Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite Platform
A simple and highly sensitive electrochemical DNA aptasensor with high affinity for endocrine disrupting 17β-estradiol, was developed. Poly(3,4-ethylenedioxylthiophene) (PEDOT) doped with gold nanoparticles (AuNPs) was electrochemically synthesized and employed for the immobilization of biotinylated...
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| Format: | Article |
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MDPI AG
2010-11-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/10/11/9872/ |
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| author | Emmanuel Iwuoha Priscilla Baker Omotayo Arotiba Tesfaye T. Waryo Stephen N. Mailu Rasaq A. Olowu |
| author_facet | Emmanuel Iwuoha Priscilla Baker Omotayo Arotiba Tesfaye T. Waryo Stephen N. Mailu Rasaq A. Olowu |
| author_sort | Emmanuel Iwuoha |
| collection | DOAJ |
| description | A simple and highly sensitive electrochemical DNA aptasensor with high affinity for endocrine disrupting 17β-estradiol, was developed. Poly(3,4-ethylenedioxylthiophene) (PEDOT) doped with gold nanoparticles (AuNPs) was electrochemically synthesized and employed for the immobilization of biotinylated aptamer towards the detection of the target. The diffusion coefficient of the nanocomposite was 6.50 × 10−7 cm2 s−1, which showed that the nanocomposite was highly conducting. Electrochemical impedance investigation also revealed the catalytic properties of the nanocomposite with an exchange current value of 2.16 × 10−4 A, compared to 2.14 × 10−5 A obtained for the bare electrode. Streptavidin was covalently attached to the platform using carbodiimide chemistry and the aptamer immobilized via streptavidin—biotin interaction. The electrochemical signal generated from the aptamer–target molecule interaction was monitored electrochemically using cyclic voltammetry and square wave voltammetry in the presence of [Fe(CN)6]−3/−4 as a redox probe. The signal observed shows a current decrease due to interference of the bound 17β-estradiol. The current drop was proportional to the concentration of 17β-estradiol. The PEDOT/AuNP platform exhibited high electroactivity, with increased peak current. The platform was found suitable for the immobilization of the DNAaptamer. The aptasensor was able to distinguish 17β-estradiol from structurally similar endocrine disrupting chemicals denoting its specificity to 17β-estradiol. The detectable concentration range of the 17β-estradiol was 0.1 nM–100 nM, with a detection limit of 0.02 nM. |
| first_indexed | 2024-04-14T01:25:49Z |
| format | Article |
| id | doaj.art-d5f999a92f9f4c858bf0eab91f1175d6 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T01:25:49Z |
| publishDate | 2010-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-d5f999a92f9f4c858bf0eab91f1175d62022-12-22T02:20:27ZengMDPI AGSensors1424-82202010-11-0110119872989010.3390/s101109872Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite PlatformEmmanuel IwuohaPriscilla BakerOmotayo ArotibaTesfaye T. WaryoStephen N. MailuRasaq A. OlowuA simple and highly sensitive electrochemical DNA aptasensor with high affinity for endocrine disrupting 17β-estradiol, was developed. Poly(3,4-ethylenedioxylthiophene) (PEDOT) doped with gold nanoparticles (AuNPs) was electrochemically synthesized and employed for the immobilization of biotinylated aptamer towards the detection of the target. The diffusion coefficient of the nanocomposite was 6.50 × 10−7 cm2 s−1, which showed that the nanocomposite was highly conducting. Electrochemical impedance investigation also revealed the catalytic properties of the nanocomposite with an exchange current value of 2.16 × 10−4 A, compared to 2.14 × 10−5 A obtained for the bare electrode. Streptavidin was covalently attached to the platform using carbodiimide chemistry and the aptamer immobilized via streptavidin—biotin interaction. The electrochemical signal generated from the aptamer–target molecule interaction was monitored electrochemically using cyclic voltammetry and square wave voltammetry in the presence of [Fe(CN)6]−3/−4 as a redox probe. The signal observed shows a current decrease due to interference of the bound 17β-estradiol. The current drop was proportional to the concentration of 17β-estradiol. The PEDOT/AuNP platform exhibited high electroactivity, with increased peak current. The platform was found suitable for the immobilization of the DNAaptamer. The aptasensor was able to distinguish 17β-estradiol from structurally similar endocrine disrupting chemicals denoting its specificity to 17β-estradiol. The detectable concentration range of the 17β-estradiol was 0.1 nM–100 nM, with a detection limit of 0.02 nM.http://www.mdpi.com/1424-8220/10/11/9872/poly(3,4-ethylenedioxythiophene) (PEDOT)aptamermodified electrodeavidin/biotinsquare wave voltammetry |
| spellingShingle | Emmanuel Iwuoha Priscilla Baker Omotayo Arotiba Tesfaye T. Waryo Stephen N. Mailu Rasaq A. Olowu Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite Platform Sensors poly(3,4-ethylenedioxythiophene) (PEDOT) aptamer modified electrode avidin/biotin square wave voltammetry |
| title | Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite Platform |
| title_full | Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite Platform |
| title_fullStr | Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite Platform |
| title_full_unstemmed | Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite Platform |
| title_short | Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite Platform |
| title_sort | electrochemical aptasensor for endocrine disrupting 17β estradiol based on a poly 3 4 ethylenedioxylthiopene gold nanocomposite platform |
| topic | poly(3,4-ethylenedioxythiophene) (PEDOT) aptamer modified electrode avidin/biotin square wave voltammetry |
| url | http://www.mdpi.com/1424-8220/10/11/9872/ |
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