Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system
The tissue inhibitors of metalloproteinases 3 (TIMP3) play an essential role in the tumorigenesis of human pancreatic endocrine tumors and Sorsby fundus dystrophy. To further investigate the significance of TIMP3 in disease, we used CRISPR/Cas9 to create a TIMP3 knock out human embryonic stem cell l...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350612300020X |
| _version_ | 1797897328778018816 |
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| author | Tong Yi Jingjing Liu Siyao Zhang Yihua He Jiancheng Han |
| author_facet | Tong Yi Jingjing Liu Siyao Zhang Yihua He Jiancheng Han |
| author_sort | Tong Yi |
| collection | DOAJ |
| description | The tissue inhibitors of metalloproteinases 3 (TIMP3) play an essential role in the tumorigenesis of human pancreatic endocrine tumors and Sorsby fundus dystrophy. To further investigate the significance of TIMP3 in disease, we used CRISPR/Cas9 to create a TIMP3 knock out human embryonic stem cell line (WAe009-A-89) that can differentiate into any desired cell type. Our results show that the WAe009-A-89 cell line retains the typical colony form and normal karyotype of stem cells. The cells strongly expressed pluripotency markers and could differentiate into tissues of all three germ layers in vivo. This cell line allowed exploring the role of the TIMP3 gene in related diseases. |
| first_indexed | 2024-04-10T07:55:46Z |
| format | Article |
| id | doaj.art-d5fd3f92bca54b0c889727d746458939 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-10T07:55:46Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-d5fd3f92bca54b0c889727d7464589392023-02-23T04:30:55ZengElsevierStem Cell Research1873-50612023-03-0167103034Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 systemTong Yi0Jingjing Liu1Siyao Zhang2Yihua He3Jiancheng Han4Department of Ultrasound, Beijing Anzhen Hospital, Capital Medical University, Beijing Key Laboratory of Maternal-Fetus Medicine in Fetal Heart Disease (BZ0308), Beijing, People's Republic of ChinaDepartment of Ultrasound, Beijing Anzhen Hospital, Capital Medical University, Beijing Key Laboratory of Maternal-Fetus Medicine in Fetal Heart Disease (BZ0308), Beijing, People's Republic of ChinaDepartment of Ultrasound, Beijing Anzhen Hospital, Capital Medical University, Beijing Key Laboratory of Maternal-Fetus Medicine in Fetal Heart Disease (BZ0308), Beijing, People's Republic of ChinaCorresponding authors at: Department of Ultrasound, Beijing Anzhen Hospital, Beijing Key Laboratory of Maternal-Fetus Medicine in Fetal Heart Disease (BZ0308), No.2, Anzhen Road, Chaoyang District, Beijing 100029, People's Republic of China.; Department of Ultrasound, Beijing Anzhen Hospital, Capital Medical University, Beijing Key Laboratory of Maternal-Fetus Medicine in Fetal Heart Disease (BZ0308), Beijing, People's Republic of ChinaCorresponding authors at: Department of Ultrasound, Beijing Anzhen Hospital, Beijing Key Laboratory of Maternal-Fetus Medicine in Fetal Heart Disease (BZ0308), No.2, Anzhen Road, Chaoyang District, Beijing 100029, People's Republic of China.; Department of Ultrasound, Beijing Anzhen Hospital, Capital Medical University, Beijing Key Laboratory of Maternal-Fetus Medicine in Fetal Heart Disease (BZ0308), Beijing, People's Republic of ChinaThe tissue inhibitors of metalloproteinases 3 (TIMP3) play an essential role in the tumorigenesis of human pancreatic endocrine tumors and Sorsby fundus dystrophy. To further investigate the significance of TIMP3 in disease, we used CRISPR/Cas9 to create a TIMP3 knock out human embryonic stem cell line (WAe009-A-89) that can differentiate into any desired cell type. Our results show that the WAe009-A-89 cell line retains the typical colony form and normal karyotype of stem cells. The cells strongly expressed pluripotency markers and could differentiate into tissues of all three germ layers in vivo. This cell line allowed exploring the role of the TIMP3 gene in related diseases.http://www.sciencedirect.com/science/article/pii/S187350612300020X |
| spellingShingle | Tong Yi Jingjing Liu Siyao Zhang Yihua He Jiancheng Han Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system Stem Cell Research |
| title | Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system |
| title_full | Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system |
| title_fullStr | Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system |
| title_full_unstemmed | Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system |
| title_short | Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system |
| title_sort | generation of a timp3 knockout stem cell line via crispr cas9 system |
| url | http://www.sciencedirect.com/science/article/pii/S187350612300020X |
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