Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines
Abstract Four endemic human coronaviruses (HCoV), HCoV-229E, HCoV-NL63, HCoV-HKU1, and HCoV-OC43, are closely related to SARS-CoV-2. These coronaviruses are known to infect humans living in temperate areas, including children under 5 years old; however, the seroprevalence of four HCoVs among childre...
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| Format: | Article |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29072-3 |
| _version_ | 1811166029527646208 |
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| author | Yusuke Sayama Michiko Okamoto Mayuko Saito Mariko Saito-Obata Raita Tamaki Christine Dahlia Joboco Socorro Lupisan Hitoshi Oshitani |
| author_facet | Yusuke Sayama Michiko Okamoto Mayuko Saito Mariko Saito-Obata Raita Tamaki Christine Dahlia Joboco Socorro Lupisan Hitoshi Oshitani |
| author_sort | Yusuke Sayama |
| collection | DOAJ |
| description | Abstract Four endemic human coronaviruses (HCoV), HCoV-229E, HCoV-NL63, HCoV-HKU1, and HCoV-OC43, are closely related to SARS-CoV-2. These coronaviruses are known to infect humans living in temperate areas, including children under 5 years old; however, the seroprevalence of four HCoVs among children in tropical areas, including the Philippines, remains unclear. This study aimed to assess the prevalence of antibodies against four HCoVs and to determine the reactivity and neutralization of these antibodies against SARS-CoV-2 among children in the Philippines. A total of 315 serum samples collected from 2015 to 2018, before the emergence of SARS-CoV-2, in Biliran island, Philippines, were tested for the presence of antibodies against four HCoVs and SARS-CoV-2 using recombinant spike ectodomain proteins by IgG-enzyme-linked immunosorbent assay (ELISA). Reactivity to and neutralization of SARS-CoV-2 were also investigated. The seroprevalence of the four HCoVs was 63.8% for HCoV-229E, 71.4% for HCoV-NL63, 76.5% for HCoV-HKU1, and 83.5% for HCoV-OC43 by ELISA. Age group analysis indicated that seropositivity to all HCoVs reached 80% by 2–3 years of age. While 69/315 (21.9%) of the samples showed reactive to SARS-CoV-2, almost no neutralization against SARS-CoV-2 was detected using neutralization assay. Reactivity of antibodies against SARS-CoV-2 spike protein obtained by ELISA may not correlate with neutralization capability. |
| first_indexed | 2024-04-10T15:45:46Z |
| format | Article |
| id | doaj.art-d601216707f746c2bc4740fd2577e6a3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T15:45:46Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d601216707f746c2bc4740fd2577e6a32023-02-12T12:08:39ZengNature PortfolioScientific Reports2045-23222023-02-011311810.1038/s41598-023-29072-3Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the PhilippinesYusuke Sayama0Michiko Okamoto1Mayuko Saito2Mariko Saito-Obata3Raita Tamaki4Christine Dahlia Joboco5Socorro Lupisan6Hitoshi Oshitani7Department of Virology, Tohoku University Graduate School of MedicineDepartment of Virology, Tohoku University Graduate School of MedicineDepartment of Virology, Tohoku University Graduate School of MedicineDepartment of Virology, Tohoku University Graduate School of MedicineInstitute of Tropical Medicine, Nagasaki UniversityBiliran Provincial HospitalResearch Institute for Tropical MedicineDepartment of Virology, Tohoku University Graduate School of MedicineAbstract Four endemic human coronaviruses (HCoV), HCoV-229E, HCoV-NL63, HCoV-HKU1, and HCoV-OC43, are closely related to SARS-CoV-2. These coronaviruses are known to infect humans living in temperate areas, including children under 5 years old; however, the seroprevalence of four HCoVs among children in tropical areas, including the Philippines, remains unclear. This study aimed to assess the prevalence of antibodies against four HCoVs and to determine the reactivity and neutralization of these antibodies against SARS-CoV-2 among children in the Philippines. A total of 315 serum samples collected from 2015 to 2018, before the emergence of SARS-CoV-2, in Biliran island, Philippines, were tested for the presence of antibodies against four HCoVs and SARS-CoV-2 using recombinant spike ectodomain proteins by IgG-enzyme-linked immunosorbent assay (ELISA). Reactivity to and neutralization of SARS-CoV-2 were also investigated. The seroprevalence of the four HCoVs was 63.8% for HCoV-229E, 71.4% for HCoV-NL63, 76.5% for HCoV-HKU1, and 83.5% for HCoV-OC43 by ELISA. Age group analysis indicated that seropositivity to all HCoVs reached 80% by 2–3 years of age. While 69/315 (21.9%) of the samples showed reactive to SARS-CoV-2, almost no neutralization against SARS-CoV-2 was detected using neutralization assay. Reactivity of antibodies against SARS-CoV-2 spike protein obtained by ELISA may not correlate with neutralization capability.https://doi.org/10.1038/s41598-023-29072-3 |
| spellingShingle | Yusuke Sayama Michiko Okamoto Mayuko Saito Mariko Saito-Obata Raita Tamaki Christine Dahlia Joboco Socorro Lupisan Hitoshi Oshitani Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines Scientific Reports |
| title | Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines |
| title_full | Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines |
| title_fullStr | Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines |
| title_full_unstemmed | Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines |
| title_short | Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines |
| title_sort | seroprevalence of four endemic human coronaviruses and reactivity and neutralization capability against sars cov 2 among children in the philippines |
| url | https://doi.org/10.1038/s41598-023-29072-3 |
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