RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from <i>Lactiplantibacillus plantarum</i>
Bacteria generally release extracellular membrane vesicles (MVs), which are nanoparticles that play important roles in bacterial–bacterial and bacterial–host communication. As probiotics, lactic acid bacteria provide diverse health benefits to their hosts. In this study, we found that the Gram-posit...
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MDPI AG
2024-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/6/967 |
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| author | Shino Yamasaki-Yashiki Fumie Kawashima Azusa Saika Ryota Hosomi Jun Kunisawa Yoshio Katakura |
| author_facet | Shino Yamasaki-Yashiki Fumie Kawashima Azusa Saika Ryota Hosomi Jun Kunisawa Yoshio Katakura |
| author_sort | Shino Yamasaki-Yashiki |
| collection | DOAJ |
| description | Bacteria generally release extracellular membrane vesicles (MVs), which are nanoparticles that play important roles in bacterial–bacterial and bacterial–host communication. As probiotics, lactic acid bacteria provide diverse health benefits to their hosts. In this study, we found that the Gram-positive lactic acid bacteria <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> NBRC 15891 produce high amounts of MVs (<i>Lp</i>MVs), and that <i>Lp</i>MVs inhibit interleukin (IL)-8 production induced by lipopolysaccharide in intestinal epithelial HT29 cells. Heat- or UV-killed bacterial cells did not exhibit anti-inflammatory effects, and there was no uptake of these bacterial cells; contrarily, <i>Lp</i>MVs were taken up into the cytoplasm of HT29 cells. Small RNAs extracted from <i>Lp</i>MVs also suppressed IL-8 production in HT29 cells, suggesting that RNAs in the cytoplasm of bacterial cells are encapsulated in the MVs and released from the cells, which may be delivered to HT29 cells to exert their anti-inflammatory effects. In addition, administration of <i>Lp</i>MVs to mice with dextran sodium sulfate-induced colitis alleviated colitis-induced weight loss and colon length shortening, indicating that <i>Lp</i>MV intake is likely to be effective in preventing or ameliorating colitis. |
| first_indexed | 2024-04-24T18:17:23Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-24T18:17:23Z |
| publishDate | 2024-03-01 |
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| series | Foods |
| spelling | doaj.art-d5ffbc36d2a442d0859135a3a24238552024-03-27T13:41:20ZengMDPI AGFoods2304-81582024-03-0113696710.3390/foods13060967RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from <i>Lactiplantibacillus plantarum</i>Shino Yamasaki-Yashiki0Fumie Kawashima1Azusa Saika2Ryota Hosomi3Jun Kunisawa4Yoshio Katakura5Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, JapanDepartment of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, JapanLaboratory of Vaccine Materials, Laboratory of Gut Environmental System, Microbial Research Center for Health and Medicine, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Ibaraki, Osaka 567-0085, JapanDepartment of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, JapanLaboratory of Vaccine Materials, Laboratory of Gut Environmental System, Microbial Research Center for Health and Medicine, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Ibaraki, Osaka 567-0085, JapanDepartment of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, JapanBacteria generally release extracellular membrane vesicles (MVs), which are nanoparticles that play important roles in bacterial–bacterial and bacterial–host communication. As probiotics, lactic acid bacteria provide diverse health benefits to their hosts. In this study, we found that the Gram-positive lactic acid bacteria <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> NBRC 15891 produce high amounts of MVs (<i>Lp</i>MVs), and that <i>Lp</i>MVs inhibit interleukin (IL)-8 production induced by lipopolysaccharide in intestinal epithelial HT29 cells. Heat- or UV-killed bacterial cells did not exhibit anti-inflammatory effects, and there was no uptake of these bacterial cells; contrarily, <i>Lp</i>MVs were taken up into the cytoplasm of HT29 cells. Small RNAs extracted from <i>Lp</i>MVs also suppressed IL-8 production in HT29 cells, suggesting that RNAs in the cytoplasm of bacterial cells are encapsulated in the MVs and released from the cells, which may be delivered to HT29 cells to exert their anti-inflammatory effects. In addition, administration of <i>Lp</i>MVs to mice with dextran sodium sulfate-induced colitis alleviated colitis-induced weight loss and colon length shortening, indicating that <i>Lp</i>MV intake is likely to be effective in preventing or ameliorating colitis.https://www.mdpi.com/2304-8158/13/6/967membrane vesiclelactic acid bacteriaRNAanti-inflammatory effectgut |
| spellingShingle | Shino Yamasaki-Yashiki Fumie Kawashima Azusa Saika Ryota Hosomi Jun Kunisawa Yoshio Katakura RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from <i>Lactiplantibacillus plantarum</i> Foods membrane vesicle lactic acid bacteria RNA anti-inflammatory effect gut |
| title | RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from <i>Lactiplantibacillus plantarum</i> |
| title_full | RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from <i>Lactiplantibacillus plantarum</i> |
| title_fullStr | RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from <i>Lactiplantibacillus plantarum</i> |
| title_full_unstemmed | RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from <i>Lactiplantibacillus plantarum</i> |
| title_short | RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from <i>Lactiplantibacillus plantarum</i> |
| title_sort | rna based anti inflammatory effects of membrane vesicles derived from i lactiplantibacillus plantarum i |
| topic | membrane vesicle lactic acid bacteria RNA anti-inflammatory effect gut |
| url | https://www.mdpi.com/2304-8158/13/6/967 |
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