In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung
Increasing natural ingredient awareness and utilization has created an increased demand for sources of natural medicinal ingredients, including sources of compound used to treat malaria. Streptomyces is a genus of prokaryote well recognized for its production of antibiotics and other pharmaceuticall...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2021-06-01
|
| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/in-vitro-test-for-inhibition-of-plasmodium-falciparum-3d7-parasites-using-streptomyces-hygroscopicus-subsp-hygroscopicus-strain-i18-isolated-from-a-pineapple-farm-in-lampung/ |
| _version_ | 1830179037491757056 |
|---|---|
| author | Endah Setyaningrum Achmad Arifiyanto Nismah Nukmal Titik Nur Aeny Meishy Handerlin Putri Ulin Ni’mah Setiawati |
| author_facet | Endah Setyaningrum Achmad Arifiyanto Nismah Nukmal Titik Nur Aeny Meishy Handerlin Putri Ulin Ni’mah Setiawati |
| author_sort | Endah Setyaningrum |
| collection | DOAJ |
| description | Increasing natural ingredient awareness and utilization has created an increased demand for sources of natural medicinal ingredients, including sources of compound used to treat malaria. Streptomyces is a genus of prokaryote well recognized for its production of antibiotics and other pharmaceutically useful compound. This study aimed to assess the ability of unpurified fermentation metabolites to inhibit Plasmodium parasites. A strain of bacteria identified as Streptomyces hygroscopicus subsp. hygroscopicus strain i18 were isolated from pineapple fields in Lampung province, and was cultured and fermented on liquid synthetic Gause medium for 10 days. The supernatant was separated from the cells and extracted with ethyl acetate-methanol (1:1). Plasmodium falciparum 3D7 was used for antiplasmodial testing. Metabolites were tested qualitatively using a phytochemical approach. Saponins and triterpenoids were found to be present in the extract. Parasite inhibition as measured using probit analysis and yielded an IC50 value of 11.07 g.m/L. These findings suggest further examinations of this extract (e.g. assessment of off-target effects) are warranted. |
| first_indexed | 2024-12-17T19:52:52Z |
| format | Article |
| id | doaj.art-d6039589900d4e61b320eda83bb23aac |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-17T19:52:52Z |
| publishDate | 2021-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-d6039589900d4e61b320eda83bb23aac2022-12-21T21:34:41ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2021-06-0115289189610.22207/JPAM.15.2.45In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in LampungEndah SetyaningrumAchmad Arifiyantohttps://orcid.org/0000-0002-0224-6309Nismah NukmalTitik Nur Aenyhttps://orcid.org/0000-0002-2103-3560Meishy Handerlin PutriUlin Ni’mah SetiawatiIncreasing natural ingredient awareness and utilization has created an increased demand for sources of natural medicinal ingredients, including sources of compound used to treat malaria. Streptomyces is a genus of prokaryote well recognized for its production of antibiotics and other pharmaceutically useful compound. This study aimed to assess the ability of unpurified fermentation metabolites to inhibit Plasmodium parasites. A strain of bacteria identified as Streptomyces hygroscopicus subsp. hygroscopicus strain i18 were isolated from pineapple fields in Lampung province, and was cultured and fermented on liquid synthetic Gause medium for 10 days. The supernatant was separated from the cells and extracted with ethyl acetate-methanol (1:1). Plasmodium falciparum 3D7 was used for antiplasmodial testing. Metabolites were tested qualitatively using a phytochemical approach. Saponins and triterpenoids were found to be present in the extract. Parasite inhibition as measured using probit analysis and yielded an IC50 value of 11.07 g.m/L. These findings suggest further examinations of this extract (e.g. assessment of off-target effects) are warranted.https://microbiologyjournal.org/in-vitro-test-for-inhibition-of-plasmodium-falciparum-3d7-parasites-using-streptomyces-hygroscopicus-subsp-hygroscopicus-strain-i18-isolated-from-a-pineapple-farm-in-lampung/antiparasiticactinomycetesantimalarialparasitemicrobes |
| spellingShingle | Endah Setyaningrum Achmad Arifiyanto Nismah Nukmal Titik Nur Aeny Meishy Handerlin Putri Ulin Ni’mah Setiawati In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung Journal of Pure and Applied Microbiology antiparasitic actinomycetes antimalarial parasite microbes |
| title | In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung |
| title_full | In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung |
| title_fullStr | In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung |
| title_full_unstemmed | In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung |
| title_short | In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung |
| title_sort | in vitro test for inhibition of plasmodium falciparum 3d7 parasites using streptomyces hygroscopicus subsp hygroscopicus strain i18 isolated from a pineapple farm in lampung |
| topic | antiparasitic actinomycetes antimalarial parasite microbes |
| url | https://microbiologyjournal.org/in-vitro-test-for-inhibition-of-plasmodium-falciparum-3d7-parasites-using-streptomyces-hygroscopicus-subsp-hygroscopicus-strain-i18-isolated-from-a-pineapple-farm-in-lampung/ |
| work_keys_str_mv | AT endahsetyaningrum invitrotestforinhibitionofplasmodiumfalciparum3d7parasitesusingstreptomyceshygroscopicussubsphygroscopicusstraini18isolatedfromapineapplefarminlampung AT achmadarifiyanto invitrotestforinhibitionofplasmodiumfalciparum3d7parasitesusingstreptomyceshygroscopicussubsphygroscopicusstraini18isolatedfromapineapplefarminlampung AT nismahnukmal invitrotestforinhibitionofplasmodiumfalciparum3d7parasitesusingstreptomyceshygroscopicussubsphygroscopicusstraini18isolatedfromapineapplefarminlampung AT titiknuraeny invitrotestforinhibitionofplasmodiumfalciparum3d7parasitesusingstreptomyceshygroscopicussubsphygroscopicusstraini18isolatedfromapineapplefarminlampung AT meishyhanderlinputri invitrotestforinhibitionofplasmodiumfalciparum3d7parasitesusingstreptomyceshygroscopicussubsphygroscopicusstraini18isolatedfromapineapplefarminlampung AT ulinnimahsetiawati invitrotestforinhibitionofplasmodiumfalciparum3d7parasitesusingstreptomyceshygroscopicussubsphygroscopicusstraini18isolatedfromapineapplefarminlampung |