Mitochondrial control region diversity in Polish sheep breeds
The aim of the study was to determine the genetic variability of the Polish sheep breeds Świniarka, Wrzosówka, Pomorska, and Wielkopolska based on mitochondrial control region polymorphism. A comprehensive phylogenetic analysis and information about the genetic origin of the breeds were also obtaine...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2016-05-01
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| Series: | Archives Animal Breeding |
| Online Access: | http://www.arch-anim-breed.net/59/227/2016/aab-59-227-2016.pdf |
| Summary: | The aim of the study was to determine the genetic variability of the Polish sheep breeds Świniarka, Wrzosówka, Pomorska, and
Wielkopolska based on mitochondrial control region polymorphism. A comprehensive phylogenetic analysis and information about
the genetic origin of the breeds were also obtained. The genetic variability of the breeds studied
has been assessed based on the number of haplotypes, haplotype diversity,
nucleotide diversity, the average number of nucleotide differences, the number
of mutations, and phylogenetically informative sites. Sequence divergence
between identified haplogroup A (HA) and haplogroup B (HB) was also
calculated. Moreover, a neighbour-joining (NJ) haplotype tree was
constructed based on Kimura's two-parameter genetic distance calculation.
Finally, the history of the population was investigated by mismatch distribution
and Fu's <i>F</i> statistics. The 559 bp long mitochondrial DNA (mtDNA) control region (CR) sequences of 143 sheep were
analysed. The 65 haplotypes were defined by 45 parsimony informative sites.
Among the four Polish breeds, Wrzosówka had the highest while Świniarka
the lowest values of haplotype (<i>H</i><sub>d</sub>) and sequence diversity (<i>π</i>)
(<i>H</i><sub>d</sub> = 0.9735 and <i>π</i> = 0.0040 for Wrzosówka;
<i>H</i><sub>d</sub> = 0.8975 and <i>π</i> = 0.0030 for Świniarka). Five haplotypes
were shared between breeds, whereas the remaining 60 were unique. The NJ
phylogenetic tree has revealed that 61 haplotypes of all analysed breeds
clustered into clade B while the remaining 4 haplotypes representing all but
the Świniarka breed pooled together with clade A. None of the other reported
mitochondrial haplogroups were identified. The haplotypes representing HB
formed a star-like network with the single central haplotype, which in
association with extensive haplotype sharing reveals a weak structure of
Polish breeds and the existence of gene flow between the breeds studied. |
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| ISSN: | 0003-9438 2363-9822 |