024-12-10T17:35:12Z |
publishDate | 2019-06-01 |
publisher | MDPI AG |
record_format | Article |
series | Biomimetics |
spelling | doaj.art-d5f7a699f3c7446c92f6cc886b198cff2022-12-22T01:39:34ZengMDPI AGBiomimetics2313-76732019-06-01424310.3390/biomimetics4020043biomimetics4020043Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell FateIgnasi Casanellas0Anna Lagunas1Yolanda Vida2Ezequiel Pérez-Inestrosa3José A. Andrades4José Becerra5Josep Samitier6Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainNetworking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, SpainDepartamento de Química Orgánica, Facultad de Ciencias, Universidad de Málaga-IBIMA, 29071 Málaga, SpainDepartamento de Química Orgánica, Facultad de Ciencias, Universidad de Málaga-IBIMA, 29071 Málaga, SpainDepartment of Cell Biology, Genetics and Physiology, Universidad de Málaga-IBIMA, 29071 Málaga, SpainDepartment of Cell Biology, Genetics and Physiology, Universidad de Málaga-IBIMA, 29071 Málaga, SpainInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainExtracellular matrix remodeling plays a pivotal role during mesenchyme patterning into different lineages. Tension exerted from cell membrane receptors bound to extracellular matrix ligands is transmitted by the cytoskeleton to the cell nucleus inducing gene expression. Here, we used dendrimer-based arginine−glycine−aspartic acid (RGD) uneven nanopatterns, which allow the control of local surface adhesiveness at the nanoscale, to unveil the adhesive requirements of mesenchymal tenogenic and osteogenic commitments. Cell response was found to depend on the tension resulting from cell−substrate interactions, which affects nuclear morphology and is regulated by focal adhesion size and distribution.https://www.mdpi.com/2313-7673/4/2/43arginine–glycine–aspartic acid (RGD)nanopatternmesenchymal stem cellstenogenesisosteogenesiscell nucleifocal adhesions |
spellingShingle | Ignasi Casanellas Anna Lagunas Yolanda Vida Ezequiel Pérez-Inestrosa José A. Andrades José Becerra Josep Samitier Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate Biomimetics arginine–glycine–aspartic acid (RGD) nanopattern mesenchymal stem cells tenogenesis osteogenesis cell nuclei focal adhesions |
title | Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate |
title_full | Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate |
title_fullStr | Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate |
title_full_unstemmed | Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate |
title_short | Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate |
title_sort | matrix nanopatterning regulates mesenchymal differentiation through focal adhesion size and distribution according to cell fate |
topic | arginine–glycine–aspartic acid (RGD) nanopattern mesenchymal stem cells tenogenesis osteogenesis cell nuclei focal adhesions |
url | https://www.mdpi.com/2313-7673/4/2/43 |
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