Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate

Extracellular matrix remodeling plays a pivotal role during mesenchyme patterning into different lineages. Tension exerted from cell membrane receptors bound to extracellular matrix ligands is transmitted by the cytoskeleton to the cell nucleus inducing gene expression. Here, we used dendrimer-based...

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Main Authors: Ignasi Casanellas, Anna Lagunas, Yolanda Vida, Ezequiel Pérez-Inestrosa, José A. Andrades, José Becerra, Josep Samitier
Format: Article
Language:English
Published: MDPI AG 2019-06-01
Series:Biomimetics
Subjects:
Online Access:https://www.mdpi.com/2313-7673/4/2/43
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author Ignasi Casanellas
Anna Lagunas
Yolanda Vida
Ezequiel Pérez-Inestrosa
José A. Andrades
José Becerra
Josep Samitier
author_facet Ignasi Casanellas
Anna Lagunas
Yolanda Vida
Ezequiel Pérez-Inestrosa
José A. Andrades
José Becerra
Josep Samitier
author_sort Ignasi Casanellas
collection DOAJ
description Extracellular matrix remodeling plays a pivotal role during mesenchyme patterning into different lineages. Tension exerted from cell membrane receptors bound to extracellular matrix ligands is transmitted by the cytoskeleton to the cell nucleus inducing gene expression. Here, we used dendrimer-based arginine−glycine−aspartic acid (RGD) uneven nanopatterns, which allow the control of local surface adhesiveness at the nanoscale, to unveil the adhesive requirements of mesenchymal tenogenic and osteogenic commitments. Cell response was found to depend on the tension resulting from cell−substrate interactions, which affects nuclear morphology and is regulated by focal adhesion size and distribution.
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spelling doaj.art-d5f7a699f3c7446c92f6cc886b198cff2022-12-22T01:39:34ZengMDPI AGBiomimetics2313-76732019-06-01424310.3390/biomimetics4020043biomimetics4020043Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell FateIgnasi Casanellas0Anna Lagunas1Yolanda Vida2Ezequiel Pérez-Inestrosa3José A. Andrades4José Becerra5Josep Samitier6Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainNetworking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, SpainDepartamento de Química Orgánica, Facultad de Ciencias, Universidad de Málaga-IBIMA, 29071 Málaga, SpainDepartamento de Química Orgánica, Facultad de Ciencias, Universidad de Málaga-IBIMA, 29071 Málaga, SpainDepartment of Cell Biology, Genetics and Physiology, Universidad de Málaga-IBIMA, 29071 Málaga, SpainDepartment of Cell Biology, Genetics and Physiology, Universidad de Málaga-IBIMA, 29071 Málaga, SpainInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainExtracellular matrix remodeling plays a pivotal role during mesenchyme patterning into different lineages. Tension exerted from cell membrane receptors bound to extracellular matrix ligands is transmitted by the cytoskeleton to the cell nucleus inducing gene expression. Here, we used dendrimer-based arginine−glycine−aspartic acid (RGD) uneven nanopatterns, which allow the control of local surface adhesiveness at the nanoscale, to unveil the adhesive requirements of mesenchymal tenogenic and osteogenic commitments. Cell response was found to depend on the tension resulting from cell−substrate interactions, which affects nuclear morphology and is regulated by focal adhesion size and distribution.https://www.mdpi.com/2313-7673/4/2/43arginine–glycine–aspartic acid (RGD)nanopatternmesenchymal stem cellstenogenesisosteogenesiscell nucleifocal adhesions
spellingShingle Ignasi Casanellas
Anna Lagunas
Yolanda Vida
Ezequiel Pérez-Inestrosa
José A. Andrades
José Becerra
Josep Samitier
Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate
Biomimetics
arginine–glycine–aspartic acid (RGD)
nanopattern
mesenchymal stem cells
tenogenesis
osteogenesis
cell nuclei
focal adhesions
title Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate
title_full Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate
title_fullStr Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate
title_full_unstemmed Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate
title_short Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate
title_sort matrix nanopatterning regulates mesenchymal differentiation through focal adhesion size and distribution according to cell fate
topic arginine–glycine–aspartic acid (RGD)
nanopattern
mesenchymal stem cells
tenogenesis
osteogenesis
cell nuclei
focal adhesions
url https://www.mdpi.com/2313-7673/4/2/43
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