Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cells
Transforming growth factor-β1 (TGF-β1) plays an important role in the fibrogenic process in the liver. The aim of the present study was to explore the action of TGF-β1 on fibronectin expression in rat hepatic stem-like cells and the underlying mechanisms. The level of fibr...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2010-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100006 |
| _version_ | 1818335695614246912 |
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| author | W. Cui H.B. Jin Z.W. Li |
| author_facet | W. Cui H.B. Jin Z.W. Li |
| author_sort | W. Cui |
| collection | DOAJ |
| description | Transforming growth factor-β1 (TGF-β1) plays an important role in the fibrogenic process in the liver. The aim of the present study was to explore the action of TGF-β1 on fibronectin expression in rat hepatic stem-like cells and the underlying mechanisms. The level of fibronectin expression was determined in hepatic stem-like cells (WB cells) before and after TGF-β1 stimulation by RT-PCR and Western blot methods. Using immunogold transmission electron microscopy and the Western blot method, we observed the result of the expression and the distribution of cAMP, phosphorylated Smad3 and Smad7 before and after TGF-β1 treatment. The levels of fibronectin expression in both mRNA and protein increased 4- to 5-fold after TGF-β1 stimulation, reaching an optimum level after 8 h and then gradually falling back. Similarly, TGF-β1 stimulation resulted in an increase of cAMP in WB cells, peaking at 8 h. After treatment with TGF-β1 for 24 h, the expression of cAMP gradually decreased. In addition, we found that TGF-β1 treatment also contributed to the increased expression and to changes in cellular distribution of phosphorylated Smad3 (translocation from the cytoplasm to the nucleus) and Smad7 (translocation from the nucleus to the cytoplasm) in WB cells. The present study demonstrates that TGF-β is involved in the fibrogenic process in hepatic stem cells through up-regulation of fibronectin expression, and the mechanisms underlying this process may be associated with the activation of cAMP and Smad pathways. |
| first_indexed | 2024-12-13T14:27:31Z |
| format | Article |
| id | doaj.art-d60264af46fd497d9818ab660e1c8a43 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-13T14:27:31Z |
| publishDate | 2010-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-d60264af46fd497d9818ab660e1c8a432022-12-21T23:41:54ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2010-01-014313642Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cellsW. CuiH.B. JinZ.W. LiTransforming growth factor-β1 (TGF-β1) plays an important role in the fibrogenic process in the liver. The aim of the present study was to explore the action of TGF-β1 on fibronectin expression in rat hepatic stem-like cells and the underlying mechanisms. The level of fibronectin expression was determined in hepatic stem-like cells (WB cells) before and after TGF-β1 stimulation by RT-PCR and Western blot methods. Using immunogold transmission electron microscopy and the Western blot method, we observed the result of the expression and the distribution of cAMP, phosphorylated Smad3 and Smad7 before and after TGF-β1 treatment. The levels of fibronectin expression in both mRNA and protein increased 4- to 5-fold after TGF-β1 stimulation, reaching an optimum level after 8 h and then gradually falling back. Similarly, TGF-β1 stimulation resulted in an increase of cAMP in WB cells, peaking at 8 h. After treatment with TGF-β1 for 24 h, the expression of cAMP gradually decreased. In addition, we found that TGF-β1 treatment also contributed to the increased expression and to changes in cellular distribution of phosphorylated Smad3 (translocation from the cytoplasm to the nucleus) and Smad7 (translocation from the nucleus to the cytoplasm) in WB cells. The present study demonstrates that TGF-β is involved in the fibrogenic process in hepatic stem cells through up-regulation of fibronectin expression, and the mechanisms underlying this process may be associated with the activation of cAMP and Smad pathways.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100006TGF-βFibronectinFibrogenesisHepatic stem cellcAMPSmad |
| spellingShingle | W. Cui H.B. Jin Z.W. Li Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cells Brazilian Journal of Medical and Biological Research TGF-β Fibronectin Fibrogenesis Hepatic stem cell cAMP Smad |
| title | Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cells |
| title_full | Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cells |
| title_fullStr | Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cells |
| title_full_unstemmed | Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cells |
| title_short | Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cells |
| title_sort | mechanism of the transforming growth factor 946 induction of fibronectin expression in hepatic stem like cells |
| topic | TGF-β Fibronectin Fibrogenesis Hepatic stem cell cAMP Smad |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100006 |
| work_keys_str_mv | AT wcui mechanismofthetransforminggrowthfactor946inductionoffibronectinexpressioninhepaticstemlikecells AT hbjin mechanismofthetransforminggrowthfactor946inductionoffibronectinexpressioninhepaticstemlikecells AT zwli mechanismofthetransforminggrowthfactor946inductionoffibronectinexpressioninhepaticstemlikecells |