Mechanisms of regulation of motility of the gastrointestinal tract and the hepatobiliary system under the chronic action of nanocolloids
Abstract Modern cutting edge technologies of chemical synthesis enable the production of unique nanostructures with excess energy and high reactivity. Uncontrolled use of such materials in the food industry and pharmacology entail a risk for the development of a nanotoxicity crisis. Using the method...
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-30958-5 |
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| author | Olga V. Tsymbalyuk Tamara L. Davydovska Anna M. Naumenko Ivan S. Voiteshenko Stanislav P. Veselsky Alex Y. Nyporko Anastasiia Y. Pidhaietska Mariya S. Kozolup Valeriy A. Skryshevsky |
| author_facet | Olga V. Tsymbalyuk Tamara L. Davydovska Anna M. Naumenko Ivan S. Voiteshenko Stanislav P. Veselsky Alex Y. Nyporko Anastasiia Y. Pidhaietska Mariya S. Kozolup Valeriy A. Skryshevsky |
| author_sort | Olga V. Tsymbalyuk |
| collection | DOAJ |
| description | Abstract Modern cutting edge technologies of chemical synthesis enable the production of unique nanostructures with excess energy and high reactivity. Uncontrolled use of such materials in the food industry and pharmacology entail a risk for the development of a nanotoxicity crisis. Using the methods of tensometry, mechanokinetic analysis, biochemical methods, and bioinformatics, the current study showed that chronic (for six months) intragastrical burdening of rats with aqueous nanocolloids (AN) ZnO and TiO2 caused violations of the pacemaker-dependent mechanisms of regulation of spontaneous and neurotransmitter-induced contractions of the gastrointestinal tract (GIT) smooth muscles (SMs), and transformed the contraction efficiency indices (AU, in Alexandria units). Under the same conditions, the fundamental principle of distribution of physiologically relevant differences in the numeric values of the mechanokinetic parameters of spontaneous SM contractions between different parts of GIT is violated, which can potentially cause its pathological changes. Using molecular docking, typical bonds in the interfaces of the interaction of these nanomaterials with myosin II, a component of the contractile apparatus of smooth muscle cells (SMC) were investigated. In this connection, the study addressed the question of possible competitive relations between ZnO and TiO2 nanoparticles and actin molecules for binding sites on the myosin II actin-interaction interface. In addition, using biochemical methods, it was shown that chronic long-term exposure to nanocolloids causes changes in the primary active ion transport systems of cell plasma membranes, the activity of marker liver enzymes and disrupts the blood plasma lipid profile, which indicates the hepatotoxic effect of these nanocolloids. |
| first_indexed | 2024-04-09T22:57:45Z |
| format | Article |
| id | doaj.art-d5fba4204e734916aca744ac6b4b5a74 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:57:45Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d5fba4204e734916aca744ac6b4b5a742023-03-22T11:13:02ZengNature PortfolioScientific Reports2045-23222023-03-0113111410.1038/s41598-023-30958-5Mechanisms of regulation of motility of the gastrointestinal tract and the hepatobiliary system under the chronic action of nanocolloidsOlga V. Tsymbalyuk0Tamara L. Davydovska1Anna M. Naumenko2Ivan S. Voiteshenko3Stanislav P. Veselsky4Alex Y. Nyporko5Anastasiia Y. Pidhaietska6Mariya S. Kozolup7Valeriy A. Skryshevsky8Institute of High Technologies, Taras Shevchenko National University of KyivInstitute of High Technologies, Taras Shevchenko National University of KyivInstitute of High Technologies, Taras Shevchenko National University of KyivInstitute of High Technologies, Taras Shevchenko National University of KyivInstitute of High Technologies, Taras Shevchenko National University of KyivInstitute of High Technologies, Taras Shevchenko National University of KyivInstitute of High Technologies, Taras Shevchenko National University of KyivDepartment of Foreign Languages for Sciences, Ivan Franko National University of LvivInstitute of High Technologies, Taras Shevchenko National University of KyivAbstract Modern cutting edge technologies of chemical synthesis enable the production of unique nanostructures with excess energy and high reactivity. Uncontrolled use of such materials in the food industry and pharmacology entail a risk for the development of a nanotoxicity crisis. Using the methods of tensometry, mechanokinetic analysis, biochemical methods, and bioinformatics, the current study showed that chronic (for six months) intragastrical burdening of rats with aqueous nanocolloids (AN) ZnO and TiO2 caused violations of the pacemaker-dependent mechanisms of regulation of spontaneous and neurotransmitter-induced contractions of the gastrointestinal tract (GIT) smooth muscles (SMs), and transformed the contraction efficiency indices (AU, in Alexandria units). Under the same conditions, the fundamental principle of distribution of physiologically relevant differences in the numeric values of the mechanokinetic parameters of spontaneous SM contractions between different parts of GIT is violated, which can potentially cause its pathological changes. Using molecular docking, typical bonds in the interfaces of the interaction of these nanomaterials with myosin II, a component of the contractile apparatus of smooth muscle cells (SMC) were investigated. In this connection, the study addressed the question of possible competitive relations between ZnO and TiO2 nanoparticles and actin molecules for binding sites on the myosin II actin-interaction interface. In addition, using biochemical methods, it was shown that chronic long-term exposure to nanocolloids causes changes in the primary active ion transport systems of cell plasma membranes, the activity of marker liver enzymes and disrupts the blood plasma lipid profile, which indicates the hepatotoxic effect of these nanocolloids.https://doi.org/10.1038/s41598-023-30958-5 |
| spellingShingle | Olga V. Tsymbalyuk Tamara L. Davydovska Anna M. Naumenko Ivan S. Voiteshenko Stanislav P. Veselsky Alex Y. Nyporko Anastasiia Y. Pidhaietska Mariya S. Kozolup Valeriy A. Skryshevsky Mechanisms of regulation of motility of the gastrointestinal tract and the hepatobiliary system under the chronic action of nanocolloids Scientific Reports |
| title | Mechanisms of regulation of motility of the gastrointestinal tract and the hepatobiliary system under the chronic action of nanocolloids |
| title_full | Mechanisms of regulation of motility of the gastrointestinal tract and the hepatobiliary system under the chronic action of nanocolloids |
| title_fullStr | Mechanisms of regulation of motility of the gastrointestinal tract and the hepatobiliary system under the chronic action of nanocolloids |
| title_full_unstemmed | Mechanisms of regulation of motility of the gastrointestinal tract and the hepatobiliary system under the chronic action of nanocolloids |
| title_short | Mechanisms of regulation of motility of the gastrointestinal tract and the hepatobiliary system under the chronic action of nanocolloids |
| title_sort | mechanisms of regulation of motility of the gastrointestinal tract and the hepatobiliary system under the chronic action of nanocolloids |
| url | https://doi.org/10.1038/s41598-023-30958-5 |
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