Common Raman Spectral Markers among Different Tissues for Cancer Detection
Introduction Raman spectroscopy is a vibrational spectroscopic technique, based on inelastic scattering of monochromatic light. This technique can provide valuable information about biomolecular changes, associated with neoplastic transformation. The purpose of this study was to find Raman spectral...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2014-11-01
|
| Series: | Iranian Journal of Medical Physics |
| Subjects: | |
| Online Access: | http://ijmp.mums.ac.ir/article_3567_2bb3744f4223f66a0402809c859bbd05.pdf |
| _version_ | 1811274723690020864 |
|---|---|
| author | Zohreh Dehghani-Bidgoli Mohammad Hosein Miran Baygi Ehsanollah Kabir Rasoul Malekfar |
| author_facet | Zohreh Dehghani-Bidgoli Mohammad Hosein Miran Baygi Ehsanollah Kabir Rasoul Malekfar |
| author_sort | Zohreh Dehghani-Bidgoli |
| collection | DOAJ |
| description | Introduction Raman spectroscopy is a vibrational spectroscopic technique, based on inelastic scattering of monochromatic light. This technique can provide valuable information about biomolecular changes, associated with neoplastic transformation. The purpose of this study was to find Raman spectral markers for distinguishing normal samples from cancerous ones in different tissues. Materials and Methods Ten tissue samples from the breast, colon, pancreas, and thyroid were collected. A Raman system was used for Raman spectroscopic measurement of tissues at 532 nm laser excitation. Five to six Raman spectra were acquired from each sample (a total of 52 spectra). Raman spectra were investigated in important bands associated with Amid1, CH2 (scissoring), Amid3, d(NH), n(C-C), and das (CH3) in both normal and cancerous groups. In addition, common spectral markers, which discriminated between normal and cancerous samples in the above tissues, were investigated. Results Common spectral markers among different tissues included intensities of Amid3 and CH2 (scissoring) and intensity ratios of I(Amid1)/I(CH2), I(n(C-C))/I(CH2), and I(d(NH))/I(CH2). This study showed that Amid1-, n(C-C)-, and d(NH)-to-CH2 intensity ratios can discriminate between normal and cancerous samples, with an accuracy of 84.6%, 82.7%, and 82.7% in all studied tissues, respectively. Conclusion This study demonstrates the presence of common spectral markers, associated with neoplastic changes, among different tissues. |
| first_indexed | 2024-04-12T23:25:18Z |
| format | Article |
| id | doaj.art-d602db5af8de4f39a1b16bd47fa5a4db |
| institution | Directory Open Access Journal |
| issn | 2345-3672 2345-3672 |
| language | English |
| last_indexed | 2024-04-12T23:25:18Z |
| publishDate | 2014-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Physics |
| spelling | doaj.art-d602db5af8de4f39a1b16bd47fa5a4db2022-12-22T03:12:26ZengMashhad University of Medical SciencesIranian Journal of Medical Physics2345-36722345-36722014-11-0111430831510.22038/ijmp.2014.35673567Common Raman Spectral Markers among Different Tissues for Cancer DetectionZohreh Dehghani-Bidgoli0Mohammad Hosein Miran Baygi1Ehsanollah Kabir2Rasoul Malekfar3Department of Electrical and Computer Engineering, Kashan Branch, Islamic Azad University, Kashan, IranDepartment of Electrical and Computer Engineering, Tarbiat Modares University, Tehran, IranDepartment of Electrical and Computer Engineering, Tarbiat Modares University, Tehran, IranDepartment of Basic Sciences, Tarbiat Modares University, Tehran, IranIntroduction Raman spectroscopy is a vibrational spectroscopic technique, based on inelastic scattering of monochromatic light. This technique can provide valuable information about biomolecular changes, associated with neoplastic transformation. The purpose of this study was to find Raman spectral markers for distinguishing normal samples from cancerous ones in different tissues. Materials and Methods Ten tissue samples from the breast, colon, pancreas, and thyroid were collected. A Raman system was used for Raman spectroscopic measurement of tissues at 532 nm laser excitation. Five to six Raman spectra were acquired from each sample (a total of 52 spectra). Raman spectra were investigated in important bands associated with Amid1, CH2 (scissoring), Amid3, d(NH), n(C-C), and das (CH3) in both normal and cancerous groups. In addition, common spectral markers, which discriminated between normal and cancerous samples in the above tissues, were investigated. Results Common spectral markers among different tissues included intensities of Amid3 and CH2 (scissoring) and intensity ratios of I(Amid1)/I(CH2), I(n(C-C))/I(CH2), and I(d(NH))/I(CH2). This study showed that Amid1-, n(C-C)-, and d(NH)-to-CH2 intensity ratios can discriminate between normal and cancerous samples, with an accuracy of 84.6%, 82.7%, and 82.7% in all studied tissues, respectively. Conclusion This study demonstrates the presence of common spectral markers, associated with neoplastic changes, among different tissues.http://ijmp.mums.ac.ir/article_3567_2bb3744f4223f66a0402809c859bbd05.pdfBreast cancercancer detectionColon cancerpancreas cancerRaman Spectroscopythyroid cancer |
| spellingShingle | Zohreh Dehghani-Bidgoli Mohammad Hosein Miran Baygi Ehsanollah Kabir Rasoul Malekfar Common Raman Spectral Markers among Different Tissues for Cancer Detection Iranian Journal of Medical Physics Breast cancer cancer detection Colon cancer pancreas cancer Raman Spectroscopy thyroid cancer |
| title | Common Raman Spectral Markers among Different Tissues for Cancer Detection |
| title_full | Common Raman Spectral Markers among Different Tissues for Cancer Detection |
| title_fullStr | Common Raman Spectral Markers among Different Tissues for Cancer Detection |
| title_full_unstemmed | Common Raman Spectral Markers among Different Tissues for Cancer Detection |
| title_short | Common Raman Spectral Markers among Different Tissues for Cancer Detection |
| title_sort | common raman spectral markers among different tissues for cancer detection |
| topic | Breast cancer cancer detection Colon cancer pancreas cancer Raman Spectroscopy thyroid cancer |
| url | http://ijmp.mums.ac.ir/article_3567_2bb3744f4223f66a0402809c859bbd05.pdf |
| work_keys_str_mv | AT zohrehdehghanibidgoli commonramanspectralmarkersamongdifferenttissuesforcancerdetection AT mohammadhoseinmiranbaygi commonramanspectralmarkersamongdifferenttissuesforcancerdetection AT ehsanollahkabir commonramanspectralmarkersamongdifferenttissuesforcancerdetection AT rasoulmalekfar commonramanspectralmarkersamongdifferenttissuesforcancerdetection |